诊断学理论与实践 ›› 2023, Vol. 22 ›› Issue (01): 75-79.doi: 10.16150/j.1671-2870.2023.01.012
收稿日期:
2022-03-07
出版日期:
2023-02-25
发布日期:
2023-07-06
通讯作者:
陈慧芬
E-mail:weedeng@sina.com
Received:
2022-03-07
Online:
2023-02-25
Published:
2023-07-06
Contact:
CHEN Huifen
E-mail:weedeng@sina.com
摘要:
细菌的抗生素耐药性已成为全球公共健康的巨大威胁。替加环素是新一代的四环素类药物,是目前治疗耐碳青霉烯类革兰氏阴性菌感染的最后一道防线。然而可移动新型四环素灭活酶tet(X)直系同源物的出现,导致了高水平替加环素耐药。目前,已经发现tet(X)的同系物主要有tet(X1)、tet(X2)、tet(X3)、tet(X3.2)、tet(X4)、tet(X5)、tet(X6)和tet(X7)。该耐药基因已经传播到世界多个地区医院中相关的患者和环境中,涉及多种不同种类的细菌,其中携带tet(X3)和tet(X4)基因的耐药细菌表现出最高的耐药水平。耐药机制中,插入序列ISCR2与tet(X3)、tet(X4)、tet(X5)的水平传播密切相关,位于多种型别质粒上tet(X4)的基因遗传结构较为复杂,可位于各式各样的可移动原件上,加速了该耐药基因的播散。质粒介导的替加环素耐药性可能会进一步扩散到各种生态位和临床高危病原体中。迫切需要临床及科研工作者共同努力,来阻止该耐药基因的传播。
中图分类号:
沈平华, 陈慧芬. 新型四环素灭活酶tet(X)致替加环素耐药的机制研究进展[J]. 诊断学理论与实践, 2023, 22(01): 75-79.
SHEN Pinghua, CHEN Huifen. Advances in mechanism study on novel tetracycline-inactivating enzymes tet(X) causing emerging tigecycline resistance[J]. Journal of Diagnostics Concepts & Practice, 2023, 22(01): 75-79.
[1] |
YONG D, TOLEMAN M A, GISKE C G, et al. Characte-rization of a new metallo-beta-lactamase gene, bla(NDM-1), and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India[J]. Antimicrob Agents Chemother, 2009, 53(12):5046-5054.
doi: 10.1128/AAC.00774-09 URL |
[2] |
LIU Y Y, WANG Y, WALSH T R, et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study[J]. Lancet Infect Dis, 2016, 16(2):161-168.
doi: 10.1016/S1473-3099(15)00424-7 URL |
[3] |
DOI Y. Treatment Options for Carbapenem-resistant Gram-negative Bacterial Infections[J]. Clin Infect Dis, 2019, 69(Suppl 7):S565-S575.
doi: 10.1093/cid/ciz830 URL |
[4] |
BEABOUT K, HAMMERSTROM T G, PEREZ A M, et al. The ribosomal S10 protein is a general target for decreased tigecycline susceptibility[J]. Antimicrob Agents Chemother, 2015, 59(9):5561-5566.
doi: 10.1128/AAC.00547-15 pmid: 26124155 |
[5] |
SPEER B S, SALYERS A A. Characterization of a novel tetracycline resistance that functions only in aerobically grown Escherichia coli[J]. Antimicrob Agents Chemother, 2015, 59(9):5561-5566.
doi: 10.1128/AAC.00547-15 URL |
[6] |
HE T, WANG R, LIU D, et al. Emergence of plasmid-mediated high-level tigecycline resistance genes in animals and humans[J]. Nat Microbiol, 2019, 4(9):1450-1456.
doi: 10.1038/s41564-019-0445-2 pmid: 31133751 |
[7] |
SUN J, CHEN C, CUI C Y, et al. Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli[J]. Nat Microbiol, 2019, 4(9):1457-1464.
doi: 10.1038/s41564-019-0496-4 pmid: 31235960 |
[8] |
SPEER B S, SALYERS A A. Novel aerobic tetracycline resistance gene that chemically modifies tetracycline[J]. J Bacteriol, 1989, 171(1):148-153.
