组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2010, Vol. 6 ›› Issue (6): 315-318.doi: 10.3969/j.issn.1673-0364.2010.06.004

• 论著 • 上一篇    下一篇

低氧状态下联合培养骨髓基质干细胞及其诱导来源的内皮细胞的实验研究

张超，曲伟栋，赵华强   

  1. 山东大学口腔医学院口腔颌面外科
  • 发布日期:2020-07-23

Experimental Study of Bone Marrow Stromal Cells and Induced Endothelial Cells in Co-Culture under Hypoxia

ZHANG Chao,QU Weidong,ZHAO Huaqiang   

  1. Department of Oral and Maxillofacial,Shandong University School of Stomatology,Jinan 250012,China
  • Published:2020-07-23

摘要： 目的在低氧条件下联合培养兔骨髓基质干细胞(Bone Marrow Stromal Cell,BMSC)及由其诱导而来的内皮细胞(Endothelial Cell,EC),观察并探讨低氧状态及EC对BMSC增殖的影响。方法从兔骨髓中分离获得BMSC,体外培养扩增后取部分向EC诱导。实验分4组:①BMSC常氧培养组;②BMSC低氧培养组;③BMSC+EC常氧联合培养组;④BMSC+EC低氧联合培养组。通过观察细胞的形态、增殖能力等了解低氧条件及EC对BMSC生长的影响。结果 BMSC可诱导为EC,EC与BMSC混合生长良好。MTT法检测显示,低氧培养组的细胞增殖能力明显高于常氧培养组(P<0.01),但相同条件下的BMSC单独培养组和BMSC+EC联合培养组间的细胞增殖能力没有显著性差异(P>0.05)。结论在一定时间内,低氧培养可以促进BMSC的增殖;BMSC来源的EC不能促进BMSC的增殖。

关键词: 骨髓基质干细胞, 内皮细胞, 低氧, 联合培养

Abstract: Objective To investigate the influence of hypoxia and co-culture of endothelial cells(EC) induced from BMSC and autologous bone marrow stromal cells(BMSC) on the proliferation of BMSC.Methods The rabbit BMSC were isolated and were induced into endothelial cells.These cells were divided into four groups.① BMSC group cultured under normoxia;② BMSC group cultured under hypoxia;③ BMSC+EC group co-cultured under normoxia;④ BMSC+EC group co-cultured under hypoxia.Inverted microscope and MTT method were applied to observe the effects of hypoxia and induced autologous EC on the cellular compatibility of BMSC.Results Endothelial cells could be induced from BMSC.Inverted microscope showed a cellular compatible growing status of BMSC and EC.The ability of proliferation of groups cultured under hypoxia were stronger than groups cultured under normoxia(P<0.01),but there was no significant difference between BMSC groups and BMSC+EC groups(P>0.05).Conclusion The hypoxia can increase the proliferation of BMSC.While the endothelial cells induced from BMSC can not.

Key words: Bone marrow stromal cell, Endothelial cell, Hypoxia, Co-culture

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