Loading...

Table of Content

    25 June 2018, Volume 17 Issue 03 Previous Issue    Next Issue
    For Selected: Toggle Thumbnails
    Original articles
    Study on the involving of Fra2 via regulating IL23 receptor in the pathogenesis of psoriasis
    LIN Zhen, GUO Ruru, LÜ Liangjing, CHEN Xiaoxiang
    2018, 17 (03):  254-259.  DOI: 10.16150/j.1671-2870.2018.03.005
    Abstract ( 438 )   PDF (724KB) ( 122 )  
    Objective: To study the expression of Fra2, a member of Fos family of transcription factor AP1 family, in peripheral blood mononuclear cells (PBMC) of psoriatic patients, and to analyze the potential genes regulated by Fra2 via bioinformatics and confirmed by dual-luciferase reporter assay for investigating the role of Fra2 in the pathogenesis of psoriasis. Methods: The expression of Fra2 in 21 patients with psoriasis and 30 healthy controls were detected by quantitative real-time-PCR(qrt-RCR). The target genes of Fra2 were predicted by gene transcription regulation Database (GTRD), and were intersected with psoriasis-related gene in GeneCards. The function classification of Gene Ontology (GO)and KEGG enrichment analysis were adopted by DAVID (The Database for Annotation, Visualization and Integrated Discovery); the potential genes regulated by Fra2 were verified by qrt -PCR; the binding sites of Fra2 were predicted by JASPAR package in R Programming Language;the relationship between Fra2 and IL23 receptor(IL23R) was verified by Dual-luciferase report assay. Results: The expression of Fra2 mRNA in PBMC of 21 patients with psoriasis (0.711±0.072) was significantly lower than that in healthy controls (1.070±0.086) (P=0.004 2). Bioinformatics analysis showed that inflammatory response and cytokine-cytokine receptor interaction was the most significant items(P=5.2×10-71; P=7.0×10-45). qrt-PCR found that the mRNA expression level of IL23R was significantly higher than healthy controls (P=0.000 1), and was correlated with Fra2 expression level(r=-0.509 5, P=0.018 3); the regulating effect of Fra2 on IL23R was verified by Dual-luciferase report assay. Conclusions: Fra2 is an important transcription factor involved in the development and progress of psoriasis by regulating the expression of IL23R.
    References | Related Articles | Metrics
    Molecular mechanism of Ax subtype caused by p.M142I mutation in alpha 1-3-N-acetylgalactosaminyltransferase
    WANG Dengfeng, CUI Wenyan, ZOU Wei, LI Fang, WANG Xuefeng, CAI Xiaohong
    2018, 17 (03):  260-265.  DOI: 10.16150/j.1671-2870.2018.03.006
    Abstract ( 338 )   PDF (887KB) ( 96 )  
    Objective: To explore the potential molecular mechanism of Ax subtypes in Chinese population based on an Ax subtype family. Methods: The ABO blood group serological tests and detection of the total plasma activity of alpha α-1,3-N-acetylgalactosaminyltransferase were conducted. ABO gene was sequenced directly and sequenced after cloning to construct 3D molecular model, and then the effect of identified mutation on protein stability changes (ΔΔG) of alpha α-1,3-N-acetylgalactosaminyltransferase was predicted. Results: The proband was diagnosed as AxB subgroup byserological analysis, and AW.38/B.01 was identified by DNA analysis. His two daughters were identified as A and AB blood group, and their ABO genotypes were A1.02/B.01, A1.02/AW.38, respectively.There was a c.426G>C heterozygous mutation in ABO exon 7 of the proband and his A type daughter, leading to p.M142I change in the amino acid of α-1,3-N-acetylgalactosaminyltransferase. Molecular modeling and analysis suggested that the mutation may lead to changes in the number of hydrogen bonds formed. The increase of ΔΔG value of thermodynamic stability changes indicated that the protein stability was reduced. Conclusions: The mutation of p.M142I in α-1,3-N-acetylgalactosaminyltransferase may lead to Ax phenotype by reducing the stability of the enzyme. In vitro study is needed for further investigation.
