Journal of Tissue Engineering and Reconstructive Surgery ›› 2016, Vol. 12 ›› Issue (5): 276-280.doi: 10.3969/j.issn.1673-0364.a448

• Original article • Previous Articles     Next Articles

Design and Fabrication of Abacterial Cellulose Bilayer Scaffold for Urethral Tissue Engineering

LI Zhe1,2,LV Xiangguo3,WANG Baoxiu1,FENG Chao3,CHEN Shiyan1,YAN Zhiyong2,WANG Huaping1.   

  1. 1 State Key Laboratory for Modification of Chem ical Fibers and Polymer Materials,College of Materials Science and Engineering,Donghua University, Shanghai 201620,China;2 Materials and Textile Engineering College,Jiaxing University,Jiaxing 314001,China;3 Department of Urology,Affiliated Sixth Peop le's Hospital,Shanghai Jiaotong University,Shanghai 201620,China. Corresponding author: WANG Huaping
  • Published:2020-07-23
  • Contact: 博士创新基金(CUSF-DH-D-2015029);国家自然科学基金(51573024)。

Abstract: Objective To design and fabrication of the 3D scaffolds that exhibit biomimetic multi-scale structures composed ofmicroporous structure with nanofibers for urethral tissue.Methods By analyzing themulti-scale structures of urethral acellularmatrix,a bilayer scaffold composed ofmicroporous section and nanofibrous dense section to biomimetic urethral tissue was designed.Template biosynthesis was used to fabricate 3D microporous nanofibrous gelatin/BC bilayer scaffolds(BC-Gel/BC)by stationary cultivation usingmicroporous gelatin scaffold as a template.Results The BC-Gel/BC bilayer scaffold composed of dense section of BC nanofibrils network and microporous section of Gel/BC.Themicroporous section with highly interconnected micropore(167±56)μm and surface decorated on themicropore wall by BC nanofibers (20-40)nm were fabricated,which were remarkably similar structure to the native extracellularmatrix(ECM).Conclusion Using the template biosynthesis method,the BC-Gel/BC bilayer scaffold for urethral tissue engineering can be successfully constructed,which has the bilayer structure,m icro porous and nanofibrous network to biom imetic urethral tissue.

Key words: Urethral tissue engineering, Acellularmatrix, Bacterial cellulose, Multi-scale structure

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