组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2009, Vol. 5 ›› Issue (4): 190-194.doi: 10.3969/j.issn.1673-0364.2009.08.003

• 论著 • 上一篇    下一篇

利用人源化小鼠研究组织工程材料的体内免疫原性

张秋玉，盛慧明，崔磊，王瑛，尹烁，林锦骠，吴娟娟，沈佰华，王利，李宁丽   

  1. 福建医科大学免疫系;上海交通大学医学院;上海市免疫学研究所;上海组织工程研究与开发中心;
  • 发布日期:2020-07-23

Experimental Study of Immunogenicity of Tissue Engineering Materials by Using Humanized NOD/SCID Mice

ZHANG Qiuyu1,2,SHENG Huiming1,CUI Lei3,WANG Ying1,YING Shuo3,LIN Jinbiao1,WU Juanjuan1,SHEN Baihua WANG Li1,LI Ningli1   

  1. 1 Shanghai Jiaotong University School of Medicine;Shanghai Institute of Immunology;Shangh 200025;China. 2 Department of Immunology;Fujian Medical University;Fuzhou 350004;China. 3 Shanghai Tiss Engineering Research and Development Center;Shanghai 200235;China.;
  • Published:2020-07-23

摘要： 目的建立并利用人源化小鼠模型,研究组织工程材料的体内免疫原性。方法利用NOD/SCID小鼠(非肥胖型糖尿病鼠与重症联合免疫缺陷鼠反交鼠模型),通过腹腔注射人外周血单个核细胞(PBMC)(100×106)建立人源化小鼠,在人源化小鼠皮下植入异体人皮肤(阳性对照)、人体脂肪干细胞与脱钙骨构建的组织工程骨(实验组)、单纯脱钙骨材料(阴性对照)。在植入后1周、2周、3周、4周,取小鼠尾静脉血,流式细胞仪检测人CD4+和CD8+淋巴细胞比例。4周后,取小鼠的移植局部皮肤进行HE染色分析,同时检测脾细胞人CD4+和CD8+淋巴细胞比例。结果NOD/SCID小鼠腹腔注射人PBMC4周内,在外周血和脾中均可测到人CD4+和CD8+淋巴细胞。病理分析结果显示:在人源化小鼠皮下植入的组织工程材料及成体干细胞构建的组织工程骨组未见炎性细胞浸润;而单独植入人皮肤组可见明显的炎性细胞浸润。结论人源化小鼠可作为组织工程材料体内免疫原性的研究的良好模型。

关键词: 非肥胖型糖尿病鼠与重症联合免疫缺陷鼠反交鼠模型小鼠, 免疫原性, 组织工程材料

Abstract: Objective To investigate the immunogenicity of new transplanted materials in vivo by using humanize NOD/SCID mice model. Methods humanized mice model was established by transferred 100×106 human peripheral blo mononuclear cells (PBMC) into NOD/SCID mice. Next day, the mice were received the allotransplantation of pieces of huma skin from plastic surgery patients as positive control group and received tissue engineering materials (silica ge accompanying with human adipose -derived stem cells (ADSCs) as exper...

Key words: NOD/SCID mice, Immunogenicity, Tissue engineering material

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