    References | Related Articles | Metrics
    Distribution of pathogenic bacteria in neonatal bloodstream infection and analysis of drug resistance during 2014 to 2017
    SHEN Xiaohong, CHEN Huifen, ZHANG Jun, YE Jianbo, ZHANG Xianhua
    2018, 17 (03):  266-271.  DOI: 10.16150/j.1671-2870.2018.03.007
    Abstract ( 517 )   PDF (606KB) ( 75 )  
    Objective: To study the distribution of pathogenic bacteria and drug resistance in neonatal bloodstream infection for providing a reference for the rational use of antimicrobialagents. Methods: The distribution of pathogenic bacterial strains isolated from blood culture specimens of neonates and its drug resistance at Shanghai First Maternity and Infant Hospital from 2014 to 2017 were analyzed retrospectively. Results: A total of 500 1 blood specimens collected from in-patient neonates from 2014-2017 were cultured.Among them, 153 cases of neonatal bloodstream infection were detected and 155 strains of bacteria were isolated. Of these strains there were 88 strains of Gram-positive bacteria,mainly composed of 38 strains of coagulase-negative Staphylococci (CNS), 20 strains of S.agalactiae, 18 strains of Staphylococcus aureus; 57 strains were Gram-negative bacteria strains, mainly composed of 18 strains of Escherichia coli,17 strains of Klebsiella pneumoniae; and 10 strains were fungi. Of these cases 54 were neonatal early-onset blood stream infection, the main pathogens were S.agalactiae (27.27%) and E. coli(21.82%); 99 cases were neonatal late-onset blood stream infection, the main pathogens were coagulase-negative Staphylococcus (25.00%), Staphylococcus aureus(17%) and K. pneumoniae (15%). Thirty eight of the 56 Staphylococci strains were identified as methicillin-resistant Staphylococcus. S.agalactiae had higher resistance to clindamycin, erythromycin and tetracycline, the resistance rates were 95.00%, 85.00 and 95.00%, respectively. E. coli and K. pneumoniae had higher resistance to ampicillin, piperacillin, and cephalosporin, the resistance rates were 88.57%, 62.86%, 62.86%, respectively, the detection rate of ESBLs-producing strains was 60%. Conclusions: S.agalactiae and E. coli are the main pathogens of neonatal early-onset bloodstream infection, while coagulase negative stapylococci, Staphylococcus aureus and K. pneumoniae are the main pathogens of neonatal late-onset bloodstream infection. Higher drug resistance is found in pathogens of neonatal late-onset bloodstream infection.
    References | Related Articles | Metrics
    Correlation between brown adipogenic capacity of preadipocytes and ageing and the related mechanism in mice
    PAN Xiaoxi, CAO Jiumei, WU Fang
    2018, 17 (03):  272-277.  DOI: 10.16150/j.1671-2870.2018.03.008
    Abstract ( 365 )   PDF (784KB) ( 89 )  
    Objective: To investigate correlation between brown adipogeneic capacity of preadipocytes and ageing and the related mechanism in mice. Methods: Preadipocytes derived from various age mice were cultured in vitro, and oil red O staining was used to identify the brown adipogenic capacity. The expression of miR-146b-3p in young and old preadipocytes was detected by real-time quantitative PCR. Then miR-146b-3p inhibitors were transfected into young preadipocytes, while miR-146b-3p mimics were transfected into the old, and the relative mRNA levels of PLIN, FABP4, UCP-1, APN were measured by real-time quantitative PCR. Results: Preadipocytes of aged mice showed a weaker capacity of brown adipogenic capacity than that of the young mice, and the expression of miR-146b-3p in the preadipocytes of the aged group was also decreased. Overexpression of miR-146b-3p can improve the brown adipogenic capacity of preadipocytes in aged mice. However, the brown adipogenic capacity of the young declined with the decrease of miR-146b-3p expression. Conclusions: The brown adipogenic capacity of preadipocytes decreases with age, and miR-146b-3p might be one of the targets.
    References | Related Articles | Metrics
    Intraductal papillary mucinous neoplasm with an associated invasive carcinoma of the pancreas: analysis of the clinicopathologic features and prognosis
    WANG Ting, XIE Wen, LIN Xiaozhu, YUAN Fei, WANG Chaofu, GUO Yan
    2018, 17 (03):  278-284.  DOI: 10.16150/j.1671-2870.2018.03.009
    Abstract ( 634 )   PDF (1246KB) ( 239 )  
    Objective: To evaluate the clinicopathologic features and prognosis of intraductal papillary mucinous neoplasm with an associated invasive carcinoma of the pancreas. Methods: The clinical data, pathological features and prognosis of 52 patients were studied retrospectively. Results: Of all the 52 cases, 36 lesions were located in the head of pancreas, 15 were in the body/tail, and 1 was in the entire length of the pancreatic duct. The median age of these patients was 65 years. Of the 52 IPMNs, 23 cases were the gastric type, 12 cases were intestinal, 9 cases were pancreatobiliary, 7 cases were mixed type (gastric+pancreatobiliary type) and 1 case was oncocytic. The types of invasive carcinoma included 37 cases of ductal adenocarcinomas, 13 cases of colloid carcinomas, 1 cases of undifferentiated carcinoma and 1 cases of adenosquamous carcinoma. Elevated serum CA19-9 level was more frequent in patients with ductal adenocarcinoma. Nine of 52 patients were lost to follow-up. The median postoperative follow-up period was 16 months (range 5-57 months). Conclusions: The intraductal papillary mucinous neoplasm with an associated invasive carcinoma of the pancreas are mainly ductal adenocarcinoma. Elevated serum CA19-9 level is associated with the types of the invasive carcinoma. The positive lymph nodes and tumor extending beyond pancreas are risk factors for the poor prognosis.
