Expression of BRAF V600E (VE1) in thyroid papillary carcinoma and its clinical significance

doi:10.16150/j.1671-2870.2018.05.013

Abstract

Abstract: Objective: To investigate the expressions of BRAF V600E, CK19 and galectin-3 antibodies in papillary thyroid carcinoma, and study the diagnostic value of BRAF V600E antibody when in corporating with the clinicopathological characteristics of PTC. Methods: The clinicopathological data of 80 patients with PTC were analyzed; 31 cases of nodular goiter and 13 cases with Hashimoto thyroiditis were served as controls. BRAF V600E, CK19 and galectin-3 antibodies in PTC and control group were detecteds and the correlation between BRAF V600E antibody and clinicopathological characteristics of PTC was analyzed. Results: Correlation between thyroid membrane invasion and lymph node metastasis was observed in PTC patients(P<0.05). The positive rates of BRAF V600E, CK19 and galectin-3 antibodies in patients with PTC were 62.50%, 98.75%, 95.00%, respectively. The sensitivity of CK19 combined with galectin-3antibodies was 95.00%, and the specificity of BRAF V600 antibody was 100%. The specificity of CK19 and galection-3 antibodies could be improved by combining with BRAF V600 antibody. There was no statistical correlation between expression of BRAF V600E antibody and age, gender, lesion size, thyroid membrane invasion, lymph node metastasis, histological subtype of PTC (P>0.05). Conclusions: The combined detection of BRAF V600E, CK19 and Galectin-3 antibodies is helpful for the pathological diagnosis of PTC.Meanwhile, BRAF V600E antibody plays a preliminary screening role for the mutation of BRAF V600E gene.

Key words: Papillary thyroidcarcinoma, BRAF V600E antibody, Gene mutation

CLC Number:

R736.1

CAI Rong, MIN Xuewen, CHEN Meirong, SHEN Yating, SHI Qunli, ZHOU Xiaodie. Expression of BRAF V600E (VE1) in thyroid papillary carcinoma and its clinical significance[J]. Journal of Diagnostics Concepts & Practice, 2018, 17(05): 552-556.

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