组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2023, Vol. 19 ›› Issue (4): 358-.

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阿克曼嗜黏液菌外膜蛋白 1100 促进糖尿病大鼠创面愈合

  

  • 出版日期:2023-08-01 发布日期:2023-09-05

Akkermansia muciniphila outer membrane protein 1100 promotes wound healing in diabetic rats

  • Online:2023-08-01 Published:2023-09-05

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摘要：

　目的　探究阿克曼嗜黏液菌外膜蛋白 1100（Amuc_1100）对糖尿病大鼠创面炎症及胶原纤维合成的影响。
方法　20 只雄性 SD 大鼠腹腔注射链脲佐菌素诱导为糖尿病大鼠，构建背部全层皮肤缺损创面模型并分为 2 组 ：对
照组及 Amuc_1100 组。Amuc_1100 组在创面局部多点注射 300 μL 浓度为 333 μg/mL 的 Amuc_1100 溶液，对照组注
射等量生理盐水。第 0、3、7、10、14 天创面拍照比较创面愈合率。第 7、14 天每组各处死 5 只大鼠并创面取材，
HE 染色评估创面炎症细胞浸润及组织学形态 ；Masson 染色评估创面胶原纤维沉积情况 ；RT-PCR 比较肿瘤坏死因
子 -α（TNF-α）、白细胞介素 -6（IL-6）、白细胞介素 -10（IL-10）、胶原纤维 1A1（Col1A1）、胶原纤维 3A1（Col3A1）
mRNA 相对表达量。结果　造模后第 3、7、10、14 天，Amuc_1100 组创面愈合率均高于对照组（P<0.05）；HE 染色
显示 Amuc_1100 组创面组织中炎症细胞聚集更少，胶原纤维沉积更多 ；Masson 染色显示 Amuc_1100 组创面胶原更
为粗大、深染，排列更为致密；RT-PCR 显示 Amuc_1100 组创面内 TNF-α、IL-6 mRNA 相对表达较对照组减少（P<0.05），
IL-10、Col1A1、Col3A1 mRNA 相对表达水平较对照组增多（P<0.05）。结论　Amuc_1100 能够提升糖尿病大鼠创面
愈合速率，其机制可能与降低局部炎症反应，增加胶原纤维合成有关。

关键词: 阿克曼嗜黏液菌外膜蛋白 1100 , 　糖尿病创面 , 　创面愈合 , 　炎症 , 　胶原纤维

Abstract:

Objective　To investigate the effects of Akkermansia muciniphila outer membrane protein 1100 (Amuc_1100)
on wound inflammation and collagen fiber synthesis in diabetic rats. Methods　Twenty male SD rats were induced into diabeticrats by intraperitoneal injection of streptozotocin. The defective wound model of full-thickness back skin was constructed and the
rats were divided into two groups, control group and Amuc_1100 group. The Amuc_1100 group was injected locally with 300 μL
Amuc_1100 solution with a concentration of 333 μg/mL, the control group was injected with the same amount of normal saline.
Wounds were photographed and the wound healing rate were compared on day 0, 3, 7, 10 and 14, respectively. Five rats in each
group were sacrificed and harvested on day 7 and 14. Hematoxylin-eosin (HE) staining was used to evaluate inflammatory cellinfiltration and histological morphology of the wound. Masson three-color staining was used to evaluate collagen fiber deposition.
The mRNA expression levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), collagen fiber
1A1 (Col1A1) and collagen fiber 3A1 (Col3A1) were compared by real-time quantitative polymerase chain reaction (RT-PCR).
Results　The wound healing rate in Amuc_1100 group was higher than that in control group on day 3, 7, 10 and 14 (P<0.05).
HE staining showed that the Amuc_1100 group had less inflammatory cells and more collagen fiber deposition in wound tissue.
Masson three-color staining showed that the collagen in the Amuc_1100 group was thicker, deeper dyed and more compact
than control group. RT-PCR showed that the relative mRNA expressions of TNF-α and IL-6 were significantly decreased in
Amuc_1100 group compared with the control group (P<0.05), and the relative mRNA expressions of IL-10, Col1A1 and Col3A1
were significantly increased compared with the control group (P<0.05). Conclusion　Amuc_1100 can improve the wound healing rate of diabetic rats, and the mechanism may be related to reducing local inflammatory response and increasing collagen fiber
synthesis.

Key words:

Collagen fiber