阴道分泌物分离出葡萄牙假丝酵母菌一株报告

doi:10.16150/j.1671-2870.2024.03.012

诊断学理论与实践 ›› 2024, Vol. 23 ›› Issue (03): 330-334.doi: 10.16150/j.1671-2870.2024.03.012

阴道分泌物分离出葡萄牙假丝酵母菌一株报告

刘慧¹, 卢发强²()

1.大连大学附属中山医院检验科，大连 116001
2.铜仁市人民医院检验科，铜仁 554300

收稿日期:2023-09-01 接受日期:2024-01-30 出版日期:2024-06-25 发布日期:2024-06-25
通讯作者: 卢发强 E-mail：faqianglu@yahoo.com

One strain of Candida lusitaniae isolated from vaginal secretions: a case report

LIU Hui¹, LU Faqiang²()

1. Department of Laboratory, Zhongshan Hospital Affiliated to Dalian University, Dalian 116001, China
2. Department of Laboratory, Tongren People's Hospital, Tongren 554300, China

Received:2023-09-01 Accepted:2024-01-30 Published:2024-06-25 Online:2024-06-25

摘要/Abstract

摘要：

葡萄牙假丝酵母菌性阴道炎在临床较为罕见，本文报道1例由葡萄牙假丝酵母菌（Candida lusitaniae, CL）引起外阴阴道假丝酵母菌病(vulvovaginal candidiasis, VVC)的患者。本例患者为40岁女性，主要临床表现为月经前外阴瘙痒、白带增多、反复半年。将患者阴道分泌物直接涂片分别进行免疫荧光染色、革兰染色、瑞氏染色，镜检找到真菌孢子，CHROMagar显色平板鉴定为光滑假丝酵母菌，梅里埃VITEK-2 Compact全自动微生物分析系统鉴定为CL。该病例的诊治过程提示，CL实验室诊断易与其他假丝酵母菌混淆，为防止漏报或错报，对于难治病例需要考虑少见菌株感染可能。

关键词: 菌种鉴定, 葡萄牙假丝酵母菌, 非白假丝酵母菌, 科玛嘉显色培养基, 免疫荧光染色

Abstract:

Candida lusitaniae vaginitis is clinically rare. This article reports a case of vaginitis caused by Candida lusitaniae in a 40-year-old female patient. The main clinical manifestations of the patient were pruritus vulvae before menstruation, increased vaginal discharge, and recurrent symptoms for six months. Vaginal secretions from the patient were directly smeared and subjected to immunofluorescence staining, Gram staining, and Giemsa staining, revealing fungal spores upon microscopic examination. Identification using CHROMagar chromogenic plates revealed the presence of Candida lusitaniae. The bioMérieux VITEK-2 Compact automated microbiology system identified the organism as Candida lusitaniae. The diagnostic and treatment process of this case suggests that laboratory diagnosis of Candida lusitaniae can easily be confused with other Candida species. To prevent underreporting or misreporting, it is necessary to consider the possibility of infection by rare strains in refractory cases.

Key words: Identification of bacterial strains, Portuguese Candida albicans, Non-Candida albicans yeast, Komajia color development medium, Immunofluorescence staining

中图分类号:

刘慧, 卢发强. 阴道分泌物分离出葡萄牙假丝酵母菌一株报告[J]. 诊断学理论与实践, 2024, 23(03): 330-334.

LIU Hui, LU Faqiang. One strain of Candida lusitaniae isolated from vaginal secretions: a case report[J]. Journal of Diagnostics Concepts & Practice, 2024, 23(03): 330-334.

图/表 4

参考文献 11

[1]	刘朝晖, 廖秦平. 外阴阴道假丝酵母菌病(VVC)诊治规范修订稿[C]. 西南及四川省中医妇(产)科专科联盟成立大会论文集, 2018:319-320.
	LIU Z H, LIAO Q P. Revised diagnosis and treatment guidelines for vulvovaginal candidiasis (VVC)[C]. Proceedings of the Establishment Conference of the Southwest and Sichuan Traditional Chinese Medicine Obstetrics and Gynecology Specialist Alliance, 2018:319-320.
[2]	曾文, 华丽燕, 欧阳凯, 等. 外阴阴道假丝酵母菌病病原菌菌种分布及耐药性分析[J]. 现代医院, 2018, 18(7):1055-1058.
	ZENG W, HUA L Y, OU Y K, et al. Analysis of pathogenic bacteria distribution and drug resistance of vulvovaginal candidiasis[J]. Mod Hosp, 2018, 18(07):1055-1058.
[3]	杜笠, 杜秋蓉, 刘祥琴, 等. 女性生殖道念珠菌病病原体检测方法探讨[J]. 实用医院临床杂志, 2021, 18(3):51-53.
	DU L, DU Q R, LIU X Q, et al. A preliminary study on the detection methods of vaginal candidiasis pathogen[J]. Pract J Clin Med, 2021, 18(3):51-53.
[4]	李筱芳. 外阴阴道念珠菌病的诊疗[J]. 皮肤科学通报, 2021, 38(1):65-69.
	LI X F. The management of vulvovaginal candidiasis[J]. Dermatol Bull, 2021, 38 (1): 65-69.
[5]	FARR A, EFFENDY I, FREY TIRRI B, et al. Guideline: Vulvovaginal candidosis (AWMF 015/072, level S2k)[J]. Mycoses, 2021, 64(6):583-602.
[6]	张建萍, 苏霞, 周海. 湿片法检测阴道分泌物中真菌技术改良[J]. 现代检验医学杂志, 2003, 18(4):16-16.
	ZHANG J P, SU X, ZHOU H. Improvement of wet slide method for detecting fungi in vaginal secretions[J]. J Mod Lab Med, 2003, 18(4):16-16.
[11]	DING W J, PU Z F, QU W. Comparison of accuracy of different identification methods of VVC (vulvovaginal candidiasis)-related Candida glabrata and its drug sensitivity in vitro[J]. Chin J Nosocomiol, 2018, 28 (11):1625-1628.
[12]	MULET BAYONA J V, SALVADOR GARCÍA C, TORMO PALOP N, et al. Evaluation of a novel chromogenic medium for Candida spp. identification and comparison with CHROMagar™ Candida for the detection of Candida auris in surveillance samples[J]. Diagn Microbiol Infect Dis, 2020, 98(4):115168.
[13]	PATEL R. A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification[J]. J Fungi (Basel), 2019, 5(1):4.
[14]	MAROT-LEBLOND A, NAIL-BILLAUD S, PILON F, et al. Efficient diagnosis of vulvovaginal candidiasis by use of a new rapid immunochromatography test[J]. J Clin Microbiol, 2009, 47(12):3821-3825.
[15]	OTAŠEVIĆ S, MOMČILOVIĆ S, STOJANOVIĆ N M, et al. Non-culture based assays for the detection of fungal pathogens[J]. J Mycol Med, 2018, 28(2):236-248. doi: S1156-5233(18)30007-6 pmid: 29605542
[16]	AGUIRRE-QUIÑONERO A, CASTILLO-SEDANO I S, CALVO-MURO F, et al. Accuracy of the BD MAX™ vaginal panel in the diagnosis of infectious vaginitis[J]. Eur J Clin Microbiol Infect Dis, 2019, 38(5):877-882.
[17]	ABBASI NEJAT Z, FARAHYAR S, FALAHATI M, et al. Molecular identification and antifungal susceptibility pattern of non-albicans candida species isolated from vulvovaginal candidiasis[J]. Iran Biomed J, 2017, 22(1):33-41. pmid: 28688376
[18]	SILVERMAN N S, MORGAN M, NICHOLS W S. Candida lusitaniae as an unusual cause of recurrent vaginitis and its successful treatment with intravaginal boric acid[J]. Infect Dis Obstet Gynecol, 2001, 9(4):245-247. pmid: 11916183
[19]	ANH D N, HUNG D N, TIEN T V, et al. Prevalence, species distribution and antifungal susceptibility of Candida albicans causing vaginal discharge among symptomatic non-pregnant women of reproductive age at a tertiary care hospital, Vietnam[J]. BMC Infect Dis, 2021, 21(1):523. doi: 10.1186/s12879-021-06192-7 pmid: 34082699
[20]	HASHEMI S E, SHOKOHI T, ABASTABAR M, et al. Species distribution and susceptibility profiles of Candida species isolated from vulvovaginal candidiasis, emergence of C[J]. lusitaniae, Curr Med Mycol, 2019; 5(4):26-34.
[7]	LIAO J, PAN B, LIAO G, et al. Synthesis and immunological studies of β-1,2-mannan-peptide conjugates as antifungal vaccines[J]. Eur J Med Chem, 2019, 173:250-260. doi: S0223-5234(19)30300-9 pmid: 31009911
[8]	董一红, 王丽芳, 张梦, 等. 不同方法对外阴阴道假丝酵母菌病的检出效果[J]. 国际检验医学杂志, 2018, 39(23):2869-2872.
	DONG Y H, WANG L F, ZHANG M, et al. Performance evaluation of different methods for the detection of Candida vulvovaginalis[J]. Int J Lab Med, 2018, 39(23):2869-2872.
[9]	赵磊, 殷舟, 叶佳铭. 不同检测方法诊断妇科炎症真菌感染的对比研究[J]. 中国妇幼保健, 2019, 34(24):5608-5610.
	ZHAO L, YIN Z, YE J M. Comparative study on diffe-rent detection methods for diagnosing gynecological inflammatory fungal infections[J]. Matern Child Health Care Chin, 2019, 34(24):5608-5610.
[10]	LEENA SANKARI S, MAHALAKSHMI K, NAVEEN KUMAR V. Chromogenic medium versus PCR-RFLP in the speciation of Candida: a comparative study[J]. BMC Res Notes, 2019, 12(1):681. doi: 10.1186/s13104-019-4710-5 pmid: 31640775
[11]	丁文静, 濮珍凤, 渠巍. 不同检测方法对VVC光滑假丝酵母的鉴定准确度比较及体外药物敏感性研究[J]. 中华医院感染学杂志, 2018, 28(11):1625-1628.

阴道分泌物分离出葡萄牙假丝酵母菌一株报告

One strain of Candida lusitaniae isolated from vaginal secretions: a case report

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 4

参考文献 11

相关文章 0

编辑推荐

Metrics

本文评价