组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2014, Vol. 10 ›› Issue (4): 207-210.doi: 10.3969/j.issn.1673-0364.2014.04.010

• 论著 • 上一篇    下一篇

小口径聚乳酸-己内酯/纤维蛋白原管形支架的构建及生物相容性评价

方征东，胡何节，董智慧，王晓天，孙小杰，葛新宝   

  1. 安徽医科大学附属省立医院血管外科；复旦大学附属中山医院血管外科
  • 发布日期:2020-07-23

Establish ment and Biocompatibility of a Small Diameter Tubular Scaffold of Electrospun Poly (L-lactide-co-ε-caprolactone)/fibrinogen Blended Fibers

FANG Zhengdong,HU Hejie,DONG Zhihui,WANG Xiaotian,SUN Xiaojie,GE Xinbao   

  1. Department of Vascular Surgery, Provincial Hospital Affiliated to Anhui Medical University; Department of Vascular Surgery, Zhongshan Hospital, Fudan University;
  • Published:2020-07-23
  • Contact: 安徽省自然科学基金（11040606m199，1208085mh151）

摘要： 目的探索静电纺丝技术制备小口径聚乳酸-己内酯[P(LLA-CL)]/纤维蛋白原管形支架的方法,评价支架的生物相容性,探讨其作为血管组织工程材料的可行性。方法以P(LLA-CL)、纤维蛋白原为原料,制备小口径复合管形支架,观察支架的大体形态,并用扫描电镜观察三维结构;利用溶血试验、细胞毒性试验、皮下植入试验,评价支架材料的生物相容性。结果管形支架表面呈网格状三维结构,并有大小不等、互相交通的孔隙,孔径平均直径为(4.56±1.23)μm,表面纤维平均直径(318±56)nm;P(LLA-CL)/纤维蛋白原浸提液溶血率为2.87%±0.49%;细胞毒性实验示P(LLA-CL)/纤维蛋白原浸提液较阴性对照组无明显差异(P>0.05);皮下植入试验显示P(LLA-CL)/纤维蛋白原支架炎症反应轻微,材料逐渐降解。结论通过静电纺丝技术可以构建小口径P(LLA-CL)/纤维蛋白原管形支架,并具有良好的生物相容性,可作为组织工程血管的支架材料。

关键词: 静电纺丝, 管形支架, 聚左旋乳酸-己内酯, 纤维蛋白原, 生物相容性

Abstract: Objective To fabricate a small diameter tubular scaffold of P (LLA-CL)/fibrinogen by electrospinning fiber technique, to evaluate the biocompatibility of the P (LLA-CL)/fibrinogen scaffold and its feasibility for vascular tissue engineering. Methods A small diameter tubular scaffold was fabricated by co-electrospinning blend of P (LLA-CL) and Fibrinogen. Gross morphology of the scaffold was observed and the 3-dimensional structure was observed by scanning electron microscope (SEM). Biocompatibilities of the tubular scaffold were evaluated in vivo and in vitro by acute hemolysis test, cytotoxicity test, and short-term test of subcutanous implantation. Results A randomly oriented nanofibrous structure with a well interconnected network of pores was observed. The diameter of the fiber at the outer surface was 318 ±56 nm and the average pore diameter was 4.56 ±1.23 μm; Hemolysis rate was 2.87%±0.49%; There was no significant difference of cytotoxicity between the P (LLA-CL)/fibrinogen tubular scaffold and negative control group (P>0.05). After the placement in rat subcutaneous pouches, the scaffolds were gradually biodegraded with little inflammatory reaction. Conclusion The P (LLA-CL)/fibrinogen tubular scaffold can be fabricated by electrospinning fiber technique. It has good biocompatibility and could be potentially used as vascular tissue engineering scaffold material.

Key words: Electrospinning, Tubular scaffold, Poly(L-lactide-co-ε-caprolactone), Fibrinogen, Biocompatibility

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