Journal of Tissue Engineering and Reconstructive Surgery >

2009 , Vol. 5 >Issue 4: 181 - 185

DOI: https://doi.org/10.3969/j.issn.1673-0364.2009.08.001

Original article

Effects of Media on the Proliferation and Differentiation of Bone Marrow Stromal Cells in Beagle Dogs

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  • School of Stomatology;Tissue Engineering Research Center of Stomatology;Fujian Medical University;Fuzhou 350004;China.;

Online published: 2020-07-23

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Abstract

Objective To investigate the effects of different media: α-MEM, DMEM-LG and RPMI 1640 on the biological behavior of bone marrow stromal cells (BMSCs) in Beagle dogs, and provide the more ideal culture medium for BMSCs. Methods BMSCs derived from 6 Beagle dogs were harvested and grown in α-MEM, DMEM-LG and RPMI 1640 media respectively. The proliferation, attachment rate and mineralized nodules of the cells were observed and compared. Results Cells cultured by α-MEM showed the highest attachment rat. After el...

Key words： Bone marrow stromal cells; Tissue engineering; Media; Proliferation; Differentiation

Cite this article

HUANG Yongling,YAN Fuhua,ZHONG Quan,ZHAO Xin . Effects of Media on the Proliferation and Differentiation of Bone Marrow Stromal Cells in Beagle Dogs[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2009 , 5(4) : 181 -185 . DOI: 10.3969/j.issn.1673-0364.2009.08.001

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