Journal of Tissue Engineering and Reconstructive Surgery ›› 2016, Vol. 12 ›› Issue (5): 285-288.doi: 10.3969/j.issn.1673-0364.a450

• Original article • Previous Articles     Next Articles

Research of Repairing Canine Segmental M andibular Bone Defects by Using Im p lant Combining w ith Bone Tissue Engineering

LIDong1,2,MAO Haoli3,BAIShanshan1,YUAN Jie1,WEIMin1,ZHANGWenjie1,2,LIUWei1,2,CAO Yilin1,2   

  1. 1 Department of Plastic and Reconstructive Surgery;2 Shanghai Key Laboratory of Tissue Engineering;3 Department of Anesthesiology,Shanghai Ninth People's Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200011, China.Corresponding author:YUAN Jie;ZHANGWenjie
  • Published:2020-07-23
  • Contact: 国家自然科学基金(81471886);上海市科委重点项目(134119a2000)。

Abstract: Objective To investigate the effect of repairing canine segmentalmandibular bone defects by using implant combining with bone tissue engineering.M ethods A 3 cm segmentalmandibular defect was created,and the defect was repaired with cell-scaffold constructs formed by in vitro expanded and osteogenically induced BMSCs(P2)and coral. Implantswere embedded into themandible 32 weeks post-operation(n=3);the samewas disposed for the adjacentmandible as control(n=3).Imaging,gross view,histological and biomechanicalmethodswere taken to detect the repairing effects of the bone defect at 4 weeks,12 weeks,26 weeks respectively after implantation.Results A well implant-osseous healing was identified in both experimental group and control group by X-ray and computed tomography(CT)26 weeks after implantation,while the experimental group showed a higher neogenetic bone density around the implant.Micro-CT indicated no significant density difference between the experimental group and the control group(P>0.05).Closely connection were formed between the implant and the normal bone as well as the tissue engineered bone.Histologically,lots ofmature bone trabecula was showed in both groups.No significant mechanical-strength difference was found in mandible between thebiomechanical testing group and the normal group(P>0.05).Conclusion The tissue-engineered bone formed by autologous osteogenic induction of BMSCs and coral can be a favorable remediation of the caninemandible.Additionally,the implanted implant can further promote bonematuration.

Key words: Implant, Mandibular reparation, Bonemarrow stromal cells, Coral

CLC Number: