Journal of Tissue Engineering and Reconstructive Surgery >

2009 , Vol. 5 >Issue 2: 70 - 74

DOI: https://doi.org/10.3969/j.issn.1673-0364.2009.04.003

Original article

An Experimental Study of Bone Mesenchymal Stem Cells Seeds into Small Intestinal Submucosa Induced Toward Bone In Vitro

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  • 1 Department of Orthopedic Surgery;Taian Center Hospital;Shandong;271000;China;2 Department of Orthopedic Surgery;Shanghai Sixth People s Hospital;Shanghai Jiaotong University School of Medicine;Shanghai 200233;

Online published: 2020-07-23

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Abstract

Objective To investigate the feasibility of bone mesenchymal stem cells(BMSCs) seeded into small intestinal submucosa(SIS) to form the tissue engineered bone through inducing culture.Methods The BMSCs were isolated by percoll gradient centrifugation from rabbit s bone marrow.The BMSCs were induced to become osteogenic cells in the defined medium,and were seeded on SIS as the scaffold material for the formation of cell-SIS composite.The scaffold-cell constructs were cultured in vitro for 1 week and were impl...

Key words： Tissue engineering; Small intestinal submucosa; Bone; Scaffold; Bone mesenchymal stem cells

Cite this article

ZHANG Kaigang,ZENG Bingfang,ZHANG Changqing . An Experimental Study of Bone Mesenchymal Stem Cells Seeds into Small Intestinal Submucosa Induced Toward Bone In Vitro[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2009 , 5(2) : 70 -74 . DOI: 10.3969/j.issn.1673-0364.2009.04.003

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