皮肤 , 　口腔黏膜 , 　单细胞测序 , 　成纤维细胞 , 　无瘢痕愈合

Objective　To explore the heterogeneity of human normal oral mucosa and skin fibroblasts and the mechanism of

oral mucosal fibroblasts in promoting scarless wound healing. Methods　Single-cell transcriptome sequencing data were obtained

from the GEO database for 13 cases of oral mucosa and 8 cases of skin. The 64,633 cells were subjected to quality control, dimen sionality, clustering and cell annotation. Fibroblasts were extracted and annotated for subsets definition, differential gene enrichment

and intercellular interactions analysis. Results　Fibroblasts can be divided into 5 subsets: inflammatory, adipose, mesenchymal,

contractile, and Wnt-inhibited. The proportion of inflammatory fibroblasts was significantly upregulated in the oral mucosa and

was associated with immune cell recruitment. CXCL13, EEF1A1 and B2M genes were highly expressed in oral mucosa fibro

blasts. Gene ontology (GO) functional analysis showed that scar healing-related pathways such as immune response and pro-in

flammatory pathways were activated in oral fibroblasts. Cellular interaction analysis revealed that the interaction between fibro

blast subsets was significantly changed, the information flow of oral mucosal COLLAGEN signaling pathway was upregulated, and

the score of collagen degradation genes including MMP1 and MMP10 were also increased (P<0.05). Conclusion　Fibroblasts

in normal skin and oral mucosa differ significantly in their subpopulation and function. The stronger inflammatory characteristics

and collagen metabolism of oral mucosal fibroblasts may drive the scarless wound healing.

Skin, 　Oral mucosa, 　Single cell sequencing, 　Fibroblast, 　Scarless healing