pmid: 2644186 |
[9] |
MOORE I F, HUGHES D W, WRIGHT G D. Tigecycline is modified by the flavin-dependent monooxygenase TetX[J]. Biochemistry, 2005, 44(35):11829-11835.
pmid: 16128584 |
[10] |
WHITTLE G, HUND B D, SHOEMAKER N B, et al. Characterization of the 13-kilobase ermF region of the Bacteroides conjugative transposon CTnDOT[J]. Appl Environ Microbiol, 2001, 67(8):3488-3495.
doi: 10.1128/AEM.67.8.3488-3495.2001 URL |
[11] |
YANG W, MOORE I F, KOTEVA K P, et al. TetX is a flavin-dependent monooxygenase conferring resistance to tetracycline antibiotics[J]. J Biol Chem, 2004, 279(50):52346-52352.
doi: 10.1074/jbc.M409573200 pmid: 15452119 |
[12] |
WANG L, LIU D, LV Y, et al. Novel Plasmid-Mediated tet(X5) Gene Conferring Resistance to Tigecycline, Eravacycline, and Omadacycline in a Clinical Acinetobacter baumannii Isolate[J]. Antimicrob Agents Chemother, 2019, 64(1):e0132619.
doi: 10.1128/AAC.01326-19 URL |
[13] | LI R, LIU Z, PENG K, et al. Co-occurrence of two tet(X) variants in an Empedobacter brevis of shrimp origin[J]. Antimicrob Agents Chemother, 2019, 63(12):e01636-19. |
[14] |
LIU D, ZHAI W, SONG H, et al. Identification of the novel tigecycline resistance gene tet(X6) and its variants in Myroides, Acinetobacter and Proteus of food animal origin[J]. J Antimicrob Chemother, 2020, 75(6):1428-1431.
doi: 10.1093/jac/dkaa037 pmid: 32068864 |
[15] |
GASPARRINI A J, MARKLEY J L, KUMAR H, et al. Tetracycline-inactivating enzymes from environmental, human commensal, and pathogenic bacteria cause broad-spectrum tetracycline resistance[J]. Commun Biol, 2020, 3(1):241.
doi: 10.1038/s42003-020-0966-5 pmid: 32415166 |
[16] | CUI C Y, H E Q, JIA Q L, et al. Evolutionary trajectory of the tet(X) family: critical residue changes towards high-level tigecycline resistance[J]. mSystems, 2021, 6(3):e00050-21. |
[17] |
GHOSH S, LAPARA T M. The effects of subtherapeutic antibiotic use in farm animals on the proliferation and persistence of antibiotic resistance among soil bacteria[J]. ISME J, 2007, 1(3):191-203.
doi: 10.1038/ismej.2007.31 pmid: 18043630 |
[18] |
MING D S, CHEN Q Q, CHEN X T. Analysis of resistance genes in pan-resistant Myroides odoratimimus clinical strain PR63039 using whole genome sequencing[J]. Microb Pathog, 2017, 112:164-170.
doi: 10.1016/j.micpath.2017.09.012 URL |
[19] |
LESKI T A, BANGURA U, JIMMY D H, et al. Multidrug-resistant tet(X)-containing hospital isolates in Sierra Leone[J]. Int J Antimicrob Agents, 2013, 42(1):83-86.
doi: 10.1016/j.ijantimicag.2013.04.014 URL |
[20] |
SÁRVÁRI K P, SÓKI J, KRISTÓF K, et al. Molecular characterisation of multidrug-resistant Bacteroides isolates from Hungarian clinical samples[J]. J Glob Antimicrob Resist, 2018, 13:65-69.
doi: S2213-7165(17)30207-2 pmid: 29101081 |
[21] |
ZENG J, PAN Y, YANG J, et al. Metagenomic insights into the distribution of antibiotic resistome between the gut-associated environments and the pristine environments[J]. Environ Int, 2019, 126:346-354.
