Multicenter study on rpoB mutation in rifampicin-resistant Mycobacterium tuberculosis isolates of initial retreatment pulmonary tuberculosis

doi:10.16150/j.1671-2870.2018.06.003

Abstract

Abstract: Objective: To observe the association between mutation of rifampicin resistance determining regions (RRDR) of rpoB gene and rifampicin resistance in rifampicin-resistant Mycobacterium tuberculosis(Mtb) strains isolated from initial retreatment pulmonary tuberculosis. Methods: Mtb isolates from pulmonary tuberculosis patients who had previous treated were collected. Drug susceptibility was identified through BACTEC 960 and Roche medium; rpoB gene mutation was analyzed by polymerase chain reaction (PCR). Results: A total of 141 isolates from 141 patients in 19 hospitals from 12 provinces across the country were tested for drug sensitivity and analysis of RRDR loci sequencing. Point mutations were identified in 72 strains by sequencing and 71 were in RRDR. The main mutation sites included codon 531 (42 strains), 526 (11 strains) and 516 (10 strains). Seventy-five strains were resistant to rifampicin (RFP), of which 70 (93.3%) had rpoB mutation and 69 were in RRDR. Only two strains with RRDR mutation (L511P and H526N mutant Mtb) were sensitive to rifampicin, and 70 rpoB mutation strains were resistant to rifampicin. Conclusions: The rifampicin-resistance in Mtb isolates from initial retreatment pulmonary tuberculosis is associated with rpoB gene RRDR mutation. RRDR mutation detection can be used for rapid identification of rifampicin-resistant Mtb in retreated patients.

Key words: Mycobacterium tuberculosis, Rifampicin, Drug resistance, rpoB

CLC Number:

R378.91⁺1

CHENG Qijian, CHEN Ling, DU Wei, LIU Yahui, YANG Hua, XIAO Heping, SHI Guochao. Multicenter study on rpoB mutation in rifampicin-resistant Mycobacterium tuberculosis isolates of initial retreatment pulmonary tuberculosis[J]. Journal of Diagnostics Concepts & Practice, 2018, 17(06): 635-639.

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