Abstract:

Objective　To investigate the effects of strontium-β-tricalcium phosphate (SR-β-TCP) on the biological activ

ity and osteogenic differentiation of rabbit adipose derived stem cells (ADSCs). Methods　rADSCs were isolated and cultured

from subcutaneous fat of interscapular region of New Zealand white rabbits, and the third generation rADSCs were selected for

osteogenic induction. β-TCP materials and Sr-β-TCP materials were prepared, and the β-TCP materials and Sr-β-TCP materi

als were co-cultured with osteogenic induced rADSCs, respectively, and divided into 1% Sr-β-TCP group, 3% Sr-β-TCP group,

5% Sr-β-TCP group, β-TCP group and control group (osteogenic induction medium). The proliferation of rADSCs was detected

by MTT colorimetric assay, and the activity of Alkaline phosphatase (ALP) and calcium ion content were detected. The osteogen

ic related proteins of rADSCs were detected by Western blot (WB). Results　Compared with the control group and 3% Sr-β-

TCP group, the optical density (OD) value of β-TCP group, 1% and 5% Sr-β-TCP group decreased on the 1st, 3rd, 6th and 8th

day (P<0.05). The OD value of 5% Sr-β-TCP group was lower than that of β-TCP group and 1% Sr-β-TCP group on the 3rd,

6th and 8th day (P<0.05). OD values of all groups were increased with the extension of time (P<0.05). Compared with the control

group and β-TCP group, the calcium ion content, ALP activity, Notch3, pFAK, pERK1/2 and RhoA expression of 1% and 3% 

Sr-β- TCP groups were increased, while the calcium ion content, ALP activity, Notch3, pFAK, pERK1/2 and RhoA expression of 5%

Sr-β-TCP group were decreased (P<0.05). The calcium ion content, ALP activity, Notch3, pFAK, pERK1/2 and RhoA expression

of 3% Sr-β-TCP group were higher than those of 1% and 5% Sr-β-TCP group (P<0.05). Conclusion　3% Sr-β-TCP can improve

the biological activity and osteogenic differentiation of rADSCs, which may be related to Notch3/FAK/ERK1/2 signaling pathway.

Key words:

Strontium-β-tricalcium phosphate, 　Adipose derived stem cells, 　Osteogenic differentiation, 　Proliferation