组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2008, Vol. 4 ›› Issue (6): 315-319.doi: 10.3969/j.issn.1673-0364.2008.06.005

• 论著 • 上一篇    下一篇

应用量子点标记小鼠胚胎干细胞的初步研究

皮庆猛，付炜，石伦刚，曹谊林，张文杰   

  1. 上海交通大学医学院附属第九人民医院整形外科组织工程国家工程中心;
  • 发布日期:2020-07-23

The Primary Study on Labeling of Mouse Embryonic Stem Cells with Quantum Dots

PI Qingmeng,FU Wei,SHI Lungang,CAO Yilin,ZHANG Wenjie   

  1. Department of Plastic and Reconstructive Surgery;Shanghai Ninth People s Hospital;Shanghai Jiaotong University School of Medicine;National Tissue Engineering Center;Shanghai 200011;China.;
  • Published:2020-07-23
  • Contact: 国家重点基础研究发展计划(2005CB522705);;国家自然科学基金(30571925,30671051)。

摘要： 目的探讨应用量子点(Quantum dots,Qdots)体外标记胚胎干细胞进行长期跟踪的可行性。方法以Qtracker 655标记小鼠未分化胚胎干(ES)细胞和小鼠胚胎成纤维(MEF)细胞,标记后倒置荧光显微镜和流式细胞仪动态观察细胞内Qdots的变化。丝裂霉素、秋水仙素或Verapamil处理标记Qdots的胚胎干细胞,观察细胞内Qdots含量与细胞增殖及多药耐药泵的关系。结果Qdots可以标记ES细胞,标记效率可达到90%以上。标记24h后细胞内Qdots含量较MEF细胞明显下降,至72h仅5%左右的ES细胞Qdots标记呈阳性。细胞增殖抑制实验显示,ES细胞内Qdots含量下降与细胞增殖关系不大。用Verapamil抑制多药耐药泵的功能,也不能阻止胞内Qdots含量的降低。结论Qdots不能长期标记未分化ES细胞,ES细胞内Qdots含量的迅速降低并非单由细胞增殖过快造成,而且与迅速地被排出细胞有关,但这一排出与多药耐药泵无关。

关键词: 细胞标记, 胚胎干细胞, 量子点

Abstract: Objective The aim of this study is to investigate the feasibility of labeling embryonic stem cells with quantum dots (Qdots) for long-term tracking in vitro. Methods Mouse embryonic stem cells (ES) and mouse embryonic fibroblasts (MEF) were labeled with Qtracker 655. After labeling, intra-cellular Qdots was detected by fluorescence microscope and flow cytometry. The inhibition of cell proliferation was carried out by mitomycin C or colchicines treatment after cell labeling, and the inhibiting of multi-drug ...

Key words: Cell labeling, Embryonic stem cells, Quantum dots

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