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25 February 2021, Volume 16 Issue 01 Previous Issue    Next Issue

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The result analysis and clinical significance of single urine test and renal function examination in 1 200 adults of the Bai ethnic group GAO Chenni, DUAN Yuhua, JIA Liangqin, CHEN Xiaonong 2021, 16 (01):  18-21.  DOI: 10.16138/j.1673-6087.2021.01.005 Abstract ( 139 )   HTML ( 4 )   PDF (431KB) ( 43 )   Objective To identify the abnormal urine test and renal function of Bai ethnic adults in Yunnan Province by analyzing their physical examination results and provide epidemiological evidence for the prevention and treatment of chronic kidney disease（CKD） in South Western China. Methods The results of single urine test and renal function of the participants receiving medical examination from January to December 2019 in the Center of Medical Examination of the People’s Hospital of Jianchuan were collected retrospectively. The CKD Epidemiology Collaboration (CKD-EPI) formula was used to calculate glomerular filtration rate(GFR) and the results were compared and analyzed between different gender and age groups. Results A total of 1 200 Bai ethnic participants were enrolled in our study and the average age was (41.64±11.55) years. 716(59.7%) cases were male and 366 cases in male (30.5%) were abnormal in the urine test, including 300 (25.0%) cases of microscopic hematuria and 113(9.4%) cases of proteinuria. The female participants had a higher prevalence of microscopic hematuria(35.9% vs 17.6%, χ2=51.88, P<0.001) than male, while the male participants had a higher prevalence of proteinuria(11.7% vs 6.0%, χ2=11.15, P<0.001). In renal function, the GFR in 87.3% of the participants was superior to 90 mL/(min·1.73㎡), only 6(0.5%) participates’ GFR were inferior to 60 mL/(min·1.73㎡). GFR decreased gradually with age. Conclusions Physical examination is an important way to screen renal diseases. The rate of abnormal urine test and renal function was high in Bai ethnic adults in Yunnan Province and it should attract the attention of clinicians. Figures and Tables | References | Related Articles | Metrics

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Risk factors of acute kidney injury in the patients with bloodstream infection caused by different pathogens WANG Xuejie, CHEN Zijin, DU Wen, GU Feifei, YU Haijin, ZHANG Wen, CHEN Xiaonong 2021, 16 (01):  22-26.  DOI: 10.16138/j.1673-6087.2021.01.006 Abstract ( 199 )   HTML ( 3 )   PDF (515KB) ( 45 )   Objective To investigate the incidence, risk factors and prognosis of acute kidney injury(AKI) in the patients with bloodstream infection caused by different pathogens. Methods From January 1, 2018 to December 31, 2018, the patients with blood stream infection in our hospital were enrolled and four most common pathogens including Klebsiella pneumoniae, Escherichia coli, coagulase-negative Staphylococcus and Staphylococcus aureus were tested and total 348 patients were included in this study. The basic information, biochemical indicators during hospitalization and the time and type of invasive procedures before positive blood culture of patients were collected. In accordance with Kidney Disease: Improving Global Outcomes(KDIGO) AKI diagnostic criteria, the definition of AKI was an creatinine increase ≥26.5 μmol/L within 48 h or an 1.5 time increase than the baseline creatinine level within 1 week. The Logistic regression analysis was used to analyze the risk factors, incidence, and prognosis of AKI in the patients with bloodstream infection caused by different pathogens. Results A total of 348 patients with positive blood culture were included in this study. Among them, 70 patients (20.1%) developed AKI. The incidence of AKI was 23.3%, 22.2%, 22% and 15.1% in the patients infected with Staphylococcus aureus, coagulase negative Staphylococcus, Klebsiella pneumoniae and Escherichia coli, respectively. The basic creatinine in the patients with AKI was (80.01±37.09) μmol/L, which was significantly higher than that in the cases without AKI [(69.98±46.40) μmol/L, P=0.008], and the patients in the AKI group received more invasive procedures (70.0% vs 34.5%, P=0.001). Multivariate Logistic regression analysis showed that the invasive procedures were risk factors for AKI patients with bloodstream infection caused by different pathogens [odds ratio(OR)=3.25, 95% confidence interval (CI): 1.80-5.89, P<0.001]. The incidence of AKI (χ2=2.42, P=0.49), kidney function recovery (χ2=6.40, P=0.09) and mortality (χ2=2.40, P=0.49) in bloodstream infection caused by different pathogens didn’t show statistical difference. Conclusions The invasive procedures are risk factors for AKI in the patients with bloodstream infection caused by different pathogens. There is no statistical difference in the incidence and prognosis of AKI among the patients with bloodstream infection caused by different pathogens. Figures and Tables | References | Related Articles | Metrics

