组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2014, Vol. 10 ›› Issue (6): 318-323.doi: 10.3969/j.issn.1673-0364.2014.06.005

• 论著 • 上一篇    下一篇

microRNA-21调控TGF-β通路促进增生性瘢痕形成的机制研究

张奇，王琛   

  1. 郸城县人民医院骨科；上海交通大学医学院附属第九人民医院整复外科
  • 发布日期:2020-07-23

Effect and M echanism of TGF-β Mediated Signal Pathway Modulated by microRNA-21 in Hypertrophic Scars

ZHANG Qi,WANG Chen   

  1. Department of Orthopaedics, People’s Hospital of Dancheng County; Department of Plastic and Reconstructive Surgery,Shanghai 9th People’s Hospital,Shanghai Jiaotong University School of Medicine;
  • Published:2020-07-23
  • Contact: 国家自然科学基金(81201204)；上海高校选拔培养优秀青年教师科研专项基金(ZZjdyx12100)

摘要： 目的探讨micro RNA-21在增生性瘢痕(Hypertrophic scars,HS)形成中的作用及机制,为生物学防治提供新靶点。方法收集临床患者正常皮肤及HS标本,组织学检测、组织胶原定量检测;免疫组化、Real-time PCR检测正常细胞外基质Col 1 A1、Col 3 A1、Fibronectin(FN)及α-SMA的表达;Real-time PCR检测micro RNA-21 m RNA表达水平;培养HS成纤维细胞,构建micro RNA-21反义表达载体,并加入TGF-β诱导,Real-time PCR检测Col 1 A1、Col 3A1及micro RNA-21的表达。结果 HS中胶原的含量及细胞外基质表达明显高于正常皮肤;HS组织中micro RNA-21表达高于正常皮肤组织;TGF-β可增强HS成纤维细胞中micro RNA-21的表达,而瘢痕成纤维细胞转染micro RNA-21反义表达载体后可明显降低瘢痕相关基因的表达。结论皮肤创伤愈合后局部micro RNA-21表达升高,促进了细胞外基质的合成,而TGF-β进一步加强了micro RNA-21对瘢痕形成的促进作用,可能是导致HS形成的重要原因。

关键词: 增生性瘢痕, 细胞外基质, 转化生长因子, 小分子核糖核酸

Abstract: Objective To evaluate the effect and mechanism of microRNA-21 in hypertrophic scars （HS） formation and to provide novel target in biological control of HS. Methods Normal skin tissue and HS tissue were harvested. Collagen quantitative detection and histological observation were carried out. The expression of collagen 1A1 （Col1A1）, collagen 3A1 （Col3A1）, Fibronectin （FN） and α-SMA were detected by quantitative real-time PCR. The expression of microRNA-21 mRNA in normal skin tissue and HS tissue were also detected. Cells isolated from normal skin tissues and HS tissues were cultured, the microRNA-21 antisense RNA interference vector were constructed, while TGF-β was added at the same time. Furthermore, the effects of inhibitor-microRNA-21 and TGF-β on the biological characteristics of fibroblasts were observed. Results The content of collagen in hypertrophic scar was higher than that in normal skin. The expression of extracellular matrix such as Col1A1, Col3A1, FN and α-SMA in scar tissue were significantly increased. The expression of microRNA-21 in HS tissue was higher than in normal tissue. TGF-βenhanced the expression of microRNA-21 in fibroblasts of HS tissues, while the inhibition of microRNA-21 decreased the expression of those scar-related genes. Conclusion The skin after wound healing could increase the expression of microRNA-21 which promotes the synthesis of extracellular matrix, and TGF-βmay further strengthen the role of microRNA-21 in the formation of HS.

Key words: Hypertrophic scar, Extracellular matrix, Transforming growth factor-β （TGF-β）, microRNA

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