Plasmids typing of genes carrying blaKPC-2 in Klebsiella pneumoniae isolated at a tertiary hospital in Shanghai

doi:10.16150/j.1671-2870.2018.06.004

Abstract

Abstract: Objective: To investigate the plasmids typing of genes carrying bla_KPC-2 in carbapenemase resistant Klebsiella pneumoniae for exploring its epidemiological evolution. Methods: Thirty-eight carbapenem -resistant Klebsiella pneumoniae isolates obtained consecutively from Huashan Hospital were collected, all carrying bla_KPC-2-bearing plasmids. All the strains were subjected to multilocus sequence typing (MLST). Relaxase analysis was used to classify these plasmids. Results: Eight of the bla_KPC-2-bearing plasmids belonged to the MOB_P3 and 30 bla_KPC-2-bearing plasmids belonged to the MOB_F12. Phylogenetic analysis revealed that the MOB_F12 plasmids were located at two different branches, respectively, suggesting they had different biological origin. Conclusions: It is the first time that relaxase has been used for plasmids typing of genes carrying bla_KPC-2 in carbapenemase resistant Klebsiella pneumoniae, and found 8 belonged to the MOB_P3 and 30 belonged to the MOB_F12, and the MOB_F12 having different biological origin, denoting the epidemiological evolution of bla_KPC-2 bearing plasmid in carbapenemase resistant Klebsiella pneumoniae.

Key words: Klebsiella pneumoniae, bla_KPC, Plasmid, Relaxase

CLC Number:

R446.5

SHEN Pinghua, ZHANG Xianhua, JIANG Xiaofei, CHEN Huifen. Plasmids typing of genes carrying bla_KPC-2 in Klebsiella pneumoniae isolated at a tertiary hospital in Shanghai[J]. Journal of Diagnostics Concepts & Practice, 2018, 17(06): 640-644.

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Plasmids typing of genes carrying bla_KPC-2 in Klebsiella pneumoniae isolated at a tertiary hospital in Shanghai

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