doi: S0160-4120(18)33166-0 pmid: 30826613 |
[22] |
AYDIN S, INCE B, INCE O. Development of antibiotic resistance genes in microbial communities during long-term operation of anaerobic reactors in the treatment of pharmaceutical wastewater[J]. Water Res, 2015, 83:337-344.
doi: 10.1016/j.watres.2015.07.007 pmid: 26188597 |
[23] |
USUI M, FUKUDA A, SUZUKI Y, et al. Broad-host-range IncW plasmid harbouring tet(X) in Escherichia coli isolated from pigs in Japan[J]. J Glob Antimicrob Resist, 2022, 28:97-101.
doi: 10.1016/j.jgar.2021.12.012 URL |
[24] | SOLIMAN A M, RAMADAN H, ZARAD H, et al. Coproduction of Tet(X7) conferring high-level tigecycline resistance, fosfomycin FosA4, and colistin mcr-1.1 in escherichia coli strains from chickens in Egypt[J]. Antimicrob Agents Chemother, 2021, 65(6):e02084-20. |
[25] |
PARK B H, LEVY S B. The cryptic tetracycline resistance determinant on Tn4400 mediates tetracycline degradation as well as tetracycline efflux[J]. Antimicrob Agents Chemother, 1988, 32(12):1797-1800.
doi: 10.1128/AAC.32.12.1797 pmid: 3072922 |
[26] | SONG H, LIU D, LI R, et al. Polymorphism existence of mobile tigecycline resistance gene tet(X4) in escherichia coli[J]. Antimicrob Agents Chemother, 2020, 64(2):e01825-19. |
[27] |
CHEN C, CUI C Y, ZHANG Y, et al. Emergence of mobile tigecycline resistance mechanism in Escherichia coli strains from migratory birds in China[J]. Emerg Microbes Infect, 2019, 8(1):1219-1222.
doi: 10.1080/22221751.2019.1653795 URL |
[28] | FANG L X, CHEN C, YU D L, et al. Complete nucleotide sequence of a novel plasmid bearing the high-level tigecycline resistance gene tet(X4)[J]. Antimicrob Agents Chemother, 2019, 63(11):e01373-19. |
[29] |
HE T, WEI R, LI R, et al. Co-existence of tet(X4) and mcr-1 in two porcine escherichia coli isolates[J]. J Antimicrob Chemother, 2020, 75(3):764-766.
doi: 10.1093/jac/dkz510 pmid: 31840165 |
[30] |
HE Y Z, LI X P, MIAO Y Y, et al. The IS Apl1 2 dimer circular intermediate participates in mcr-1 transposition[J]. Front Microbiol, 2019, 10:15.
doi: 10.3389/fmicb.2019.00015 URL |
[31] |
TOLEMAN M A, BENNETT P M, WALSH T R. ISCR elements: novel gene-capturing systems of the 21st century?[J]. Microbiol Mol Biol Rev, 2006, 70(2):296-316.
doi: 10.1128/MMBR.00048-05 URL |
[32] |
LI R, PENG K, LI Y, et al. Exploring tet(X)-bearing tigecycline-resistant bacteria of swine farming environments[J]. Sci Total Environ, 2020, 733:139306.
doi: 10.1016/j.scitotenv.2020.139306 URL |
[33] |
CHENG Y, CHEN Y, LIU Y, et al. Detection of a new tet(X6)-encoding plasmid in Acinetobacter towneri[J]. J Glob Antimicrob Resist, 2021, 25:132-136.
doi: 10.1016/j.jgar.2021.03.004 pmid: 33762210 |
[34] | HSIEH Y C, WU J W, CHEN Y Y, et al. An outbreak of tet(X6)-carrying tigecycline-resistant acinetobacter baumannii isolates with a new capsular type at a hospital in taiwan[J]. Antibiotics (Basel), 2021, 10(10):1239. |
[35] |
ROBERTS M C. Environmental macrolide-lincosamide-streptogramin and tetracycline resistant bacteria[J]. Front Microbiol, 2011, 2:40.
doi: 10.3389/fmicb.2011.00040 pmid: 21833302 |
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