Journal of Tissue Engineering and Reconstructive Surgery ›› 2009, Vol. 5 ›› Issue (1): 16-20.doi: 10.3969/j.issn.1673-0364.2009.01.005

• Original article • Previous Articles     Next Articles

Investigation on the Detection of Complement C3 and Its Neurobiological Character in Nerve Regeneration Conditioned Fluid

MENG Fanjun,LI Qingfeng,LI Yuping,XIE Feng,ZAN Tao,ZHENG Danning,WENG Rui,YANG Mei,LI Hua   

  1. Department of Plastic and Reconstructive Surgery;Shanghai Ninth People′s Hospital;Shanghai Jiaotong University School of Medicine;Shanghai 200011;China.;
  • Published:2020-07-23
  • Contact: 国家自然科学基金(30471795)

Abstract: Objective To explore the effects of promoting nerve regeneration of complement C3 in nerve regeneration conditioned fluid (NRCF). Methods The protein band of the relative molecular mass of (232-440)×103 in NRCF were analyzed by the Shotgun technique, liquid chromatography and mass spectrometry (LC-MS/MS). The primary cultured cortical neurons were exposed to different concentrations of exogenous complement C3 and nerve growth factor (NGF). Photograph of neurons in each group was taken every day, and the len...

Key words: Nerve regeneration conditioned fluid （NRCF）, Nerve regeneration, Complement C3, Neurite

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