Journal of Tissue Engineering and Reconstructive Surgery >

2009 , Vol. 5 >Issue 1: 21 - 24

DOI: https://doi.org/10.3969/j.issn.1673-0364.2009.01.006

Original article

Effects on Recombinant NF1 Gene on Collagen Expression of Hypertrophic Scar Fibroblasts In Vitro

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  • Department of Plastic Surgery;Shanghai Sixth People′s Hospital;Shanghai Jiaotong University School of Medicine;Shanghai 200233;China.;

Online published: 2020-07-23

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Abstract

Objective To explore the feasibility of transfecting recombinant NF1 into hypertrophic scar fibroblasts and investigate the proliferation and collagen Ⅰ,Ⅲ synthesis of transfected cells. Methods Recombinant human NF1 gene was transfected into hypertrophic scar fibroblasts with the karyocyte expressive vector. The expression of NF1 gene, collagen Ⅰ, Ⅲ mRNA was quantified by real time PCR. The changes of cell proliferation were observed with CCK-8 colorimeter. Results Transfected hypertrophic scar fibroblasts...

Key words： Nuclear factor-1 （NF1）; Transfection; Hypertrophic scar; Fibroblast

Cite this article

ZHENG Jianghong,DENG Chenliang,DING Zhi,MAO GuangyuYANG Songlin . Effects on Recombinant NF1 Gene on Collagen Expression of Hypertrophic Scar Fibroblasts In Vitro[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2009 , 5(1) : 21 -24 . DOI: 10.3969/j.issn.1673-0364.2009.01.006

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