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Table of Content

    01 June 2023, Volume 19 Issue 3 Previous Issue   
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    Transplantation of human induced pluripotent stem cell derived cardiac progenitor cells for acute myocardial infarction in rats

    LUO Runjiao, AI Xuefeng, LI Kaixiang, et al
    2023, 19 (3):  213. 
    Abstract ( 169 )   PDF (3951KB) ( 743 )  
    Objective To explore the effect of human induced pluripotent stem cell-derived cardiac progenitor cells trans
    plantation on acute myocardial infarction in rats. Methods Immunofluorescence staining was used to identify the pluripotency
    of human induced pluripotent stem cells, and GiWi method was used to differentiate them into cardiac progenitor cells in vitro,
    and then they were further differentiated into cardiomyocytes. Flow cytometry and immunofluorescence staining were used to
    evaluate the differentiation efficiency and morphology of the obtained cardiac progenitor cells and cardiomyocytes. The rat model
    of acute myocardial infarction was established by ligation of the left anterior descending coronary artery. TTC staining and echo
    cardiography were used to evaluate the model. Cardiac progenitor cells were transplanted into the marginal area of myocardial 
    infarction, and cardiac function was detected by echocardiography at 1 week and 4 weeks after operation. HE and Masson stain
    ing were used to evaluate the size of infarct area and the degree of fibrosis. Immunofluorescence staining was used to evaluate the
    survival and differentiation of cardiac progenitor cells in vivo. Results The stem markers OCT4, SOX2, Nanog and Tra-1-60
    were highly expressed in human induced pluripotent stem cells. The in vitro differentiation efficiency of cardiac progenitor cells
    was as high as 77.8%, expressing Isl1, a specific marker of cardiac progenitor cells. It can further differentiate into cardiomyo
    cytes with an efficiency of 83.3%, and high expression of cTnT, α-actinin and other myocardial specific markers. The rat model
    of acute myocardial infarction was successfully established. TTC staining showed that the infarct area was white and the left ven
    tricular ejection fraction was decreased. At 4 weeks after transplantation, compared with the model group, the cardiac function of
    the cell group was improved, ventricular dilatation was weakened, and fibrosis was alleviated. The transplanted cells survived in
    vivo and differentiated into cardiomyocytes. Conclusion Cardiac progenitor cells derived from human induced pluripotent stem
    cells have high differentiation efficiency in vitro, can survive and differentiate into cardiomyocytes after transplantation in vivo,
    inhibit ventricular remodeling and improve cardiac function.
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    Effect of Sr-β-TCP on biological activity and osteogenic differentiation of adipose derived stem cells
    WANG Ningning, ZHANG Shengmin, YAO Shubo, et al
    2023, 19 (3):  223. 
    Abstract ( 127 )   PDF (1455KB) ( 217 )  
    Objective To investigate the effects of strontium-β-tricalcium phosphate (SR-β-TCP) on the biological activity and osteogenic differentiation of rabbit adipose derived stem cells (ADSCs). Methods rADSCs were isolated and cultured
    from subcutaneous fat of interscapular region of New Zealand white rabbits, and the third generation rADSCs were selected for
    osteogenic induction. β-TCP materials and Sr-β-TCP materials were prepared, and the β-TCP materials and Sr-β-TCP materials were co-cultured with osteogenic induced rADSCs, respectively, and divided into 1% Sr-β-TCP group, 3% Sr-β-TCP group,
    5% Sr-β-TCP group, β-TCP group and control group (osteogenic induction medium). The proliferation of rADSCs was detected
    by MTT colorimetric assay, and the activity of Alkaline phosphatase (ALP) and calcium ion content were detected. The osteogenic related proteins of rADSCs were detected by Western blot (WB). Results Compared with the control group and 3% Sr-β-
    TCP group, the optical density (OD) value of β-TCP group, 1% and 5% Sr-β-TCP group decreased on the 1st, 3rd, 6th and 8th
    day (P<0.05). The OD value of 5% Sr-β-TCP group was lower than that of β-TCP group and 1% Sr-β-TCP group on the 3rd,
    6th and 8th day (P<0.05). OD values of all groups were increased with the extension of time (P<0.05). Compared with the control
    group and β-TCP group, the calcium ion content, ALP activity, Notch3, pFAK, pERK1/2 and RhoA expression of 1% and 3% 
    Sr-β- TCP groups were increased, while the calcium ion content, ALP activity, Notch3, pFAK, pERK1/2 and RhoA expression of 5%
    Sr-β-TCP group were decreased (P<0.05). The calcium ion content, ALP activity, Notch3, pFAK, pERK1/2 and RhoA expression
    of 3% Sr-β-TCP group were higher than those of 1% and 5% Sr-β-TCP group (P<0.05). Conclusion 3% Sr-β-TCP can improve
    the biological activity and osteogenic differentiation of rADSCs, which may be related to Notch3/FAK/ERK1/2 signaling pathway.
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     Effect of pressure therapy on hypertrophic scar based on body surface pressure sensor monitoring

