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23 July 2020, Volume 6 Issue 5 Previous Issue    Next Issue

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Primary Study of the Expression of Embryonic Stem Cell Markers CD31 and SSEA-1 in Developing Embryos and Adult Tissues WANG Shujun,FU Wei,ZHANG Wenjie 2010, 6 (5):  241-243.  DOI: 10.3969/j.issn.1673-0364.2010.05.001 Abstract ( 460 )   Objective To detect the expression of CD31 and SSEA-1 at different stages of developing embryos,as well as their expressions in neonates,adult bone marrow and testis.Methods 129/SV mice were mated.Embryos at 10.5 postcoitus（E10.5）,14.5 and 18.5 days were collected.In addition,neonatal mice,bone marrow and testis from 6-week-old mice were also collected.Single cell suspension was achieved by digestion with collagenase.After anti-CD31 and anti-SSEA-1 antibody labeling,cells were analyzed by flow cytometry.Results CD31 and SSEA-1 were expressed in E10.5,E14.5 and E18.5.The expression levels were decreased with the development of embryos.Meanwhile,both markers were observed in neonates,adult bone marrow and testis,but the level was lower than in embryos.Conclusion A certain amount of CD31 and SSEA-1 expressing cells are existing in developing embryos as well as in adult tissues. Related Articles | Metrics

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Culture and Identification of Rat Bone Marrow Derived-Endothelial Progenitor Cells in Vitro ZHENG Shengwu,ZAN Tao,DING Li,ZHENG Qingjian,LIU Jianing,YANG Yu,LIN Genhui 2010, 6 (5):  244-247.  DOI: 10.3969/j.issn.1673-0364.2010.05.002 Abstract ( 344 )   Objective To investigate methods of culturing,differentiation and identification of rat bone marrow derived-endothelial progenitor cells（EPCs）.Methods The marrow mononuclear cells were gained from Wistar rat bone marrow by density gradient centrifugation,and cultured in microvascular endothelial growth medium-2.Cells were isolated with adherence screening method and differentiated to EPCs after induction.The procedure of EPCs growing and differentiating was observed.EPCs were identified by cellular morphology and cellular phaenotype and cellular function.Results After 3 days culture,cells nearly completed adhering to culture plates.After renovating culture fluid on the 4th day,cells started to form colonies.While cultured for 7 days to 8 days,cells were differentiated into endothelial-like cells and formed typical cobblestone-like structure.On the 7th day and 10th day,adherent cells were identified positive for CD34,Flk-1 and CDl33 by immunofluorescence staining.Observation using fluorescence microscopy,the double-positive staining with DIL-ac-LDL and FITC-UEA-1 cell population was above 75 percent among adherent cells.Conclusion The bone marrow mononuclear cells are gained by density gradient centrifugation,then via adherent culture in microvascular endothelial growth medium-2,EPCs are obtained and amplified in vitro. Related Articles | Metrics

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The Effect of IFN-γ Increasing MHC-Ⅱ Expressing to the Immunogenicity of Keratinocytes SONG Rengang,SONG Renqiang,LIU Dalie 2010, 6 (5):  248-252.  DOI: 10.3969/j.issn.1673-0364.2010.05.003 Abstract ( 415 )   Objective To observe changes of immunogenicity of keratinocytes（KC） under the influence of interferon-γ（IFN-γ）.Methods KC were cultured and passaged in vitro.Different dose of IFN-γ stimulated KC to express MHC-Ⅱ molecules.KC with highty expressed MHC-Ⅱ molecules were mixed with allogenic lymphocytes to observe their proliferation.Simultaneously,MHC-Ⅱ+ KC were implanted subcutaneously into allogenic mice to observe the sign of immunological rejection.Results Under the influence of 6 000 U/mL IFN-γ,the percentage of MHC-Ⅱ+ KC exceeded 90%.MHC-Ⅱ molecules highly expressed KC had no effects to stimulate proliferation of allogenic lymphocytes significantly.There were no obvious lymphocytes infiltration when MHC-Ⅱ molecules highly expressed KC were implanted subcutaneously into allogenic mice.Conclusion Passaged KC could express MHC-Ⅱ molecules highty in the influence of IFN-γ,KC expressed MHC-Ⅱ molecules has no obvious immunogenicity. Related Articles | Metrics

