组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2016, Vol. 12 ›› Issue (2): 94-97.doi: 10.3969/j.issn.1673-0364.2016.02.002

• 论著 •    下一篇

犬骨髓单个核细胞构建组织工程骨的体外实验研究

尹宏宇，丁榆德，刘广鹏，曹谊林   

  1. 中国医学科学院整形外科医院颌面整形外科中心；上海市第十人民医院整形外科；中国医学科学院整形外科医院
  • 发布日期:2020-07-23

Experimental Study of Constructing Tissue Engineered Bone with Canine Bone Marrow Mononuclear Cells in Vitro

YIN Hongyu,DING Yude,LIU Guangpeng,CAO Yilin   

  • Published:2020-07-23

摘要： 目的 探索组织工程骨(Tissue Engineered Bone,TEB)的即刻构建方案,以符合临床骨缺损即刻修复的要求。方法 分离获取Beagle犬骨髓单个核细胞(Canine bone marrow mononuclear cells,cBMNCs)。将密度为30×10~6 cells/mL的cBMNCs复合部分脱钙骨(Partially demineralized bone matrix,pDBM)即刻构建TEB。对即刻构建的TEB进行体外培养和成骨检测。结果 在成骨诱导的体外培养环境下,即刻构建的TEB具有一定的细胞活性和成骨能力。结论 即刻构建的TEB可直接回植,以满足体内即刻骨缺损修复的要求。

关键词: 组织工程骨, 骨髓单个核细胞, 骨髓基质干细胞

Abstract: Objective To explore a method for instantly constructed TEB to meet the instantly clinical requirements of repairing bone defects. Methods BMNCs of Beagle were separated by 1.063 g/mL Percoll gradient solution. The ideal seeding density of 30 ×106 cells/mL was obtained for canine bone marrow mononuclear cells (cBMNCs) on partially demineralized bone matrix (pDBM). The cell activity and osteogenic capacity of immediately constructed TEB composed of cBMNCs and pDBM were analyzed in vitro. Results Certain cell activity and osteogenic capacity were confirmed in immediately constructed TEB under the osteogenically inducted environment in vitro. Conclusion The directly replantation of instantly constructed TEB might satisfy the immediately clinical requirements of repairing bone defects.

Key words: Tissue engineered bone, Bone marrow mononuclear cells, Bone marrow mesenchymal stem cells

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