    XU Jia, HAN Dong, SU Weijie, et al
    2023, 19 (3):  229. 
    Abstract ( 207 )   PDF (2025KB) ( 378 )  
    Objective To investigate the effects of strontium-β-tricalcium phosphate (SR-β-TCP) on the biological activ
    ity and osteogenic differentiation of rabbit adipose derived stem cells (ADSCs). Methods rADSCs were isolated and cultured
    from subcutaneous fat of interscapular region of New Zealand white rabbits, and the third generation rADSCs were selected for
    osteogenic induction. β-TCP materials and Sr-β-TCP materials were prepared, and the β-TCP materials and Sr-β-TCP materi
    als were co-cultured with osteogenic induced rADSCs, respectively, and divided into 1% Sr-β-TCP group, 3% Sr-β-TCP group,
    5% Sr-β-TCP group, β-TCP group and control group (osteogenic induction medium). The proliferation of rADSCs was detected
    by MTT colorimetric assay, and the activity of Alkaline phosphatase (ALP) and calcium ion content were detected. The osteogen
    ic related proteins of rADSCs were detected by Western blot (WB). Results Compared with the control group and 3% Sr-β-
    TCP group, the optical density (OD) value of β-TCP group, 1% and 5% Sr-β-TCP group decreased on the 1st, 3rd, 6th and 8th
    day (P<0.05). The OD value of 5% Sr-β-TCP group was lower than that of β-TCP group and 1% Sr-β-TCP group on the 3rd,
    6th and 8th day (P<0.05). OD values of all groups were increased with the extension of time (P<0.05). Compared with the control
    group and β-TCP group, the calcium ion content, ALP activity, Notch3, pFAK, pERK1/2 and RhoA expression of 1% and 3% 
    Sr-β- TCP groups were increased, while the calcium ion content, ALP activity, Notch3, pFAK, pERK1/2 and RhoA expression of 5%
    Sr-β-TCP group were decreased (P<0.05). The calcium ion content, ALP activity, Notch3, pFAK, pERK1/2 and RhoA expression
    of 3% Sr-β-TCP group were higher than those of 1% and 5% Sr-β-TCP group (P<0.05). Conclusion 3% Sr-β-TCP can improve
    the biological activity and osteogenic differentiation of rADSCs, which may be related to Notch3/FAK/ERK1/2 signaling pathway.
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     Clinical observation of subcutaneous buried transverse mattress suture on reducing high-tension incision scar

    MENG Fanjun, WANG Li, WANG Qiang, et al
    2023, 19 (3):  236. 
    Abstract ( 295 )   PDF (2328KB) ( 323 )  
    Objective To investigate the effect of subcutaneous buried transverse mattress suture on reducing high-tension
    incision scar. Methods Twelve patients with body surface pigmented nevus or local scar were selected, and the lesions were
    removed by outpatient operation. Then the superficial fascia layer was dissociated 1-1.5 cm to both sides of the incisal margin,
    and the incisions were sutured. The incisions were divided into modified suture group and traditional control group by self control
    method. In the modified suture group, subcutaneously buried transverse mattress suture was used to reduce tension and close
    the incision, while in the traditional suture group, subcutaneously buried vertical mattress suture was used to reduce tension and
    close the incision, and then interrupted suture was used to close the skin surface. Incision healing was observed during suture
    removal. Scar width was measured 6 months after operation, and the score of Vancouver scar scale was performed. Results
    Among the 12 patients, 10 patients completed the operation and incision healing observation. Scar measurement and evaluation
    were completed in 10 patients 6 months after the operation. Scar width in the modified suture group was (0.66±0.22) mm, smaller than the traditional control group [(0.98±0.24) mm], and the difference was significant (P<0.05). Vancouver scar scale score
    in the modified suture group was 1.70±0.55, also significantly lower than the traditional control group (3.07±0.97) with signif
    icant difference (P<0.05). Conclusion Subcutaneous buried transverse mattress suture can more fully relieve incision tension
    and reduce postoperative scarring than traditional tension-reducing suture method.
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    The incidence and risk factors of breast cancer-related lymphedema