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Haploidentical Hematopoietic Stem Cell Transplantation and Mesenchymal Stem Cell Infusion for the Treatment of Metachromatic Leukodystrophy XUE Mei,WANG Zhidong,LIU Jing,ZHU Ling,DING Li,HAN Dongmei,YAN Hongmin,GUO Zikuan,WANG Hengxiang 2010, 6 (5):  253-256.  DOI: 10.3969/j.issn.1673-0364.2010.05.004 Abstract ( 397 )   Objective To investigate the effect of haploidentical hematopoietic stem cell transplantation and mesenchymal stem cell infusion in the treatment of metachromatic leukodystrophy.Methods A 7-year-old girl with metachromatic leukodystrophy received hematopoietic stem cell transplantation and mesenchymal stem cells infusion.The pretreatment regimen consisted of fludarabine,cyclophosphamide,and busulfan.The prophylaxis of acute graft-versus-host disease was performed by the administration of anti-CD25 monoclonal antibody,cyclosporine A,methotrexate,mycophenolate mofetil,anti-thymocyte globulin and G-CSF-mobilized bone marrow.After hematopoietic reconstruction,mesenchymal stem cells derived from umbilical cord were intravenously infused once a week for 4 consecutive weeks.Results The patient achieved rapid hematopoietic engraftment of donor origin.Neutrophil（> 0.5 × 109 / L） and platelet（> 20 × 109 / L） recovery was achieved on +11 day.On day 14,arylsulfatase A level in peripheral blood leukocytes was significantly increased and it reached to the normal range on +28 day.No acute graft-versus-host disease was observed.One month after transplantation,finger-printing analysis indicated an achievement of complete donor-type hematopoiesis.Neurological symptoms gradually recovered.Conclusion Haploidentical hematopoietic stem cell transplantation and mesenchymal stem cell infusion for the treatment of metachromatic leukodystrophy were safe,effective and leukocyte arylsulfatase A level rises rapidly.The novel regimen described here might be one of the options patients with metachromatic leukodystrophy and lack of HLA-matched sources. Related Articles | Metrics

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Clinical Analysis on the Treatment of Spinocerebellar Ataxia and Multiple System Atrophy-Cerebellar Type with Umbilical Cord Mesenchymal Stem Cells LIU Jing, XUE Mei, ZHU Ling, DING Li, YAN Hongmin, WANG Zhidong, HAN Dongmei, GUO Zikuan, WANG Hengxiang 2010, 6 (5):  257-260.  DOI: 10.3969/j.issn.1673-0364.2010.05.005 Abstract ( 598 )   Objective To observe the clinical effect of umbilical cord mesenchymal stem cells（UC-MSC） in the treatment of spinocerebellar ataxia（SCA） and multiple system atrophy-cerebellar type（MSA-C） by intrathecal injection.Methods From Oct 2009 to Sep 2010,14 cases with SCA and 10 cases of MSA-C were given UC-MSC by weekly intrathecal injection at a dose of 1×106 cells/kg for four times as a course.All the patients received 1 course of treatment except 3 cases received 2 cycles.The movement ability and the quality of daily life were evaluated with the International Cooperative Ataxia Rating Scale（ICARS） and Activity of Daily Living Scale（ADL） and the scores were compared with those before the treatment.Results The results showed that the scores were significantly decreased 1 month after treatment（P<0.01）.The symptoms,including unstable walking and standing,slow movement,upper limb fine motor disorders,writing difficulties and dysarthria,were greatly improved except one patient who had no response.The observed side effects included dizziness（4 patients）,back pains（2 cases） and headache（1 case）,which disappeared within 1-3 days.Conclusion UC-MSC implantation is safe,and able to ameliorate clinical symptoms and improve life quality of SCA and MSA-C patients to some extent as well. Related Articles | Metrics

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Effects on Maxillary Growth in Unilateral Cleft Alveolar of Animal Model CHEN Cheng, WU Lili, ZHAO Yu 2010, 6 (5):  261-264.  DOI: 10.3969/j.issn.1673-0364.2010.05.006 Abstract ( 310 )   Objective To establish an animal model of unilateral alveolar cleft similar with clinical patients and study the effects on the growth of maxilla during the period of rapid development.Methods Twenty dogs（7 weeks old） were divided into the control group（n=10） and the experimental group（n=10）.The dogs experimental group were operated to create a unilateral alveolar cleft.The data of maxillofacial morphology such as the length,width and height of the foreside,middle and back of maxilla via three-dimensional reconstruction CT to evaluate the effects on the growth of maxilla 12 weeks were measured after operation.Results The foreside and middle length,the foreside width and the foreside height of the maxillary were decreased in experimental group（P <0.05）.Conclusion The unilateral cleft alveolar without early intervention plays an important role in maxillary aberration. Related Articles | Metrics

