Journal of Tissue Engineering and Reconstructive Surgery ›› 2008, Vol. 4 ›› Issue (6): 315-319.doi: 10.3969/j.issn.1673-0364.2008.06.005

• Original article • Previous Articles     Next Articles

The Primary Study on Labeling of Mouse Embryonic Stem Cells with Quantum Dots

PI Qingmeng,FU Wei,SHI Lungang,CAO Yilin,ZHANG Wenjie   

  1. Department of Plastic and Reconstructive Surgery;Shanghai Ninth People s Hospital;Shanghai Jiaotong University School of Medicine;National Tissue Engineering Center;Shanghai 200011;China.;
  • Published:2020-07-23
  • Contact: 国家重点基础研究发展计划(2005CB522705);;国家自然科学基金(30571925,30671051)。

Abstract: Objective The aim of this study is to investigate the feasibility of labeling embryonic stem cells with quantum dots (Qdots) for long-term tracking in vitro. Methods Mouse embryonic stem cells (ES) and mouse embryonic fibroblasts (MEF) were labeled with Qtracker 655. After labeling, intra-cellular Qdots was detected by fluorescence microscope and flow cytometry. The inhibition of cell proliferation was carried out by mitomycin C or colchicines treatment after cell labeling, and the inhibiting of multi-drug ...

Key words: Cell labeling, Embryonic stem cells, Quantum dots

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