多发性硬化症与EB病毒及白细胞介素17/白细胞介素23关联性分析

doi:10.16150/j.1671-2870.2017.01.017

诊断学理论与实践 ›› 2017, Vol. 16 ›› Issue (01): 88-92.doi: 10.16150/j.1671-2870.2017.01.017

多发性硬化症与EB病毒及白细胞介素17/白细胞介素23关联性分析

孟俊¹, 彭奕冰¹, 王学锋¹, 陈呢喃², 张冬青²

1.上海交通大学医学院附属瑞金医院检验科,上海 200025;
2.上海交通大学医学院上海市免疫学研究所,上海 200025

收稿日期:2016-12-01 出版日期:2017-02-25 发布日期:2022-06-20
通讯作者: 张冬青 E-mail: dqzhang1333@163.com
基金资助:
国家自然科学基金面上项目(31270963); 上海市科委重点连续资助项目（10JC1408500）; 上海交通大学医学院附属瑞金医院优青医技后备人才基金

A study on correlation between multiple sclerosis and Epstein-Barr virus and IL-17/IL-23

MENG Jun¹, PENG Yibing¹, WANG Xuefeng¹, CHEN Ninan², ZHANG Dongqing²

1. Department of Clinical Laboratory, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;
2. Shanghai Jiao Tong University School of Medicine, Shanghai Institute of Immunology, Shanghai 200025, China

Received:2016-12-01 Online:2017-02-25 Published:2022-06-20

摘要/Abstract

摘要： 目的探讨抗EB病毒（Epstein-Barr virus,EBV）抗体在多发性硬化症（multiple sclerosis,MS）患者脑脊液中的表达情况,以及细胞因子白细胞介素（interleukin,IL）-17、IL-23与其发病间的关联性,借以对MS发病机制进行研究,并为临床诊治和实验室检验提供进一步参考。方法选取29例中枢神经免疫疾病患者（研究组,包括MS、视神经脊髓炎等）和43例同期非中枢神经免疫疾病患者（对照组,包括周围神经病、偏头痛等）,采用酶联免疫吸附试验（enzyme-linked immunosorbent assay,ELISA）分别检测2组患者脑脊液中EBV-壳抗原（VCA） IgM、EBV-核抗原（EBNA）-1 IgG、IL-17、IL-23的表达水平。结果中枢神经免疫疾病患者（研究组）患者脑脊液中EBV-EBNA-1 IgG、EBV-VCA IgM和IL-23的表达水平显著高于非中枢神经免疫疾病患者（对照组）(P<0.01)。研究组中MS患者脑脊液（cerebrospinal fluid,CSF）IL-23表达水平较对照组显著增高（P<0.01）,同时MS亚组患者CSF的IL-17表达水平较对照组显著增高（P<0.05）。结论抗EBV抗体的表达预示着MS患者的发病可能与EBV感染相关,可能是MS的发病机制之一。同时,IL-17/IL-23轴的异常也可能与MS发病间存在一定的关联。MS的发病不仅与EB病毒感染有关,且与患者的免疫紊乱密切相关。

关键词: 脑脊液, 多发性硬化, EB病毒抗体, 白细胞介素, 酶联免疫吸附试验

Abstract: Objective: To study the expression of Epstein-Barr virus (EBV) autoantibodies and IL-17, IL-23 in cerebrospinal fluid of patients with multiple sclerosis (MS) and to define the relationship between IL-17/IL-23 and pathogenesis of MS for hoping to provide a diagnostic reference for clinical practice. Methods: Seventy-two patients with neurological diseases in Ruijin Hospital were categorized into 2 groups: 29 cases with central nervous system autoimmune disease (study group) and 43 cases of non-central nervous system autoimmune disease (control group). Enzyme-linked immunosorbent assay (ELISA) was used to assess the levels of EBV-VCA-IgM, EBV-EBNA-1IgG, IL-17, IL-23 in CSF. Results: The expressions of EBV-EBNA-1IgG, EBV-VCA IgM and IL-23 in study group were significantly higher than those in control group (P<0.01). In study group, the expression of IL-23 in MS patients was significantly higher than that in control group（P<0.01), and the expression of IL-17 in MS patients was higher than that in control group（P<0.05）. Conclusions: EBV is related to MS and probably contributes to the pathogenesis of MS. Meanwhile, the abnormal inflammatory axis of IL-17/IL-23 may also be related to the induction of MS. The study indicates a relationship of MS not only with viral infection, but also with a disordered autoimmune system.

Key words: Cerebrospinal fluid, Multiple Sclerosis, EBV autoantibodies, Interleukin, Enzyme-linked immunosorbent assay

中图分类号:

R593.2

孟俊, 彭奕冰, 王学锋, 陈呢喃, 张冬青. 多发性硬化症与EB病毒及白细胞介素17/白细胞介素23关联性分析[J]. 诊断学理论与实践, 2017, 16(01): 88-92.

MENG Jun, PENG Yibing, WANG Xuefeng, CHEN Ninan, ZHANG Dongqing. A study on correlation between multiple sclerosis and Epstein-Barr virus and IL-17/IL-23[J]. Journal of Diagnostics Concepts & Practice, 2017, 16(01): 88-92.

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多发性硬化症与EB病毒及白细胞介素17/白细胞介素23关联性分析

A study on correlation between multiple sclerosis and Epstein-Barr virus and IL-17/IL-23

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