Effects on Recombinant NF1 Gene on Collagen Expression of Hypertrophic Scar Fibroblasts In Vitro

doi:10.3969/j.issn.1673-0364.2009.01.006

Abstract

Abstract: Objective To explore the feasibility of transfecting recombinant NF1 into hypertrophic scar fibroblasts and investigate the proliferation and collagen Ⅰ,Ⅲ synthesis of transfected cells. Methods Recombinant human NF1 gene was transfected into hypertrophic scar fibroblasts with the karyocyte expressive vector. The expression of NF1 gene, collagen Ⅰ, Ⅲ mRNA was quantified by real time PCR. The changes of cell proliferation were observed with CCK-8 colorimeter. Results Transfected hypertrophic scar fibroblasts...

Key words: Nuclear factor-1 （NF1）, Transfection, Hypertrophic scar, Fibroblast

CLC Number:

Q753

ZHENG Jianghong,DENG Chenliang,DING Zhi,MAO GuangyuYANG Songlin. Effects on Recombinant NF1 Gene on Collagen Expression of Hypertrophic Scar Fibroblasts In Vitro[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2009, 5(1): 21-24.

[1]	YANG Yan, SHANG Ying, LYU Qiang, et al. Treatment of rabbit ear hypertrophic scar by CO2 fractional laser-assisted silk nanofiber hydrogel-loaded asiaticoside　 [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2022, 18(3): 227-.
[2]	SU Zhiguo, FAN Jincai, LIU Liqiang, et al（）. The application of enzyme digestion without filtration in the isolation of fibroblasts　 [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2022, 18(2): 103-.
[3]	DU Lin, LU Wenli, ZHANG Xiaoxu, et al（. Preparation and biological properties of double crosslinked high strength silk fibroin hydrogel [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2022, 18(2): 108-.
[4]	XU Jia, FAN Jiajun, HAN Dong, et al. Research progress on pressure therapy of hypertrophic scar [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2022, 18(2): 176-.
[5]	ZHANG Juanjuan, ZHANG Wanyu, HAN Xiaowei, XIAO Yuan, LU Wenli, LÜ Sheng. Craniofacial features with disproportionate short stature and acanthosis nigricans caused by a R248C mutation in FGFR3: A case report and literature review [J]. Journal of Diagnostics Concepts & Practice, 2020, 19(02): 129-134.
[6]	YAN Bingqian,WANG Huijing,AI Xuefeng,GONG Yiqi,TAN Yao,XU Xu,WANG Wei,FU Wei. Preliminary Research of Liposome in modRNA Transfection to Different Cell Types [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2019, 15(6): 373-383.
[7]	YU Meichan,CHEN Kaiyan,LIU Siyuan. Effect of mi R-2053 on Fibroblasts in Oral Mucosal Repair and Its Mechanism [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2019, 15(5): 325-329.
[8]	PEI Yunkun,CHENG Liying,ZHANG Yuguang. Research Progress on Tension-Related Therapy of Scar [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2019, 15(4): 266-274.
[9]	XIAO Xian,HUANG Yao,NI Pengwen,XIE Ting. Effect of Glycated bFGF on Angiogenesis of HUVEC in Vitro [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2019, 15(3): 131-136.
[10]	WANG Zi,SUN Tingye,TAO Xiaoting,WANG Xin,ZHUANG Meiting,LI Haizhou,LI Qingfeng. Weighted Genes Co-Expression Network Analysis of M2 Macrophages Regulating Hypertrophic Scar F ormation [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2019, 15(3): 146-151.
[11]	TAN Pohching,ZHOU Shuangbai,LI Qingfeng. The Effect of Subpopulations of Dermal Skin Fibroblastsin in Skin Regeneration and Maintaining Homeostasis [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2019, 15(1): 49-51.
[12]	WEI Jian, GAO Pingjin, HAN Weiqing. Effect of α7 nicotinic acetylcholine receptor activation on transforming growth factor β1-induced phenotypic transformation of adventitia fibroblasts studied in vitro [J]. Journal of Diagnostics Concepts & Practice, 2019, 18(1): 56-60.
[13]	MA Li,JIANG Sijing,JIA Daping,ZHAO Yu. Improve ment of the Survival of Human Autologous Fat Transplantation with Chitosan Sustained Release FGF2 Gene Carrier [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2018, 14(5): 253-257.
[14]	CAI Haifeng,YIN Jiafei,WANG Chen,ZHOU Renpeng,WANG Zejian,WANG Danru. The Inhibition Effect of AMPK Agonist on the Formation of Keloid [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2018, 14(4): 188-193.
[15]	GUO Zhenghong,ZHANG Zheng,ZHANG Yixin,Cosmetic Laser Center. Advances of Laser Techniques in the Treatment of Pathological Scars [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2018, 14(3): 168-173.

Effects on Recombinant NF1 Gene on Collagen Expression of Hypertrophic Scar Fibroblasts In Vitro

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments