Journal of Tissue Engineering and Reconstructive Surgery ›› 2009, Vol. 5 ›› Issue (2): 70-74.doi: 10.3969/j.issn.1673-0364.2009.04.003

• Original article • Previous Articles     Next Articles

An Experimental Study of Bone Mesenchymal Stem Cells Seeds into Small Intestinal Submucosa Induced Toward Bone In Vitro

ZHANG Kaigang1,ZENG Bingfang2,ZHANG Changqing2   

  1. 1 Department of Orthopedic Surgery;Taian Center Hospital;Shandong;271000;China;2 Department of Orthopedic Surgery;Shanghai Sixth People s Hospital;Shanghai Jiaotong University School of Medicine;Shanghai 200233;
  • Published:2020-07-23

Abstract: Objective To investigate the feasibility of bone mesenchymal stem cells(BMSCs) seeded into small intestinal submucosa(SIS) to form the tissue engineered bone through inducing culture.Methods The BMSCs were isolated by percoll gradient centrifugation from rabbit s bone marrow.The BMSCs were induced to become osteogenic cells in the defined medium,and were seeded on SIS as the scaffold material for the formation of cell-SIS composite.The scaffold-cell constructs were cultured in vitro for 1 week and were impl...

Key words: Tissue engineering, Small intestinal submucosa, Bone, Scaffold, Bone mesenchymal stem cells

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