    ZHANG Yingying, LI Hua, GUAN Jiaqin, et al
    2023, 19 (3):  242. 
    Abstract ( 466 )   PDF (1127KB) ( 1061 )  
    Objective To explore the incidence rate, severity and risk factors of breast cancer-related lymphedema (BCRL)
    among breast cancer survivors. Methods Data collected from 128 patients underwent breast cancer operation during September
    1 to December 31, 2019. All the patients were female, aging (49.0±6.8) years (29-81 years). The Norman questionnaire and arm
    circumference measurement were used to evaluate the BCRL status and treatment status of breast cancer patients from subjective
    and objective perspectives at 6, 12, 24 and 36 months after surgery separately. The influence of the correlation factors on BRCL
    occurrence was analyzed by Logistic analysis. Results BRCL incidences were 9.4%, 25%, 23.4% and 23.4% at 6, 12, 24, 36
    months according to arm circumference measurement, while 12.5%, 26.6%, 25.0% and 25.0% by Norman questionnaire. Among the 128 patients, 30 were diagnosed with BCRL after breast cancer surgery, among which the incidence of stage Ⅰ
    lymphedema, stage Ⅱ lymphedema and stage Ⅲ lymphedema were 15.6%, 6.3% and 1.6%, respectively. Age, body mass index (BMI)  radiotherapy, the number of positive lymph nodes and surgery method showed differences between the the BCRL group and control group. Both CDT or LVA showed improvement for the BCRL patients. Conclusion BCRL is a common complication for
    breast cancer patients after surgery. It can be fairly diagnosed in the first year after surgery without the increasing incidence of
    BCRL over time throughout 3 years. BMI, radiotherapy were found to be independent risk factors in the development of BCRL in
    this study.
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    Short-term effect of complex decongestion therapy on patients with lymphedema of both lower extremities after exploration of thoracic duct

    WEI Yapei, WANG Tingting, CHEN Yingying, et al
    2023, 19 (3):  249. 
    Abstract ( 200 )   PDF (918KB) ( 571 )  
    Objective To evaluate the effect of complex decongestion therapy on lymphedema of both lower extremities after
    exploration of thoracic duct. Methods The clinical data of 25 patients with lymphedema of both lower extremities from October
    2018 to September 2022 were reviewed. The patients received complex decongestion therapy after thoracic duct exploration were
    taken as the intervention group, and the patients did not receive complex decongestion therapy after thoracic duct exploration
    were taken as the control group.The peripheral diameter of affected limb and body composition analysis before and on the 7th day
    after treatment were evaluated. Results After 7 days of complex decongestion therapy for the affected limb, the circumference
    diameter of the 4 measuring points (the lower 1/3 of the lower leg, the upper 1/3 of the lower leg, the lower 1/3 of the thigh, and
    the upper 1/3 of the thigh) on the affected limb decreased significantly (P<0.05). The change rate of the lower 1/3 and upper
    1/3 of the lower leg circumference in the intervention group was significantly higher than that in the control group (P<0.05).
    Compared with before treatment, there was no significant difference in extracellular water content in the control group after
    treatment (P>0.05), while the extracellular water content in the intervention group was significantly decreased after treatment
    (P<0.05). Conclusion Complex decongestion therapy after exploration of thoracic duct has a good effect on patients with
    lymphedema of both lower extremities.
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    Axial pattern pedicled flaps in the repair of ankle soft tissue defects :

    case report and strategy analysis

    DING Hanwen, CHEN Yong, WANG Qian, et al
    2023, 19 (3):  253. 
    Abstract ( 191 )   PDF (1888KB) ( 414 )  
    Objective To investigate the clinical effect of different axial pattern pedicled flaps in the repair of ankle soft
    tissue defects. Methods From January 2017 to December 2020,four types of axial pattern pedicled flaps were used to repair
    ankle soft tissue defects in 22 patients. There were 13 medial plantar flaps, 2 sural neurofasciocutaneous flaps, 5 dorsal foot skin
    flap and 2 supra-ankle perforator flaps used in reconstruction. The flap size ranged from 3.0 cm×3.0 cm to 10.0 cm×5.0 cm.
    The donor sites were sutured directly or covered with skin graft. Results All flaps and donor flap grafts survived well. Patients
    were followed up for 6-24 months. Flaps had good appearance, soft texture and protective sensation. Conclusion According to
    the specific situation of the skin soft tissue defect around the ankle, combined with the characteristics of various pedicled axial
    flaps, the optimal selection can achieve expected effect.
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    Analysis of mutation type and clinical significance of congenital melanocytic nevi

