Original article

Clonal Analysis on Low Density Culture of Keloid Fibroblasts

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  • Department of Plastic and Reconstructive Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiaotong University School of Medicine,Shanghai Key Laboratory of Tissue Engineering;

Online published: 2020-07-23

Abstract

Objective To compare the clone forming capacity between keloid fibroblasts (KFs) and normal dermal fibroblasts (NFs), and to explore whether there are pathological stem cells in keloid and the role of keloid stem cells on the mechanism of keloid formation. Methods Primary cells were obtained from keloid tissue and normal dermis respectively through enzyme digestion method, and then were cultured under low-density conditions of 4 000/dish. Two weeks later, the clone formation and morphology were determined. Results Under low-density culture conditions, clones were formed in both KFs and NFs. KFs formed distinct and dense clones while NFs formed loose and fewer ones. KFs exhibited higher clone forming efficiency (CFE) of (0.80±0.21)%than NFs with CFE of (0.18±0.06)% (P<0.05). Conclusion With low-density culture, keloid fibroblasts possess higher level of cloning capacity than normal dermal fibroblasts, which suggests pathological stem cells may exist in keloid tissues.

Cite this article

QU Miao,WU Xiaoli,CHAI Gang . Clonal Analysis on Low Density Culture of Keloid Fibroblasts[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2014 , 10(2) : 82 -99 . DOI: 10.3969/j.issn.1673-0364.2014.02.005

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