组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2009, Vol. 5 ›› Issue (1): 4-48.doi: 10.3969/j.issn.1673-0364.2009.01.002

• 论著 • 上一篇    下一篇

长期体外培养对人脐血间充质干细胞成骨分化潜能的影响

刘广鹏，张文杰，李宇琳，孙剑，周恒，崔磊   

  1. 上海组织工研究与开发中心;上海交通大学附属第九人民医院组织工程实验室
  • 发布日期:2020-07-23

The Effects of Long-term Culture In Vitro on the Osteogenic Differentiation Potential of Human Umbilical Cord Blood Derived Mesenchymal Stem Cells

LIU Guangpeng1,ZHANG Wenjie2,LI Yulin1,SUN Jian1,ZHOU Heng1,CUI Lei12   

  1. 1 Shanghai Tissue Engineering Research and Development Center;Shanghai 200235;China. 2 Tissue Engineering Key Laboratory;Shanghai Ninth People′s Hospital;Shanghai Jiaotong University School of Medicine;Shanghai 200011;China.;
  • Published:2020-07-23
  • Contact: 国家自然科学基金(30800232) 上海市科技启明星计划项目(07QA14053) 上海-英格兰东北地区科技合作计划(075407072)

摘要： 目的观察体外长期培养对人脐血间充质干细胞(Human umbilical cord blood derived mesenchymal stem cells,UCB-MSCs)成骨分化潜能的影响,探讨其作为组织工程骨种子细胞的可行性。方法流式细胞技术观察长期体外培养的UCB-MSCs细胞表面抗原表达的变化规律,并通过Alizarin Red染色及碱性磷酸酶活性、骨钙蛋白含量和钙离子含量的检测观察长期体外培养对UCB-MSCs成骨特性的影响。结果第10代之前的UCB-MSCs能够高表达间充质干细胞表面标志物,7代之前的细胞体外增殖能力不随传代次数而发生明显变化。Alizarin Red染色及碱性磷酸酶活性、骨钙蛋白含量和钙离子含量的检测显示8代之前的UCB-MSCs仍能保持较强的成骨分化能力。结论7代之前的UCB-MSCs能保持稳定的体外成骨分化特性,有望成为较理想的组织工程骨种子细胞。

关键词: 人脐血间充质干细胞, 组织工程, 成骨分化, 体外培养

Abstract: Objective To observe the effects of long-term culture in vitro on the osteogenic differentiation potential of human umbilical cord blood derived mesenchymal stem cells (UCB-MSCs). Methods The CD markers of UCB-MSCs were identified by flow cytometric analysis. Osteogenic differentiation of UCB -MSCs at different passages was evaluated by Alizarin Red staining, and measurement of alkaline phosphatase activity, osteocalcin content, and calcium content respectively. Results The CD marker expression of UCB-MSCs ...

Key words: Human umbilical cord blood derived mesenchymal stem cells, Tissue engineering, Osteogenic differentiation, Long-term culture in vitro

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