    ZHAO Yifei, ZOU Yun, CHEN Hui, et al
    2023, 19 (3):  258. 
    Abstract ( 324 )   PDF (843KB) ( 1391 )  
    Objective To explore the molecular characteristics of giant congenital melanocytic nevi (GCMN) and their
    clinical significance. Methods The samples of patients diagnosed with GCMN were subjected whole-exome sequencing and
    bio-information analysis to clarify the pathogenic genes and distribution patterns. Results In 50 samples (25 pairs of tissues
    and blood) from 25 patients, 21 patients had somatic mutations, including NRAS gene (19 cases) and BRAF gene (1 case). New
    mutations were detected in 2 patients’
    lesions and blood, and the mutant genes were MET gene and IDH1 gene. In addition,
    no causative mutation was detected in 4 patients. There was no statistic difference between GCMN genotype and phenotype.
    Conclusion NRAS somatic mutation is the most common mutation in GCMN, and BRAF gene has also been detected. Potential
    new mutated IDH1 and MET genes were also found.
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    Study on the incidence of congenital genitourinary malformations in patients with congenital microtia patients

    GAO Dejin, WANG Bingqing, LIU Tun, et al
    2023, 19 (3):  265. 
    Abstract ( 117 )   PDF (1215KB) ( 310 )  
    Objective To study the incidence of congenital urogenital malformations in patients with congenital microtia,
    and to analyze the correlation between the two diseases. Methods A retrospective analysis was performed on 3 143 patients
    with microtia from January 2015 to December 2019, and the cases with genitourinary system malformations were counted
    and classified through renal ultrasound or abdominal CT examination. The incidence of congenital microtia combined
    with genitourinary system malformations and the side correlation between the affected ear and urogenital deformities were
    analyzed. Results Among 3 143 patients with congenital microtia, 163 cases were accompanied by congenital genitourinary
    system malformations, including unilateral kidney deficiency, renal hypoplasia, hydronephrosis, hypospadias, and testicular
    deformity, with a total incidence of 5.2%. Through data analysis, it can be seen that among the patients with congenital microtia
    accompanied by congenital genitourinary malformation, the proportion of second-degree microtia was the highest, and the
    proportion of hydronephrosis, renal cyst and testicular deformity was relatively high. What’s more, the side of congenital
    microtia was associated with the side of congenital genitourinary malformations. Conclusion In patients with congenital
    microtia, the incidence of genitourinary malformation is higher than in the general population, and they both are more likely to be
    ipsilateral. Such patients can be diagnosed through physical examination, renal ultrasound and abdominal CT, so as to focus on
    renal function in subsequent treatment to improve the prognosis of these patients.
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     Split retro-orbicularis oculi fat resection for correction of bulky upper eyelid in Asian blepharoplasty

    LI Hua
    2023, 19 (3):  270. 
    Abstract ( 162 )   PDF (1495KB) ( 506 )  
    Objective To explore the feasibility and effectiveness of the split retro-orbicularis oculi fat (ROOF) resection
    technique for the correction of bulky upper eyelid in double-eyelid surgery. Methods From 2018 to 2022, a total of 456 cases
    of double eyelid surgery with bulky upper eyelid were treated with the split ROOF resection technique. After fully dissection of
    ROOF pad, the fat was split from the anterior orbital septum, so as to completely preserve the anterior orbital septum while removing ROOF to the maximum extent. Meanwhile, appropriate amount of orbital septum fat and part of orbicularis oculi muscle
    were removed. The eyelid was sutured in layers so that the tension of the orbicularis oculi muscle does not directly act on the
    eyelid skin. There were 158 patients in 456 cases with different degrees of ptosis. They were corrected by levator palpebrae superioris shortening during the double eyelid blepharoplasty. Results The 456 patients were followed up for 3-48 months, with
    an average of 26.3 months. Among the 456 patients, 454 cases formed stable double eyelid fold, and two cases became shallow
    and disappeared 15 months and 18 months after surgery, respectively. The appearance of bulky eyelid was significantly improved
    in all patients, 20 patients had depigmentation at the surgical incision site, and no sunken scar, triple eyelid and other complications occurred. Conclusion Split ROOF removal combined with eyelid layering suture technique can effectively improve bulky
    upper eyelid, form a stable appearance of double eyelid, and prevent postoperative complications such as sunken scar and triple
    eyelid.
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    Application of stromal cell-derived factor-1 α combined with platelet-rich fibrin in bone regeneration of dental implants

