Journal of Tissue Engineering and Reconstructive Surgery >

2009 , Vol. 5 >Issue 1: 4 - 48

DOI: https://doi.org/10.3969/j.issn.1673-0364.2009.01.002

Original article

The Effects of Long-term Culture In Vitro on the Osteogenic Differentiation Potential of Human Umbilical Cord Blood Derived Mesenchymal Stem Cells

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  • 1 Shanghai Tissue Engineering Research and Development Center;Shanghai 200235;China. 2 Tissue Engineering Key Laboratory;Shanghai Ninth People′s Hospital;Shanghai Jiaotong University School of Medicine;Shanghai 200011;China.;

Online published: 2020-07-23

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Abstract

Objective To observe the effects of long-term culture in vitro on the osteogenic differentiation potential of human umbilical cord blood derived mesenchymal stem cells (UCB-MSCs). Methods The CD markers of UCB-MSCs were identified by flow cytometric analysis. Osteogenic differentiation of UCB -MSCs at different passages was evaluated by Alizarin Red staining, and measurement of alkaline phosphatase activity, osteocalcin content, and calcium content respectively. Results The CD marker expression of UCB-MSCs ...

Key words： Human umbilical cord blood derived mesenchymal stem cells; Tissue engineering; Osteogenic differentiation; Long-term culture in vitro

Cite this article

LIU Guangpeng,ZHANG Wenjie,LI Yulin,SUN Jian,ZHOU Heng,CUI Lei . The Effects of Long-term Culture In Vitro on the Osteogenic Differentiation Potential of Human Umbilical Cord Blood Derived Mesenchymal Stem Cells[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2009 , 5(1) : 4 -48 . DOI: 10.3969/j.issn.1673-0364.2009.01.002

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