组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2009, Vol. 5 ›› Issue (4): 199-207.doi: 10.3969/j.issn.1673-0364.2009.08.005

• 论著 • 上一篇    下一篇

SFRP2与Periostin的相互作用在瘢痕疙瘩形成机制中的研究

王珍祥，袁顺宗，陶熹，李世荣，吴军   

  1. 第三军医大学附属西南医院整形外科;
  • 发布日期:2020-07-23

Experimental Study of Interaction between SERP 2 and Periostin in the Formation of Keloid

WANG Zhenxiang,YUAN Shunzong,TAO Xi,LI Shirong,WU Jun   

  1. Department of Plastic Surgery and Institute of Burn;Southwest Hospital;Affiliated Third Military Medical University;Chongqing 400038;China.;
  • Published:2020-07-23
  • Contact: 国家重点基础研究发展规划项目(G1999054204)

摘要： 目的利用pull-down技术验证凋亡相关蛋白基因SFRP2(Secreted frizzled-related protein2)和骨母细胞特异性因子-2Periostin(Osteoblast-specific factor2)间的相互作用。方法构建能在哺乳动物细胞中表达带HA标签的Periostin融合蛋白(HA-Periostin)的重组载体pCMV-HA-Periostin,经酶切鉴定正确后,和表达带Myc标签的融合蛋白(Myc-SFRP2)的重组真核表达载体pCMV-HA-SFRP2,单独或共转染人293细胞,利用pull-down技术验证Periostin与SFRP2间的相互作用。结果成功构建重组载体pCMV-HA-Periostin,与抗Myc单克隆抗体沉淀Myc-SFRP2相互作用蛋白复合物后,可以检测到HA-Periostin的表达。通过体外蛋白质结合实验证实了Periostin与SFRP2间的相互作用。结论成功构建带HA标签的Periostin融合蛋白(HA-Periostin)的重组载体,利用pull-down技术证实了Periostin与SFRP2间的存在相互作用。

关键词: 凋亡相关蛋白基因, 骨母细胞特异性因子-2, 相互作用

Abstract: Objective To investigate the interaction between Periostin (osteoblast-specific factor 2) and SFRP 2 (Secreted frizzled-related protein 2) in the formation of keloid. Methods HA-tagged fusion protein (HA- Periostin) expression vector was constructed, identified and transfected into human embryo kidney 293 (HEK293) cells alone or with Myc-tagged fusion protein (Myc-SFRP 2) expression vector pMCV-Myc-SFRP 2. The interaction between SFRP 2 and Periostin was detected by pull-down technique. Results Double restr...

Key words: Secreted frizzled-related protein 2, Periostin, Interaction

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