组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2024, Vol. 20 ›› Issue (4): 411-.

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自体表皮细胞悬液与网状自体刃厚皮移植联合治疗深Ⅱ度烧伤创面的临床研究

  

  • 出版日期:2024-08-01 发布日期:2024-09-11

Clinical study on the combined application of autologous epidermal cell suspension and meshed autologous split thickness skin graft in the treatment of deep partial thickness burns

  • Online:2024-08-01 Published:2024-09-11

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摘要：

目的　探索自体表皮细胞悬液联合网状皮移植用于深Ⅱ度烧伤创面的治疗效果。方法　将深Ⅱ度烧伤患者 随机分为试验组和对照组，对照组接受刃厚网状皮移植（n=8），试验组以自体表皮细胞悬液联合刃厚网状皮移植进行治 疗（n=8）。表皮细胞悬液取自患者自体健康皮肤组织，通过细胞分离器制备获得。统计两组创面愈合时间、治疗后1周 的创面愈合率，以及术后8周的瘢痕评分（温哥华量表），并检测制备的细胞悬液中的表皮细胞浓度、活率。结果　对照 组愈合时间（12.1±0.6） d，试验组愈合时间（10.2±0.3） d；治疗后 1 周，对照组愈合率为 76.97%±10.68%，试验组为94.85%±10.01%；术后 8 周，瘢痕温哥华评分对照组 6.13±3.44，试验组 2.92±1.07。各项指标试验组均明显优于对照组 （P＜0.05）。制备细胞悬液的细胞数量为（1.77±0.11）×106 个细胞/cm2 皮肤标本，细胞活率为 87.85%±4%。结论　本研 究初步证实了自体表皮细胞悬液联合网状皮移植用于深Ⅱ度烧伤创面治疗，可促进植皮愈合时间，减少供区面积，并可 改善术后的瘢痕增生。

关键词: 烧伤, 　植皮, 　自体皮肤细胞悬液

Abstract: Objective　To evaluate the effect of autologous epidermal cell suspension combined with mesh skin graft in deep II-degree burn wounds. Methods　Patients with deep partial thickness burns were selected as the research subjects and randomly divided into two groups， control group and experimental group， according to the random table method. Control group received split-thickness mesh skin graft（ n=8）， and experimental group received autologous epidermal cell suspension combined with split-thickness mesh skin graft（ n=8）. The epidermal cell suspension was prepared from autologous donor skin by cell separator. The wound healing time， wound healing rate on first week after treatment， and scar score （Vancouver scale） on 8th week after surgery were statistically analyzed in the two groups. At the same time， the concentration and viability of epidermal cells in the cell suspension were detected. Results　The healing time of experimental group was （10.2±0.3） days， compared with（ 12.1±0.6） days in control group（ P<0.05）. The healing rates of control group and experimental group were 76.97%±10.68% vs. 94.85%±10.01% （P<0.05） one week after treatment， and the Vancouver scar scores of control group and experimental group were 6.13±3.44 vs. 2.92±1.07 （P<0.05） at 8 weeks after surgery. The concentration of cell suspension was（ 1.77±0.11）×106 cells per cm² skin sample， and the cell viability was 87.85%±4%. Conclusion　This study preliminarily confirmed that autologous epidermal cell suspension combined with mesh skin transplantation in the treatment of deep partial thickness burns can promote skin grafting healing time， reduce donor area， and improve postoperative scar hyperplasia.

Key words: Burns, 　Skin grafting, 　Autologous epidermal cell suspension