The structure of gut microbiome in idiopathic short stature profiled by 16S rRNA second generation sequencing sequencing

doi:10.16150/j.1671-2870.2021.02.006

Abstract

Abstract:

Objective: To investigate structural characteristics of gut microbiome in idiopathic short stature (ISS) children and difference in gut microbiome between ISS and healthy children(HC) children, so as to explore the possible mechanism of action of gut microbiome on ISS. Methods: A total of 16 children aging from 4 to 8, including 16 ISS children (ISS group)and 16 HC (control group) were enrolled in the study. Flora of the collected feces were collected, and V3-V4 of bacterial 16S rRNA was sequenced with high-throughput sequencing. Diversity and abundance of gut microbiome between HC and ISS group were compared. Results: The structure of gut microbiome in ISS children were significantly different from HC children. The Chao1 (diversity index) of ISS group were lower than that of HC group (1109.85±435.23 vs. 1485.68±334.51) (P=0.018) as well as Observed_species index(946.98±374.93 vs. 1 217.69±289.66)(P=0.038), indicating decreased relative abundance of gut microbiome in ISS group. It’s also showed that the relative abundances of Faecalibacterium and Eubacterium were significantly lower in ISS group than HC group (P<0.05). In ISS group, both abundances of Faecalibacterium and Eubacterium were positively correlated with height-SDS and insulin-like growth factor 1(IGF1)-SDS（r>0.6, P<0.05）. Conclusions: For ISS, the abundance of gut microbiome decreases and the flora also experiences significant structural changes. Faecalibacterium and Eubacterium may decrease IGF-1 by short-chain fatty acid (SCFA), constituting a possible pathogenic mechanism for ISS.

Key words: Idiopathic short stature, Gut microbiome, 16S rRNA

CLC Number:

R725.8

LI Lin, AN Jingjing, WANG Junqi, WANG Xinqiong, DONG Zhiya. The structure of gut microbiome in idiopathic short stature profiled by 16S rRNA second generation sequencing sequencing[J]. Journal of Diagnostics Concepts & Practice, 2021, 20(02): 149-154.

Figures/Tables 6

References 17

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参数	ISS组（n=16）	对照组(n=16)	P值
男性/女性	8/8	8/8	-
年龄（岁）	6.08±1.04	6.41±1.02	0.866
身高SDS	-2.34±0.29	0.52±0.27	<0.001
体质量指数SDS	0.04±0.74	0.81±0.75	0.007
靶身高（cm）	163.78±6.69	164.78±7.13	0.685