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Surgical Treatment of Neurofibromatosis Type 1 by Multidisciplinary Schedule SHEN Yi, SUN Jian, LI Jun, ZHANG Chenping, ZHU Hanguang, TANG Yousheng, SHEN Guofang 2010, 6 (5):  265-268.  DOI: 10.3969/j.issn.1673-0364.2010.05.007 Abstract ( 344 )   Objective To explore the principles of surgical treatment in neurofibromatosis type 1（NF1）.Methods From May 1998 to August 2006,15 patients with NF1 were divided into small type and massive type.All patients were treated by multidisciplinary schedule.Results Each patient underwent operation for 1 to 5 times,and the average operation time was 2.6.Among 15 patients,4 patients underwent near-total resection,5 patients underwent subtotal resection,and 6 patients underwent partial resection.Except temporal facial nerve paralysis was found in 2 patients,salivary fistula and decreased vision was respectively found in 1 patient,other patients healed without obvious complications.During the post-operative follow-up periods of 1.5 to 6.5 years,no recurrences were developed in 4 patients underwent near-total resection.Recurrences in different degrees were found in other patients,but new cosmetic and functional disturbances have not been developed at present.Conclusion NF1 is a neurogenic disease.Complete resection for NF1 usually is impossible,but recurrence after subtotal or partial resection is common.We suggest NF1 should be treated by multidisciplinary schedule.Small type should be performed near-total resection.Massive type should be tried to improve functional and cosmetic disturbances when near-total resection could not be performed.Long-term follow-up and postoperative regular imaging are necessary to monitor the residual lesion. Related Articles | Metrics

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The Staged Reconstruction in Treatment of Adult Complete Treacher Collins Syndrome YIN Lin, TANG Xiaojun, YU Bing, NIU Feng, GUI Lai, ZHANG Zhiyong 2010, 6 (5):  269-297.  DOI: 10.3969/j.issn.1673-0364.2010.05.008 Abstract ( 262 )   Objective To explore the surgical management of Treacher Collins syndrome（TCS） which is a congenital craniofacial malformations affecting the structures derived from the first and second brachial arches.Methods The surgical approach is staged reconstruction,including upper facial reconstruction with specially designed outer calvarial table,mandibular lengthening by distraction osteogenesis technique,orthognathic surgery to correct the birdlike facial appearance and anterior open bite,as well as lipo-filling for the correction of residual depressive deformities.Results Two adult patients have undergone this protocol and the results were satisfactory.Conclusion The staged reconstruction is a good way to correct the deformity of the adult patient with complete Treacher Collins syndrome. Related Articles | Metrics

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Self-Comparative Study of Pulse Dye Laser for the Venular Malformation WANG Wei, LIN Xiaoxi, MA Gang, LI Wei,HU Xiaojie,JIN Yunbo,CHEN Hui,YANG Chuan,WANG Wei 2010, 6 (5):  272-275.  DOI: 10.3969/j.issn.1673-0364.2010.05.009 Abstract ( 369 )   Objective To investigate the therapeutic results of first and second generation PDL for port wine stain（PWS） and compare its difference.Methods Fifty patients（range 17-59 years） were treated with first and second generation of PDL.First generation of PDL was featured wit wave length 585 nm,5 mm spot size,pulse width 0.45 ms at frequency 5-6.5 J/cm2.Second generation of PDL was featured with wave length 595 nm,5 mm spot size,pulse width 1.5-3 ms at fluences 11-12.5 J/cm2,DCD was used dynamic cooling duration 20 ms,delay 20 ms.Results Based on chi-squared analysis,there was no statistically significant differences between first and second generation of PDL.Conclusion There was no significant difference of clinical outcome between the first and second generation PDL for the treatment of PWS.Cryogen spray cool equipment of second generation of PDL can improve the safety of PDL effectually. Related Articles | Metrics

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"Two-stage" Ear Reconstruction for Microtia WEI Jianjun, PAN Bo, YU Xiaobo, LIU Lei, ZHAO Yanyong, LIN Lin, ZHUANG Hongxing, JIANG Haiyue 2010, 6 (5):  276-278.  DOI: 10.3969/j.issn.1673-0364.2010.05.010 Abstract ( 540 )   Objective To explore the technique of Two-stage ear reconstruction for microtia.Methods Ear reconstruction was done using the "two-flap" technique and autogenous rib cartilage framework in the first stage.Almost all fine structures of ear,such as the crus,the tragus and the conchal cavity were reconstructed in the second stage.Results Seventy one patients of microtia underwent auricular reconstruction mainly by Two-stage ear reconstruction.All patients were followed up for 6 months-3 years after surgery.The colour of the reconstructed ears was in harmony with the facial skin;cartilage framework had no absorption and deformation.The reconstructed ears had excellent and three-dimensional contour and the cranio-auricular angle of the reconstructed ears bear strong resemblances to the opposite ears.Conclusion Two-stage ear reconstruction was a simple method with few complications and could be easily mastered. Related Articles | Metrics

