Journal of Tissue Engineering and Reconstructive Surgery >

2008 , Vol. 4 >Issue 6: 315 - 319

DOI: https://doi.org/10.3969/j.issn.1673-0364.2008.06.005

Original article

The Primary Study on Labeling of Mouse Embryonic Stem Cells with Quantum Dots

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  • Department of Plastic and Reconstructive Surgery;Shanghai Ninth People s Hospital;Shanghai Jiaotong University School of Medicine;National Tissue Engineering Center;Shanghai 200011;China.;

Online published: 2020-07-23

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Abstract

Objective The aim of this study is to investigate the feasibility of labeling embryonic stem cells with quantum dots (Qdots) for long-term tracking in vitro. Methods Mouse embryonic stem cells (ES) and mouse embryonic fibroblasts (MEF) were labeled with Qtracker 655. After labeling, intra-cellular Qdots was detected by fluorescence microscope and flow cytometry. The inhibition of cell proliferation was carried out by mitomycin C or colchicines treatment after cell labeling, and the inhibiting of multi-drug ...

Key words： Cell labeling; Embryonic stem cells; Quantum dots

Cite this article

PI Qingmeng,FU Wei,SHI Lungang,CAO Yilin,ZHANG Wenjie . The Primary Study on Labeling of Mouse Embryonic Stem Cells with Quantum Dots[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2008 , 4(6) : 315 -319 . DOI: 10.3969/j.issn.1673-0364.2008.06.005

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