    HUANG Liyun, LI Lin, XUE Rong, et al
    2023, 19 (3):  275. 
    Abstract ( 156 )   PDF (1192KB) ( 215 )  
    Objective To investigate the effect of stromal cell derived factor-1 α combined with platelet-rich fibrin on alve
    olar bone regeneration after dental implantation. Methods A total of 120 dental implant patients were selected from June 2019
    to December 2020. According to random number table method, patients were divided into experimental group (n=60) and con
    trol group (n=60). The control group was treated with platelet-rich fibrin + artificial bone meal, and the experimental group was
    treated with stromal cell-derived factor-1 α + platelet-rich fibrin + artificial bone meal. Bone mineral density, bone thickness,
    alveolar bone lip and tongue width, gingival health, implant success rate, complete healing rate, local infection rate, implant sta
    bility coefficient (ISQ), probing depth (PD) and gingival crevicular bleeding index (SBI) were compared between the two groups.
    Enzyme-linked immunosorbent assay and Western blot was used to detect the expression levels of platelet-derived growth factor
    (PDGF), transforming growth factor (TGF-β) and bone morphogenetic protein 2 (BMP-2) in gingival crevicular fluid and bone
    chips 7 days after operation. Results The expression levels of PDGF, TGF-β and BMP-2 protein in the experimental group
    were higher than those in the control group on the 7th day after surgery (P<0.05). The bone density, bone thickness, alveolar bone
    lip-lingual width and implant stability coefficient in the experimental group were higher than those in the control group at the 6th
    month after operation (P<0.05). The probing depth and gingival crevicular bleeding index were lower than those in the control 
    group (P<0.05). There was no significant difference in the implant success rate, complete healing rate and local infection rate be
    tween the two groups 6 months after surgery (P>0.05). Conclusion Stromal cell derived factor-1 α combined with platelet-rich
    fibrin can promote alveolar bone regeneration and repair in patients with dental implants, and the relevant mechanism may be
    related to the up-regulation of PDGF, TGF-β and BMP-2 expression.
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    A preliminary clinical study on the repair of acne vulgaris by traditional Chinese medicine external treatment

    WAN Yi, LI Yan
    2023, 19 (3):  281. 
    Abstract ( 191 )   PDF (1001KB) ( 445 )  
    Objective To observe the effect of traditional Chinese medicine external treatment on acne vulgaris. Methods
    Sixty patients with acne vulgaris were randomly divided into treatment group and control group. The treatment group was treated
    with a sharp needle for purulent drainage and bleeding, followed by a rolling needle for the introduction of traditional Chinese
    medicine solution. The control group was treated with oral tanshinone capsules combined with external application of clindamy
    cin solution. The acne grade, skin lesion hardness, efficacy, satisfaction, safety, and recurrence rate between the two groups were
    compared after treatment. Results After treatment, the treatment group showed better levels of acne, skin lesion hardness, skin
    repair efficacy, and satisfaction than the control group (P<0.05). There was no statistically significant difference in adverse reac
    tions between the two groups (P>0.05). The recurrence rate in the treatment group was lower than that in the control group (P<0.05).
    Conclusion Compared with the combination of oral administration of tanshinone and topical application of clindamycin solu
    tion, traditional Chinese medicine combined with external treatment has stronger repairing ability and better curative effect on
    acne vulgaris.
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     Application of digital information management on sterilized items in clinical operating scenarios: Practice and