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Study on the relationship between vitamin D deficiency and Hashimoto’s thyroiditis FU Jiawen, LI Xiaohua, ZHANG Hongli, XU Yanhong, ZHU Yi 2021, 16 (01):  27-31.  DOI: 10.16138/j.1673-6087.2021.01.007 Abstract ( 418 )   HTML ( 5 )   PDF (567KB) ( 216 )   Objective To evaluate the relationship between Hashimoto’s thyroiditis (HT) and 25-hydroxy vitamin D. Methods A total of 334 HT patients were enrolled from the endocrine department of our hospital from January 2017 to December 2019, and 300 non-HT patients were selected as the control. There was no significant difference in gender and age composition between the two groups(P>0.05). The indexes of liver function, renal function, blood glucose, blood lipid, electrolyte level, thyroid function, thyroid antibody and vitamin D level of the two groups were collected and analyzed. Results Compared with the control group, there was no significant difference in vitamin D level and vitamin D deficiency rate between the HT group and the control group (P>0.05), while the thyroid stimulating hormone (TSH) level and the incidence of subclinical hypothyroidism in the HT group was significantly higher than that in the control group(P<0.05). The vitamin D level of female HT patients was lower and the cases of subclinical hypothyroidism were more(P<0.05). The HT patients with vitamin D deficiency were more likely to show subclinical hypothyroidism than the HT patients with normal vitamin D. Serum free triiodothyronine(FT3) was a protective factor for 25-hydroxy vitamin D[odds ratio(OR)=0.958, P=0.002], serum TSH(OR=1.080, P=0.002) and female(OR=1.167, P=0.001) were risk factors for 25-hydroxy vitamin D. Conclusions The vitamin D deficiency is prevalent in the population, especially in female. Vitamin D deficiency can lead to higher TSH level and increase the incidence of subclinical hypothyroidism in HT patients. Figures and Tables | References | Related Articles | Metrics

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Clinical research of the relationship between the neurological deficit and carotid atherosclerotic plaque in patients with acute cerebral infarction SHAO Jianwei, HU Yunyun, NI Tongtian, CHEN Min, ZHOU Weijun, MAO Enqiang, CHEN Erzhen 2021, 16 (01):  32-36.  DOI: 10.16138/j.1673-6087.2021.01.008 Abstract ( 88 )   HTML ( 3 )   PDF (478KB) ( 36 )   Objective To investigate the relationship between the neurological deficit and carotid atherosclerotic plaque in patients with acute cerebral infarction(CI). Methods From August 2015 to December 2019, 1 022 patients with CI were selected from Ruijin Hospital. According to National Institute of Health stroke scale(NIHSS) score, the patients were divided into mild group(n=572) and moderate to severe group(n=450). Meanwhile, according to carotid color Doppler ultrasound, all patients were divided into non-carotid plaque group(n=365) and carotid plaque group(n=657). Furthermore, the carotid plaque group was divided into single plaque group(n=249), double plaque group(n=91) and multiple plaque group (n=317) based on the number of plaques, and according to the echo characteristics and the plaque stability, the plaque group was divided into stable plaque group (n=316) and unstable plaque group (n=341). The difference of the incidence of carotid plaque between the mild and moderate to severe group was analyzed. Results There were 71.8%(262/365) mild patients in the non-plaque group and 43.1%(283/657) mild patients in the plaque group. There was significant difference between the two groups（χ2=57.604, P<0.01）. There was significant difference in the proportion of moderate to severe patients among single plaque, double plaque and multiple plaque groups（χ2=57.604, P<0.01). The proportion of unstable plaque in the moderate to severe group（57.4%） was higher than that in the mild group(45.8%). Multivariate Logistic regression analysis showed that the possibility of NIHSS>3 in the plaque group was 3.691 times higher than that in non-plaque group. Conclusions The severity of neurological deficit in patients with acute cerebral infarction may be related to the incidence of carotid atherosclerotic plaques, the number and character of plaques. Figures and Tables | References | Related Articles | Metrics

