Abstract: Objective: To establish a normal reference range for 1,5-anhydroglucitol (1,5AG) and to verify its clinical application. Methods: A total of 1 175 healthy people undergone physical examination during August 2015 to December 2017 were enrolled to establish a normal reference range and the differences in 1,5AG level between different age and sex were analyzed. According to the diabetes diagnostic criteria (WHO 1999), the subjects used for verifying the self-built refe-rence range were divided into the verified diabetes disease group (300 cases, 136 males) and the verified control group (169 cases, 75 males). When the 1,5AG level was within the self-built reference range, it would be regard as negative result, otherwise as positive result; the positive rates between the 2 verified groups were compared. Results: There were differences in 1,5AG level between different age groups, so were between male and female. For people under 30 years of age, normal reference range was 86-418 μmol/L in male, and was 37-382 μmol/L in female, and for people aged 30 to 59 years, were 79-406 μmol/L and 43-297 μmol/L, while for people older than 60 years were 86-404 μmol/L and 27-278 μmol/L. The level of 1,5AG of diabetes patients was lower than that of non-diabetic patients, and the positive rate according to the self-built reference range in diabetes patients was higher than that in non-diabetic patients (71% vs 5%）. Conclusions: A reference interval of 1,5AG is established based on the population in Shanghai area, which has gender and age differences. This study will provide a reference for future large-scale population study.

Key words: Diabetes mellitus, 1, 5-Anhydroglucitol, Reference range