The Effects of Long-term Culture In Vitro on the Osteogenic Differentiation Potential of Human Umbilical Cord Blood Derived Mesenchymal Stem Cells

doi:10.3969/j.issn.1673-0364.2009.01.002

Journal of Tissue Engineering and Reconstructive Surgery ›› 2009, Vol. 5 ›› Issue (1): 4-48.doi: 10.3969/j.issn.1673-0364.2009.01.002

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The Effects of Long-term Culture In Vitro on the Osteogenic Differentiation Potential of Human Umbilical Cord Blood Derived Mesenchymal Stem Cells

LIU Guangpeng¹,ZHANG Wenjie²,LI Yulin¹,SUN Jian¹,ZHOU Heng¹,CUI Lei¹²

1 Shanghai Tissue Engineering Research and Development Center;Shanghai 200235;China. 2 Tissue Engineering Key Laboratory;Shanghai Ninth People′s Hospital;Shanghai Jiaotong University School of Medicine;Shanghai 200011;China.;

Published:2020-07-23
Contact: 国家自然科学基金(30800232) 上海市科技启明星计划项目(07QA14053) 上海-英格兰东北地区科技合作计划(075407072)

Abstract

Abstract: Objective To observe the effects of long-term culture in vitro on the osteogenic differentiation potential of human umbilical cord blood derived mesenchymal stem cells (UCB-MSCs). Methods The CD markers of UCB-MSCs were identified by flow cytometric analysis. Osteogenic differentiation of UCB -MSCs at different passages was evaluated by Alizarin Red staining, and measurement of alkaline phosphatase activity, osteocalcin content, and calcium content respectively. Results The CD marker expression of UCB-MSCs ...

Key words: Human umbilical cord blood derived mesenchymal stem cells, Tissue engineering, Osteogenic differentiation, Long-term culture in vitro

CLC Number:

Q813.1+1

LIU Guangpeng,ZHANG Wenjie,LI Yulin,SUN Jian,ZHOU Heng,CUI Lei. The Effects of Long-term Culture In Vitro on the Osteogenic Differentiation Potential of Human Umbilical Cord Blood Derived Mesenchymal Stem Cells[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2009, 5(1): 4-48.

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The Effects of Long-term Culture In Vitro on the Osteogenic Differentiation Potential of Human Umbilical Cord Blood Derived Mesenchymal Stem Cells

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