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Analysis of Modified Keller Operation in Treating Hallux Valgus in 36 Cases YE Feilun, GAO Fulei 2010, 6 (5):  279-281.  DOI: 10.3969/j.issn.1673-0364.2010.05.011 Abstract ( 315 )   Objective To investigate clinical effect of modified Keller operation on hallux valgus.Methods According to preoperative evaluation of hallux valgus deformity,traditional Keller operation was modified in some aspects such as incision design,osteotomy of proximal phalanx,the mechanical balance on both sides of metatarsophalangeal joint and rehabilitation after surgery.From 2005 to 2009,62 feet of 36 patients were treated with modified Keller operation.All patients were followed up and the degree of deformity correction,the improvement of pain and the recovery of walking function were fully evaluated.Results All cases were followed up for 1 year to 3 years and nearly 92% of the patients had good results.Conclusion Modified Keller operation is an effective method for the patients with hallux valgus especially with severe deformity and can markedly improve the symptom and function of foot,but less complications. Related Articles | Metrics

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Application of Superficial Iliac Circumflex Artery Island Flaps in Repairing Defects of Perineal Region LIU Antang,JIANG Hua,ZHANG Jianlin 2010, 6 (5):  282-284.  DOI: 10.3969/j.issn.1673-0364.2010.05.012 Abstract ( 368 )   Objective To evaluate the clinical effects of superficial iliac circumflex artery（SICA） island flaps in repairing soft tissue defects of perineal region.Methods SICA island flaps were elevated and transposed to repair tissue defects of perineal region in 8 cases.Further local flaps transposition or splint thickness skin grafting were applied to recover the donor sites.Results Venous congestion happened in one case,and skin flap delay was performed in another case.All the flaps survived eventually and achieved satisfactory results in eight months to five years follow-up.Conclusion The use of SICA island flaps in the closure of soft tissue defects in perineal region is easy and practical,with the advantages of stable blood vessels,high survival rate and few complications. Related Articles | Metrics

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Removing Apocrine Sweat Glands and Keeping Subdermal Vascular Plexus by Mini-Invasive Incisions in Treating Bromhidrosis YANG Tao, YUAN Jie, LI Jun, YANG Dingwen 2010, 6 (5):  288-289.  DOI: 10.3969/j.issn.1673-0364.2010.05.014 Abstract ( 354 )   Objective To explore the effects and complications of removing apocrine sweat glands and keeping subdermal vascular plexus through mini-invasive incisions in treating bromhidrosis.Methods A retrospective analysis was done on 84 bromhidrosis patients who underwent operation of removing apocrine sweat glands and keeping subdermal vascular plexus with mini-invasive incisions.Results The operation was easy to handle.The odors in all the patients were decreased and there were a few complications after 6-20 months'' follow-up.Conclusion The treatment with removing apocrine sweat glands and keeping subdermal vascular plexus by mini-invasive incisions has a satisfactory outcome. Related Articles | Metrics

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Detachable Sleeve Circumcision Combined with Z-Plasty in Treating Phimosis WU Chunxin, CHENG Kaixiang, LIU Yang, WANG Shanliang 2010, 6 (5):  290-291.  DOI: 10.3969/j.issn.1673-0364.2010.05.015 Abstract ( 605 )   Objective To introduce a new method of circumcision combined with Z-plasty and its effect in treating phimosis by keeping the Buck’s and Colle’s fascia from removal.Methods After 1% lidocaine was injected superficially to the tunica albuginea around the penile root,the excess skin at both the inner and external lamina of the foreskin was removed while dartos was kept intact,except in case of a circumferential contracture,some longitudinal incision was made within the dartos to release the contracture.A Z-plasty was also used to prevent the circumferential contraction when suturing.Results Twenty cases of phimosis were treated by this method with a satisfactory result including less hemorrhage during operation,less swelling postoperatively and less complications.Conclusion The detachable sleeve circumcision with Z-plasty has the following superiorities in treating phimosis: less operative injury,less swelling after operation,no adhesion of the foreskin to the tunica albuginea,neither the contracture of the incisional scar. Related Articles | Metrics

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Treatment of Axillary Osmidrosis with the Dynamo-electric Shaver Technique YANG Yayi,JIANG Zhaohua,LI Shengli,CAO Weigang,SUN Baoshan 2010, 6 (5):  300-300.  DOI: 10.3969/j.issn.1673-0364.2010.05.013 Abstract ( 189 )   Related Articles | Metrics