Objective To investigate the effect of repairing canine segmentalmandibular bone defects by using implant combining with bone tissue engineering.M ethods A 3 cm segmentalmandibular defect was created,and the defect was repaired with cell-scaffold constructs formed by in vitro expanded and osteogenically induced BMSCs(P2)and coral. Implantswere embedded into themandible 32 weeks post-operation(n=3);the samewas disposed for the adjacentmandible as control(n=3).Imaging,gross view,histological and biomechanicalmethodswere taken to detect the repairing effects of the bone defect at 4 weeks,12 weeks,26 weeks respectively after implantation.Results A well implant-osseous healing was identified in both experimental group and control group by X-ray and computed tomography(CT)26 weeks after implantation,while the experimental group showed a higher neogenetic bone density around the implant.Micro-CT indicated no significant density difference between the experimental group and the control group(P＞0.05).Closely connection were formed between the implant and the normal bone as well as the tissue engineered bone.Histologically,lots ofmature bone trabecula was showed in both groups.No significant mechanical-strength difference was found in mandible between thebiomechanical testing group and the normal group(P＞0.05).Conclusion The tissue-engineered bone formed by autologous osteogenic induction of BMSCs and coral can be a favorable remediation of the caninemandible.Additionally,the implanted implant can further promote bonematuration.