组织工程与重建外科杂志 ›› 2024, Vol. 20 ›› Issue (3): 285-.

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内源性骨膜引导骨再生过程中信号通路富集及FZD家族动态表达研究

  

  • 出版日期:2024-06-01 发布日期:2024-07-05

Enrichment of signaling pathways and dynamic expression of the FZD family during endogenous periosteum-guided bone regeneration

  • Online:2024-06-01 Published:2024-07-05

摘要:

目的 研究内源性骨膜引导骨再生过程中信号通路富集及卷曲蛋白(FZD)家族动态表达。方法建立猪闭合肋骨膜引导下的骨再生模型,选择7个时间点采集骨膜和再生骨组织进行基因测序,分析差异基因筛选以及相关信号通路富集的情况。选取与骨再生过程中密切相关的FZD分子家族,检测并分析FZD各成员的动态表达变化及其表达相关性。结果 实验组相较于对照组,共有7个基因在建模后1个月内的不同时间点持续存在差异表达。差异基因富集显示,早期主要存在免疫反应,后实现组织发展和重塑。FZD家族各成员在不同时间点的表达存在波动,大多数成员的表达高峰在术后 3 d,而实验组的倍数改变高峰在术后 1个月最为明显,FZD1和 FZD2最高,且二者具有较高的相关性。结论 本研究筛选出的骨膜引导性骨再生过程中的差异基因以及相关信号通路,为进一步的分子机制研究提供了理论依据,而FZD分子家族动态表达变化在该过程中发挥着重要作用,其具体参与的生物机制尚需进一步研究验证。

关键词:

Abstract:

Objective To investigate the signaling pathway enrichment and dynamic expression of FZD family during
endogenous periosteum-guided bone regeneration. Methods A porcine closed rib periosteum-guided bone regeneration
model was established, and periosteum and regenerated bone tissues were collected at seven time points for gene sequencing,
differential gene screening and enrichment analysis of their related signaling pathways. The FZD molecular family, which is
closely related to the process of bone regeneration, was selected for focused analysis, and the dynamic expression changes of
each member of FZD and the expression correlation between them were detected and analyzed. Results A total of seven
genes were persistently differentially expressed at different time points within 1 month after surgery in the experimental group
compared with the control group. Differential gene enrichment showed that there was mainly an immune response in the early
stage, and tissue development and remodeling were achieved later. The expression of each member of the FZD family
fluctuated at different time points, and the expression peak of most members was 3 days after surgery, while the fold change
of the experimental group was more obvious than that of the control group at 1 month after surgery, and FZD1 and FZD2 were
the highest, and there was a high correlation between them. Conclusion The differential genes in the process of periosteum
guided bone regeneration and the related signaling pathways screened in this study provide a theoretical basis for further
molecular mechanism studies, while the dynamic expression changes of the FZD molecular family play an important role in
the process, and the specific biological mechanisms involved in the process need to be verified by further studies.

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