Journal of Tissue Engineering and Reconstructive Surgery ›› 2023, Vol. 19 ›› Issue (4): 358-.

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Akkermansia muciniphila outer membrane protein 1100 promotes wound healing in diabetic rats

  

  • Online:2023-08-01 Published:2023-09-05

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Abstract:

Objective　To investigate the effects of Akkermansia muciniphila outer membrane protein 1100 (Amuc_1100)
on wound inflammation and collagen fiber synthesis in diabetic rats. Methods　Twenty male SD rats were induced into diabeticrats by intraperitoneal injection of streptozotocin. The defective wound model of full-thickness back skin was constructed and the
rats were divided into two groups, control group and Amuc_1100 group. The Amuc_1100 group was injected locally with 300 μL
Amuc_1100 solution with a concentration of 333 μg/mL, the control group was injected with the same amount of normal saline.
Wounds were photographed and the wound healing rate were compared on day 0, 3, 7, 10 and 14, respectively. Five rats in each
group were sacrificed and harvested on day 7 and 14. Hematoxylin-eosin (HE) staining was used to evaluate inflammatory cellinfiltration and histological morphology of the wound. Masson three-color staining was used to evaluate collagen fiber deposition.
The mRNA expression levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), collagen fiber
1A1 (Col1A1) and collagen fiber 3A1 (Col3A1) were compared by real-time quantitative polymerase chain reaction (RT-PCR).
Results　The wound healing rate in Amuc_1100 group was higher than that in control group on day 3, 7, 10 and 14 (P<0.05).
HE staining showed that the Amuc_1100 group had less inflammatory cells and more collagen fiber deposition in wound tissue.
Masson three-color staining showed that the collagen in the Amuc_1100 group was thicker, deeper dyed and more compact
than control group. RT-PCR showed that the relative mRNA expressions of TNF-α and IL-6 were significantly decreased in
Amuc_1100 group compared with the control group (P<0.05), and the relative mRNA expressions of IL-10, Col1A1 and Col3A1
were significantly increased compared with the control group (P<0.05). Conclusion　Amuc_1100 can improve the wound healing rate of diabetic rats, and the mechanism may be related to reducing local inflammatory response and increasing collagen fiber
synthesis.

Key words:

Collagen fiber