Journal of Diagnostics Concepts & Practice ›› 2018, Vol. 17 ›› Issue (03): 272-277.doi: 10.16150/j.1671-2870.2018.03.008

• Original articles • Previous Articles     Next Articles

Correlation between brown adipogenic capacity of preadipocytes and ageing and the related mechanism in mice

PAN Xiaoxi, CAO Jiumei, WU Fang   

  1. Department of Geratology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Received:2018-01-06 Online:2018-06-25 Published:2018-06-25

Abstract: Objective: To investigate correlation between brown adipogeneic capacity of preadipocytes and ageing and the related mechanism in mice. Methods: Preadipocytes derived from various age mice were cultured in vitro, and oil red O staining was used to identify the brown adipogenic capacity. The expression of miR-146b-3p in young and old preadipocytes was detected by real-time quantitative PCR. Then miR-146b-3p inhibitors were transfected into young preadipocytes, while miR-146b-3p mimics were transfected into the old, and the relative mRNA levels of PLIN, FABP4, UCP-1, APN were measured by real-time quantitative PCR. Results: Preadipocytes of aged mice showed a weaker capacity of brown adipogenic capacity than that of the young mice, and the expression of miR-146b-3p in the preadipocytes of the aged group was also decreased. Overexpression of miR-146b-3p can improve the brown adipogenic capacity of preadipocytes in aged mice. However, the brown adipogenic capacity of the young declined with the decrease of miR-146b-3p expression. Conclusions: The brown adipogenic capacity of preadipocytes decreases with age, and miR-146b-3p might be one of the targets.

Key words: miR-146b-3p, Ageing, Preadipocytes, Brown adipocytes

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