    References | Related Articles | Metrics
    Analysis on levels of vitamins in middle-aged and elderly patients with diabetes mellitus in Shanghai
    JI Pengcheng, ZHANG Shu, SUN Jing, JIANG Shihu
    2018, 17 (03):  285-289.  DOI: 10.16150/j.1671-2870.2018.03.010
    Abstract ( 362 )   PDF (520KB) ( 88 )  
    Objective: To investigate the changes in vitamin levels in middle aged and elderly patients with diabetes mellitus in Shanghai. Methods: A total of 1 100 middle aged and elderly participants undergone health examination were enrolled during January 2016 to November 2016. Of these participants, 264 diabetic patients were categorized into the diabetic group and the other 836 non-diabetes were served as the control group. All the subjects were tested for vitamins (vitamin A, B1, B2, B6, B12, C, E and folic acid) and glycosylated hemoglobin (HbA1c), and differences in vitamin levels between diabetic group and control group were compared. According to the level of glycosylated hemoglobin (HbA1c), patients with type 2 diabetes mellitus were further divided into 2 groups: well-controlled group(HbA1c<7.0%) and poor-controlled group (HbA1c≥7.0%). The correlation of HbA1c with vitamins in the body was assessed. Results: Compared with controls, diabetes mellitus patients had a lower serum vitamin B12 level [(408.82±163.53) pg/mL vs (437.49±206.82) pg/mL P<0.05]. Patients with HbA1c≥7.0% had lower level of vitamin B12 than patients with HbA1c<7.0%[(372.89±129.68) pg/mL vs(422.30±172.92) pg/mL, P<0.05]. Linear correlation analysis showed that level of glycated hemoglobin had no correlation with serum levels of other vitamins (all P>0.05). Conclusions: Compared with non-diabetes, patients with diabetes have lower serum vitamin B12 level, and poor controlled diabetic patients have lower serum vitamin B12 level than well-controlled diabetic patients.
    References | Related Articles | Metrics
    Blood tests before transfusion and therapeutic evaluation of autoimmune hemolytic anemia patients: Analysis of 2 cases
    WANG Chengyun, ZHANG Fan, GU Ping, PAN Qiuhui, WANG Jing
    2018, 17 (03):  290-293.  DOI: 10.16150/j.1671-2870.2018.03.011
    Abstract ( 510 )   PDF (455KB) ( 118 )  
    Objective: To explore the importance of pre-transfusion detection and blood selection for therapeutic efficacy in autoimmune hemolytic anemia. Methods: Blood group identification,irregular antibody screening and cross-ma-tching were performed with DG Gel confirmcards, Neutral cards, Coombs cards and WADiana/8XT Compact Analyzer (Diagnostic Gri-fols, S.A). Thetransfusion efficacy was assessed. Results: Both samples from the 2 patients showed agglutination of O cell and positive antibody screening test. The mimicking anti-C was identified in one sample based on the response pattern of panel cells, and autoantibody of another sample was not clearly identified. The health condition of both patients was improved after transfusing with red blood cells and the transfusion therapy was effective. Conclusions: In patients with autoimmune hemolytic anemia, the irregular antibodies were rather complex, therefore the blood cells without associa-ted antigen should be selected for transfusion according to the character of antibody. If the autoantibody showed mimicking Rh blood group characteristics, blood cells without the associated antigen were selected for transfusion. If the autoantibody was not clearly identified, blood cells with the same ABO blood group should be selected for the compatible test and that with the weakest reaction was chosen from multiple donors. If direct antiglobulin test was positive, especially the anti-C3d positive, donor's blood should be washed before transfusion to avoid hemolysis caused by the residual complement.