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Degradation of PTEN mRNA by microRNA-29 family promotes survival and lymph node invasion of non-small cell lung cancer cell CHEN Chen, YIN Shanshan, GUO Jiahui, GAO Fenghou 2021, 16 (01):  37-44.  DOI: 10.16138/j.1673-6087.2021.01.009 Abstract ( 144 )   HTML ( 4 )   PDF (1032KB) ( 73 )   Objective To investigate the regulation and the underlying molecular mechanism of microRNA(miRNA/miR)-29 family on the cancer cell proliferation and invasion of human lymph-node invasive non-small cell lung cancer (NSCLC). Methods TCGA data base was used to analyze phosphatase and tension homology deleted from chromosome 10 (PTEN) and the mRNA expression between the non-lymph node invasive and lymph node invasive tissue in NSCLC patients. The PTEN protein expression in non-lymph node invasive NSCLC A549 cells and lymph node invasive NSCLC H1299 cells was detected by Western blotting. Three different online software were used to predict the miRNAs targeting PTEN mRNA. Real-time quantitative reverse transcriptase mediated (qRT-PCR) was used to detect miR-29 family expression in A549 cells and H1299 cells. The binding sites between PTEN mRNA 3’ untranslated region(UTR) and miR-29 were predicted by using database and confirmed by luciferase report assays. miR-a/b/c mimics or inhibitors were transfected to A549 or H1299 cells, and the PTEN protein expression was detected by Western blotting. miR-29 family knock-down cells were established by CRISPR cas9 technology. Cell counting kit-8(CCK-8) assay was used to detect the difference of proliferation between negative control group (NC group) and miR-29 family knock-down group, and transwell invasion chamber test was used to detect the difference in invasion. p-Akt,Akt, phosphorylation focal adhesion kinase（p-FAK）, FAK and survivn expression was detected by Western blotting. Survivin mRNA expression was measured by qRT-PCR. CCK-8 method was used to detect the cell proliferation and cell invasion was detected by transwell method. Results The PTEN mRNA expression in the non-lymph node invasive NSCLC patients was higher than that in lymph node invasive NSCLC patients by analyzing the TCGA database. The PTEN protein expression was higher in A549 cells than that in H1299 cells, while miR-29 family expression was lower in A549 cells than that in H1299 cells. The luciferase report assays confirmed that PTEN mRNA 3’UTR was the target of miR-29 family. The PTEN protein expression was decreased after the A549 cells transfected miR a/b/c mimics for 36 h, and PTEN protein expression was increased after H1299 cells transfected miR a/b/c inhibitors for 36 h. The miR-29 family were knocked down in H1299 cells by CRISPR cas9 technology and the proliferation and invasion were significantly decreased compared with NC group. Compared with NC group, the phosphorylation level of Akt, surviving mRNA and protein expression, and the phosphorylation level of FAK were all decreased after miR-29 family was knocked down. Conclusions The miR-29 family promoted the proliferation and invasion of lymph node invasive NSCLC cells by decreasing PTEN expression and abnormally activating p-Akt and p-FAK signaling pathway. Figures and Tables | References | Related Articles | Metrics