全层皮肤损伤的原位打印修复与毛囊重建

组织工程与重建外科杂志 ›› 2023, Vol. 19 ›› Issue (4): 336-.

全层皮肤损伤的原位打印修复与毛囊重建

出版日期:2023-08-01 发布日期:2023-09-05

In-situ bioprinting and hair follicle reconstruction of full-layer skin injury

Online:2023-08-01 Published:2023-09-05

摘要/Abstract

摘要：

　目的　利用原位打印的方式修复裸鼠背部皮肤缺损，实现损伤的原位修复和毛囊重建。方法　在裸鼠背部

构建直径 7 mm 的圆形全层皮肤缺损并覆盖海藻酸钙 / 氧化纳米细菌纤维素（CA/BC），将新生 C57 小鼠表皮 / 真皮

来源原代细胞作为种子细胞，每个创面植入 5×105 个细胞：实验组在 CA/BC 内原位打印 10 个高浓度细胞墨点；对

照组以 1 mL 注射器手工注射。术后 4 周观察创面恢复及毛发生长情况，通过 HE 染色检查新生毛囊结构。结果　皮

肤缺损实验组创面恢复良好，毛发生长旺盛、均匀，而对照组毛发稀疏、不均匀甚至没有毛发生长。组织学观察证实，

实验组新生皮肤内存在完整的毛囊结构，而对照组几乎没有毛囊形成。结论　初步验证了原位打印技术在皮肤缺损

原位修复和毛囊重建中的可行性。

关键词:

生物打印 , 　原位生物打印 , 　全层皮肤缺损 , 　毛囊重建

Abstract:

Objective　Using in-situ bioprinting to repair full-thickness skin wound on the back of nude mice to achieve

in-situ repairing and hair follicle reconstruction. Methods　Dorsal full-thickness skin defects (7 mm in diameter) was created

and covered with CA/BC in nude mice. About 5×105 primary cells from neonatal C57 mice skin were used as seed cells and

transplanted to the wound: 10 points of high-concentration cell plotting was printed in the CA/BC via in-situ bioprinting in ex

perimental group while 1 mL injector was used manually in control group. After 4 weeks, wound repairing and hair growth were

observed, and the structure of new hair follicles was examined by H&E staining. Results　The skin defect wound of the exper

imental group recovered well with vigorous and uniform hair growth, while the hair of the control group was sparse, uneven or

even no hair growth. HE staining showed that there was complete hair follicle structure in the new skin of the experimental group,

while there was almost no hair follicle formation in the control group. Conclusion　The feasibility of in-situ bioprinting tech

nology in skin defect repair and hair follicle reconstruction is preliminarily verified.

Key words:

　Bioprinting, 　In-situ bioprinting, 　Full-thickness skin defect, 　Hair follicle reconstitution

黎行宙, 陈骁俊, 孙梦哲, 等.

全层皮肤损伤的原位打印修复与毛囊重建

[J]. 组织工程与重建外科杂志, 2023, 19(4): 336-.

LI Xingzhou, CHEN Xiaojun, SUN Mengzhe, et al.

In-situ bioprinting and hair follicle reconstruction of full-layer skin injury [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2023, 19(4): 336-.