组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2009, Vol. 5 ›› Issue (4): 181-185.doi: 10.3969/j.issn.1673-0364.2009.08.001

• 论著 •    下一篇

不同培养基对Beagle犬骨髓基质细胞增殖与分化的影响

黄永玲，闫福华，钟泉，赵欣   

  1. 福建医科大学口腔医学院;福建医科大学口腔组织工程研究室;
  • 发布日期:2020-07-23

Effects of Media on the Proliferation and Differentiation of Bone Marrow Stromal Cells in Beagle Dogs

HUANG Yongling,YAN Fuhua,ZHONG Quan,ZHAO Xin   

  1. School of Stomatology;Tissue Engineering Research Center of Stomatology;Fujian Medical University;Fuzhou 350004;China.;
  • Published:2020-07-23
  • Contact: 国家自然科学基金(30471892) 福建省自然科学基金(2008J0085) 福建医科大学附属口腔医院重点学科建设学术发展基金[闽医大口腔(2008)39号]

摘要： 目的研究α-MEM、DMEM-LG、RPMI1640三种培养基对Beagle犬骨髓基质细胞(Bonemarrow stromalcells,BMSCs)体外培养生物学行为的影响,为BMSCs的培养寻找合适的培养基。方法取6只Beagle犬的BMSCs,分别用α-MEM、DMEM-LG、RPMI1640三种培养基进行培养,比较3组细胞的贴壁﹑增殖及矿化等方面的差异。结果α-MEM组24h内贴壁的细胞数目最多;消除贴壁差异后,α-MEM和DMEM-LG促增殖作用相近;α-MEM组碱性磷酸酶(Alkaline phosphatase,ALP)表达量最高,但矿化结节形成数目较少,DMEM-LG组矿化结节形成个数最多。结论DMEM-LG培养基较适合BMSCs的培养。

关键词: 骨髓基质细胞, 组织工程, 培养基, 增殖, 分化

Abstract: Objective To investigate the effects of different media: α-MEM, DMEM-LG and RPMI 1640 on the biological behavior of bone marrow stromal cells (BMSCs) in Beagle dogs, and provide the more ideal culture medium for BMSCs. Methods BMSCs derived from 6 Beagle dogs were harvested and grown in α-MEM, DMEM-LG and RPMI 1640 media respectively. The proliferation, attachment rate and mineralized nodules of the cells were observed and compared. Results Cells cultured by α-MEM showed the highest attachment rat. After el...

Key words: Bone marrow stromal cells, Tissue engineering, Media, Proliferation, Differentiation

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