Objective　 To evaluate the application effect of hospital drug delivery box （incubator） in short-term transportation of botulinum toxin type A drugs in hospital. Methods　Botulinum toxin type A was transported in an incubator from the 1st to the 15th of each month from September to December 2021， and was set as the intervention group. From the 16th to the 30th of each month， the drug was transported in ordinary cartons and was set as the control group. The effects of two different transport vehicles on maintaining the temperature stability of botulinum toxin type A drugs in the box， the occurrence of the average rate of drug package damage and the use satisfaction of nursing staff were all evaluated. Results　The intervention group was significantly better than the control group in the above three aspects， the difference was statistically significant （P<0.05）. Conclusion　The hospital drug delivery box can meet the temperature control demand of special drugs requiring cold chain transportation in the hospital， and the stability is good， can reduce or avoid drug damage， and improve the satisfaction of nursing staff.