Objective To design and fabrication of the 3D scaffolds that exhibit biomimetic multi-scale structures composed ofmicroporous structure with nanofibers for urethral tissue.Methods By analyzing themulti-scale structures of urethral acellularmatrix,a bilayer scaffold composed ofmicroporous section and nanofibrous dense section to biomimetic urethral tissue was designed.Template biosynthesis was used to fabricate 3D microporous nanofibrous gelatin/BC bilayer scaffolds(BC-Gel/BC)by stationary cultivation usingmicroporous gelatin scaffold as a template.Results The BC-Gel/BC bilayer scaffold composed of dense section of BC nanofibrils network and microporous section of Gel/BC.Themicroporous section with highly interconnected micropore(167±56)μm and surface decorated on themicropore wall by BC nanofibers (20-40)nm were fabricated,which were remarkably similar structure to the native extracellularmatrix(ECM).Conclusion Using the template biosynthesis method,the BC-Gel/BC bilayer scaffold for urethral tissue engineering can be successfully constructed,which has the bilayer structure,m icro porous and nanofibrous network to biom imetic urethral tissue.
LI Zhe,LV Xiangguo,WANG Baoxiu,FENG Chao,CHEN Shiyan,YAN Zhiyong,WANG Huaping.
. Design and Fabrication of Abacterial Cellulose Bilayer Scaffold for Urethral Tissue Engineering[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2016
, 12(5)
: 276
-280
.
DOI: 10.3969/j.issn.1673-0364.a448