    experience #br#

    JIANG Xue, HUANG Ying, YANG Xin, et al
    2023, 19 (3):  285. 
    Abstract ( 158 )   PDF (862KB) ( 561 )  
    Objective To explore the influence of digital information management mode on the time cost and reliability
    of sterilized items management in operating rooms. Methods The present study was performed basing on the sterilized items
    from the operating division, Department of Plastic and Reconstructive Surgery. A total of 1 790 sterilized instrument kits were
    collected from December 2021 to January 2022. According to the management, the kits were divided into two groups. The experimental group (n=910) adopted the digital information management mode, while the control group (n=880) adopted the conventional ledger management mode. The item-registering time, the validity-checking time, the storage cabinet settling time and
    the item-taking time were recorded in both groups. A group t test was applied to evaluate the difference between the two groups.
    Meanwhile, the reliability of the two groups, including sterilized item expiration, loss, and mistaking delivery, were also recorded
    and analyzed with Chi-square test. The satisfaction ratio of the medical staff was evaluated at the same time. Results Of the
    experimental group, the validity-checking time, the storage cabinet settling time and the item-taking time were significantly less
    than those of the control group (P<0.001). The item-registering time of the experimental group was significantly longer than that
    of the control group, but the total management time was still better than that of the control group (P<0.001). The item expiration
    rate, the item loss rate and the mistaking delivery rate of the experimental group were all lower than those of the control group 
    (P<0.01). Meanwhile, the satisfaction of medical staff in the experimental group was higher than that in the control group (P<0.05).
    Conclusion Compared with the traditional ledger management mode of sterilized items in the operating room, the digital information management mode could effectively reduce the time cost in the management process, obtain higher satisfaction of medical
    staff, and give a better clinical application.
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    Application analysis of digital surgical planning procedure in the reconstructive treatment of maxillofacial fracture

    GUAN Yaqin, WU Jinyang, YU Liya, et al
    2023, 19 (3):  289. 
    Abstract ( 123 )   PDF (1154KB) ( 367 )  
    Objective To explore the effect of digital surgical planning in the precise treatment of maxillofacial fractures
    and to summarize the digital workflow. Methods The clinical data of 40 patients with maxillofacial fractures who underwent
    preoperative digital surgical planning at the Department of Oral Craniomaxillofacial Surgery from January 2019 to December
    2022 were retrospectively analyzed to summarize the surgical planning procedure in the reconstructive treatment of maxillofa
    cial fractures. Results All 40 patients completed all preoperative examinations on the day of the scheduled surgical planning,
    and the average examination time consumed was less than 3 hours. All the operations were successfully completed within the
    scheduled time, the postoperative function and shape were well reconstructed, and patients were satisfied with the postoperative
    results. Conclusion A standardized digital surgical planning procedure can optimize the examination process for patients with
    maxillofacial fractures, improve the efficiency of doctors and nurses, and improve the surgical precision, which is an important
    part to ensure the expected results
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     Effect of standardized preoperative visit on repair of facial paralysis

    WANG Hui, GONG Liqing, GUO Hongfang, et al
    2023, 19 (3):  294. 
    Abstract ( 111 )   PDF (1000KB) ( 163 )  
    Objective To explore the effect of standardized preoperative visit in patients undergoing facial paralysis repair.
    Methods Eighty patients with facial paralysis were divided into control group and observation group according to the operation
    time. Forty patients admitted first were in the control group. The patients in control group was admitted to the hospital for surgery
    according to the traditional process. And 40 patients admitted later were in the observation group. The observation group imple
    mented the standardized preoperative visit method according to the newly improved process. The Self-rating Anxiety Scale (SAS)
    and Self-rating Depression Scale (SDS) were compared between the two groups. The average length of preoperative preparation
    time, the operation completion rate and the average length of stay were also observed. Results The SAS score of the observation
    group was 31.93±2.43 and SDS score was 31.525±2.82, which were significantly lower than the SAS score (46.03±2.31) and
    SDS score (44.02±2.06) of the control group (P<0.001). The preoperative preparation time of the observation group was (2±0)
    days, which was significantly less than that of the control group, which was (2.41±0.9) days (P<0.01). The operation completion
    rate of the observation group (97.5%) was higher than that of the control group (90%). The average hospital stay of the observation
    group was (6.45±1.31) days, which was lower than that of the control group, which was (8.08±2.23) days (P<0.05). Conclusion
     The application of standardized preoperative visit method can provide early, timely and effective assessment and nursing in
    tervention of systemic risks associated with repair of facial paralysis, alleviate patients’
    anxiety and depression, screen operation
    risks, shorten the preoperative preparation time after admission, improve the operation completion rate, and shorten the average
    hospital stay of patients. It is worthy of clinical promotion.
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    Research progress of three dimensional bio-printing technology for formation of vascular network in bone tissue

