Journal of Tissue Engineering and Reconstructive Surgery ›› 2015, Vol. 11 ›› Issue (2): 95-109.doi: 10.3969/j.issn.1673-0364.2015.02.012

• Original article • Previous Articles     Next Articles

Effect of Necrotic Cells on Proliferation and Migration of Surrounding Normal Vascular Smooth Muscle Cells and the Underlying Mechanisms

MENG Liang,GAO Yuan,CHEN Bin,MA Jian,LIU Yong,WANG Wei,ZHAO Yalin   

  1. Fengtian hospital, Shenyang medical college;The First Hospital of China Medical University;Shenyang The Fourth Hospital of People;Shenyang medical college;
  • Published:2020-07-23
  • Contact: 辽宁省科学技术计划项目(2011225020)

Abstract: Objective To observe the effect of necrotic vascular smooth muscle cells (VSMCs) on proliferation and migration of surrounding normal cells and to explore the possible mechanism. Methods VSMCs was cultured in oxygen-and glucose deprived medium and the supernatant was collected to intervene normally growing cells. The control group, NCS group, NCS+IL-1 RA group, NCS+IL-6 RA group, IL-1αgroup and IL-6 group were set in this experiment. CCK-8 and Transwell assays were used to determine the proliferation and migration of VSMCs. The mRNA expression of IL-6 and CRP were detected by RT-PCR, and the protein expression of CRP, Akt and p-Akt were evaluated by Western blot. Results Compared with the control group, the proliferation and migration ability of NCS group, IL-1αgroup and IL-6 group were significantly higher than that in the control group (P<0.05). Meanwhile, the expression of IL-6 were obviously increased in NCS group and IL-1αgroup (P<0.05), and the expression of CRP and p-Akt were also elevated in the three groups (P<0.05). Otherwise, the proliferation and migration ability of NCS+IL-1 RA group and NCS+IL-6 RA group were decreased and the expression of CRP and p-Akt were also declined (P<0.05). Conclusion Necrotic vascular smooth muscle cells may promoting the proliferation and migration of surrounding normal cells by inducing the expression of IL-6 and upregulating the expression of CRP and p-Akt.

Key words: Vascular smooth muscle cells, Necrosis, Proliferation,Migration

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