Toll样受体4参与调控脂质诱导的平滑肌细胞炎症反应的研究

doi:10.16150/j.1671-2870.2017.05.010

诊断学理论与实践 ›› 2017, Vol. 16 ›› Issue (05): 504-509.doi: 10.16150/j.1671-2870.2017.05.010

Toll样受体4参与调控脂质诱导的平滑肌细胞炎症反应的研究

王燕萍^1,2, 陈媛媛^1,2, 吴丽苹^1,2, 陈亚芬^1,2, 杨克¹, 刘艳^1,3

1.上海交通大学医学院心血管病研究所,上海 200025;
2.上海交通大学医学院附属瑞金医院心内科,上海 200025;
3.上海交通大学医学院附属第九人民医院心内科,上海 200011

收稿日期:2017-06-20 出版日期:2017-10-25 发布日期:2017-10-25
通讯作者: 刘艳 E-mail: liuyan_ivy@126.com
基金资助:
国家自然科学基金（81470547）; 上海市科委自然科学基金（15ZR1426100）

Toll-like receptor 4 regulates the inflammation induced by lipids in smooth muscle cells

WANG Yanping^1,2, CHEN Yuanyuan^1,2, WU Liping^1,2, CHEN Yafen^1,2, YANG Ke¹, LIU Yan^1,3

1. Institute of Cardiovascular Disease, Shanghai Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;
2. Department of Cardiology, Shanghai Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;
3. Department of Cardiology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China

Received:2017-06-20 Online:2017-10-25 Published:2017-10-25

摘要/Abstract

摘要： 目的：探究Toll样受体4（Toll-like receptor 4 , TLR4）调控脂质诱导平滑肌炎症反应的机制。方法：使用TLR4敲除小鼠（以野生型C57BL/6小鼠为对照）,随机分组为对照组（普通饲料）和实验组（高脂饲料喂养）,每组各6只,12周后观察动脉斑块进展情况。体外培养TLR4基因敲除小鼠（以野生型C57BL/6小鼠为对照）的原代平滑肌细胞。此外,使用TLR4特异性抗体阻断TLR4活性后,观察氧化型低密度脂蛋白（oxidized low density lipoprotein,oxLDL）刺激下炎症因子反应。结果：高脂喂养可显著促进野生型小鼠主动脉平滑肌细胞炎症因子表达,而高脂喂养TLR4基因敲除小鼠平滑肌细胞中炎症因子表达水平无明显升高;TLR4特异性抗体可抑制oxLDL诱导的炎症反应。结论：Toll样受体4通过上调平滑肌细胞中炎症因子水平参与oxLDL诱导的炎症反应。

关键词: Toll样受体4, 平滑肌细胞, 脂质, 炎症反应, 炎症因子

Abstract: Objective: To investigate the mechanism of TLR4 in mediating the oxLDL-induced inflammation in smooth muscle cells (SMCs). Methods: TLR4 knockout mice and wild type C57BL/6 mice were randomly divided into control group (normal diet) and study group (high-fat diet) with 6 mice per group. Progression of atheroma was analyzed after 12 weeks in TLR4 knockout mice and wild type C57BL/6 mice. Primary SMCs of TLR4 knockout mice were cultured in vitro, and SMCs of wild type C57BL/6 were served as controls. SMCs were treated with oxLDL to induce inflammation, then TLR4 antibody was used to block TLR4 activity, and oxLDL-induced inflammation was compared between TLR4 knockout mice and wild type C57BL/6 mice. Results: High-fat diet could promote the expression of inflammatory cytokines in wild type C57BL/6 mice, but in TLR4 knockout mice there was no significant difference between the normal and high-fat diet groups. Moreover, blocking the activity of TLR4 could inhibit the oxLDL-induced inflammation in primary SMCs. Conclusions: TLR4 plays a role in regulating the oxLDL-induced inflammation in SMCs.

Key words: Toll-Like Receptor 4, Smooth Muscle Cells, Lipid, Inflammation, Cytokines

中图分类号:

R543.5

王燕萍, 陈媛媛, 吴丽苹, 陈亚芬, 杨克, 刘艳. Toll样受体4参与调控脂质诱导的平滑肌细胞炎症反应的研究[J]. 诊断学理论与实践, 2017, 16(05): 504-509.

WANG Yanping, CHEN Yuanyuan, WU Liping, CHEN Yafen, YANG Ke, LIU Yan. Toll-like receptor 4 regulates the inflammation induced by lipids in smooth muscle cells[J]. Journal of Diagnostics Concepts & Practice, 2017, 16(05): 504-509.

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Toll样受体4参与调控脂质诱导的平滑肌细胞炎症反应的研究

Toll-like receptor 4 regulates the inflammation induced by lipids in smooth muscle cells

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