    ZHAI Chuanxing, WANG Zhimeng, WANG Guodong, et al
    2023, 19 (3):  298. 
    Abstract ( 177 )   PDF (913KB) ( 1035 )  
    Vascularization of bone grafts is one of the major challenges facing bone tissue engineering (BTE). The ultimate
    goal of the construction of vascularized BTE is to provide a bone environment rich in functional vascular networks to achieve
    efficient bone integration and accelerate the functional recovery after implantation. In this paper, the research progress of 3D
    bio-printing in the formation of vascular network in bone tissue was reviewed, the formation mechanism of vascular network and
    the characteristics of 3D bio-printing technology were elaborated, the current vascularization strategies in BTE were summarized,
    and its development prospects were prospected.
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    Research progress of 3D bioprinting technology in dermal regeneration

    XU Pengqin, HAN Chunmao
    2023, 19 (3):  302. 
    Abstract ( 305 )   PDF (973KB) ( 912 )  
     Soft tissue defects of skin are common in clinical practice. The restoration of dermal damage, to a large ex
    tent, contributes to the repairing effects and scar formation of skin soft tissue defects. Three-dimensional (3D) bioprinting is an
    emerging technology that can accurately distribute biomaterials and living cells. It creates intricate stereoscopic structures which
    facilitates regenerative repair of dermal defects. Due to limitations of printing method, bio-ink, and seed cells, 3D bioprinting
    technology for skin is still in the lab stage. With the development of 3D bioprinting, especially in novel bio-ink exploitation and
    technical optimization, high resolution 3D bioprinting will enable us to customize elaborate functionalized skin models and even
    cutaneous appendages, providing new strategies in dermal regenerative repairment.
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     Research progress of tissue engineering ligament and scaffold materials

    ZHONG Rui, GAO Jingyu, YAN Chengyuan, et al
    2023, 19 (3):  308. 
    Abstract ( 236 )   PDF (927KB) ( 1220 )  
     The reconstruction scheme of the existing artificial ligament products has been relatively mature in clinical ap
    plication. In order to prevent the occurrence of postoperative complications such as difficult tendon-bone healing, expansion of
    the bone tunnel, synovial inflammatory reaction, infection, and external fixation loosening after ligament reconstruction, tendon
    tissue construction using tissue engineering technology has become a possible development direction in the future. In this paper,
    the progress of the construction of tissue-engineered tendon and its scaffold materials was reviewed, the urgent problems to be
    solved and the future development direction were discussed.
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     Research progress of traumatic optic neuropathy

    LI Xinyu, GUO Zhilin
    2023, 19 (3):  315. 
    Abstract ( 248 )   PDF (791KB) ( 949 )  
     Traumatic optic neuropathy (TON) often occurs after cerebral injury and is mainly in young people. Optic nerve
    injury can lead to vision loss in mild cases and loss of vision in severe cases, seriously affecting the physical and mental health
    and working life of patients. Currently, there are different opinions on the treatment of TON, both surgical and non-surgical, and
    there is no method that is satisfactory to doctors and patients. In this paper, the recent advances in the study of optic nerve injury
    were reviewed in order to provide new ideas for clinical treatment.
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     Soft tissue anatomy and safe spaces for injectable filler placement in forehead region

    GU Tianyi, TENG Li
    2023, 19 (3):  319. 
    Abstract ( 122 )   PDF (1185KB) ( 694 )  
     Due to the advantages like less trauma, quicker recovery and relatively uncomplicated operation, more and more
    beauty seekers prefer injectable fillers to improve the appearance of the forehead. Completely understanding the complex anato
    my of the forehead region is helpful to avoid severe complications such as vascular occlusion, and to improve patient satisfaction.
    In this paper, the relevant literature was reviewed to summarize the systematic anatomy knowledge on forehead region and the
    safe spaces for forehead augmentation, to provide sufficient theoretical support for clinical injection and filling of forehead region.
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     Overview of sensitive skin and treatment progress of aesthetic medicine

    LU Nan, WU Min, SONG Jiao, et al
    2023, 19 (3):  323. 
    Abstract ( 303 )   PDF (708KB) ( 1544 )  
    敏感性皮肤 ; 机制 ; 诊断 ; 治疗 ; 光电治疗
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