Induction of fluconazole resistance in Candida glabrata and Candida parapsilosis in vitro and corresponding mechanism

doi:10.16150/j.1671-2870.2017.05.011

Journal of Diagnostics Concepts & Practice ›› 2017, Vol. 16 ›› Issue (05): 510-515.doi: 10.16150/j.1671-2870.2017.05.011

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Induction of fluconazole resistance in Candida glabrata and Candida parapsilosis in vitro and corresponding mechanism

LIU Jinyan¹, WANG Ying², LI Wenjing³, ZHAO Yue³, MENG Lingning³, ZHU Weiwei¹, SHI Ce³, XIANG Mingjie^1,3

1. Department of Clinical Laboratory, Luwan Branch, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200020, China;
2. Department of Clinical Laboratory, Southern District of Anhui Provincial Hospital, Anhui Hefei 230001, China;
3. Department of Clinical Laboratory, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Received:2017-04-27 Online:2017-10-25 Published:2017-10-25

Abstract

Abstract: Objective: To investigate the susceptibility and mechanism of resistance to fluconazole in Candida glabrata and Candida parapsilosis isolates. Methods: Four strains(one clinical C. glabrata, one clinical C. parapsilosis, ATCC2001 and ATCC22019) were induced to be resistance strains in vitro by increasing concentrations of fluconazole. The expression levels of transporter genes and their transcription factor, and azole antifungal target gene were identified by real time-PCR. Meanwhile, the sequence of PDR1 gene of C. glabrata and MRR1, ERG11 gene of C. parapsilosis were determined by PCR based DNA sequencing. Results: C. glabrata was more prone to be resistant to fluconazole than C. parapsilosis. CDR1 was significantly overexpressed in resistant strains of C. glabrata, while MDR1 was significantly overexpressed in resistant strains of C. parapsilosis. PDR1 mutations Y932C, V847F were identified in resistant strains of C.glabrata, and MRR1 mutations P295S, I799S were found in resistant strains of C. parapsilosis. Conclusions: C. glabrata and C. parapsilosis showed different susceptibility to fluconazole resistance, and difference also occurred in resistance mechanism. And the function of new identified mutations in transcription factor needs further verification.

Key words: Candida spp, Resistance of fluconazole, Efflux pump, Target enzyme, Transcription factor

CLC Number:

R519

LIU Jinyan, WANG Ying, LI Wenjing, ZHAO Yue, MENG Lingning, ZHU Weiwei, SHI Ce, XIANG Mingjie. Induction of fluconazole resistance in Candida glabrata and Candida parapsilosis in vitro and corresponding mechanism[J]. Journal of Diagnostics Concepts & Practice, 2017, 16(05): 510-515.

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Induction of fluconazole resistance in Candida glabrata and Candida parapsilosis in vitro and corresponding mechanism

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