Journal of Tissue Engineering and Reconstructive Surgery ›› 2010, Vol. 6 ›› Issue (5): 248-252.doi: 10.3969/j.issn.1673-0364.2010.05.003

• Original article • Previous Articles     Next Articles

The Effect of IFN-γ Increasing MHC-Ⅱ Expressing to the Immunogenicity of Keratinocytes

SONG Rengang1,SONG Renqiang2,LIU Dalie3   

  1. 1 Department of Plastic Surgery,Shenzhen People′s Hospital,Shenzhen 518020,China;2 Jiamusi Municipal Center for Disease Control and Prevention,Jiamusi 154002,China;3 Department of Plastic Surgery,Zhujiang Hospital,Southern Medical University,Guangzhou 510282,China.
  • Published:2020-07-23
  • Contact: 国家自然科学基金(30570517)

Abstract: Objective To observe changes of immunogenicity of keratinocytes（KC） under the influence of interferon-γ（IFN-γ）.Methods KC were cultured and passaged in vitro.Different dose of IFN-γ stimulated KC to express MHC-Ⅱ molecules.KC with highty expressed MHC-Ⅱ molecules were mixed with allogenic lymphocytes to observe their proliferation.Simultaneously,MHC-Ⅱ+ KC were implanted subcutaneously into allogenic mice to observe the sign of immunological rejection.Results Under the influence of 6 000 U/mL IFN-γ,the percentage of MHC-Ⅱ+ KC exceeded 90%.MHC-Ⅱ molecules highly expressed KC had no effects to stimulate proliferation of allogenic lymphocytes significantly.There were no obvious lymphocytes infiltration when MHC-Ⅱ molecules highly expressed KC were implanted subcutaneously into allogenic mice.Conclusion Passaged KC could express MHC-Ⅱ molecules highty in the influence of IFN-γ,KC expressed MHC-Ⅱ molecules has no obvious immunogenicity.

Key words: IFN-γ, MHC-Ⅱ, Keratinocyte, Immunogenicity

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