    References | Related Articles | Metrics
    Expression of PD-1 on peripheral blood Treg cells in patients with primary immune thrombocytopenia
    LI Tan, LI Bin, LIU Yuanyuan, BAO Yangyi
    2018, 17 (03):  294-298.  DOI: 10.16150/j.1671-2870.2018.03.012
    Abstract ( 324 )   PDF (517KB) ( 78 )  
    Objective: To investigate the proportion of regulatory T cells(Treg cells) in peripheral blood and the expression of programmed death receptor-1 (PD-1) on Treg cells in adult primary immune thrombocytopenia(ITP) patients, and to analyze their clinical significance. Methods: Altogether 30 patients with ITP were enrolled and divided into effective group(21 patients)and ineffective group(9 patients)according to whether platelets returned to normal level after treatment. Flow cytometry(FCM) was applied to determine the counts of CD3+ T lymphocytes, and proportions of Treg cells and PD-1+ Tregcells in ITP patients pre- and post-treatment. Results: The count of CD3+ T lymphocytes was not differed significantly before and after treatment in both the effective treatment group [6.39±5.74)×109/L vs (5.49±4.86)×109/L] and ineffective group[(8.34±2.95)×109/L vs (7.87±1.47)×109/L]. The proportion of Treg cells increased from 16.96%±4.61% to 32.74%±9.38% in effective treatment group(P<0.05), and the proportion of PD-1+ Treg cells increased from 14.88%±6.75% to 29.62%±7.97%(P<0.05). In contrast, Treg cells increased a little and maintained at a low level in ineffective group (from 17.41%±6.77% to 19.18%±7.64%, P>0.05) and PD-1+ Treg had no significant increase (from 15.30%±6.57% to 19.58%±6.91%, P>0.05). Moreover, the proportions of peripheral blood Treg cells and PD-1+ Treg cells in the effective group were significantly higher than those in the ineffective group after treatment (P<0.05). Conclusions: Treg cells and PD-1+ Treg cells may play an important role in the development and progress of ITP. The detection of proportion of these two cells in peripheral blood might be helpful to predict the prognosis of ITP.
    References | Related Articles | Metrics
    Correlation of serum interleukin 33 and soluble suppression of tumorigenicity 2 with injury severity and prognosis in trauma patients
    YAO Yulan, GUO Enwei, REN Dali, ZHANG Bingyu, YANG Feng, CHEN Qing, LIU Xinghui, LUO Qiancheng, FENG Gang
    2018, 17 (03):  299-303.  DOI: 10.16150/j.1671-2870.2018.03.013
    Abstract ( 340 )   PDF (479KB) ( 63 )  
    Objective: To investigate the correlation of serum interleukin 33(IL-33)and its receptor soluble suppression of tumorigenicity 2(sST2) with injury severity score(ISS) and prognosis in trauma patients. Methods: According to ISS, 67 trauma patients were divided into 2 groups, mild trauma group (ISS<20 points)and severe trauma group(ISS≥20 points).Serum levels of IL-33, sST2 were measured at 4 h, 24 h, 72 h, 7 d after trauma, and mortality in 28 days were compared between mild trauma group and severe trauma group .Serum levels of IL- 33 and sST2 were compared between the survival and the dead patients. The receiver operating characteristic(ROC) curve was used to determine the cutoff value of indices for predicting the death of patient. Results: The levels of serum IL-33 and sST2 were significantly higher in severe trauma patients than those with mild trauma at 4 h and 24 h [IL-33, (38.75±28.43) pg/mL vs (19.62±9.98) pg/mL, (42.31±37.37) pg/mL vs (23.47±13.42) pg/mL, P<0.05; sST2,(6.50±3.74) ng/mL vs (4.89±2.40) ng/mL, (8.35±2.69) ng/mL vs (6.78±3.22) ng/mL, P<0.05]. The levels of serum IL-33 and sST2 were significantly higher in dead patients than in survival patients during 24 h-7d after trauma (P<0.01). The area under ROC curve of serum IL-33 and sST2 levels at 24 h after trauma were 0.799 (P=0.003) and 0.751( P=0.012), respectively. The optimal cutoff value of serum IL-33 and sST2 levels at 24 h after trauma were 15.27 pg/mL and 10.99 ng/mL respectively, and the sensitivity and specificity for predicting death were 100% and 70%,and 64% and 83%, respectively. Conclusions: Elevated levels of IL-33 and sST2 are associated with poor prognosis in patients with trauma, and serum IL-33 and sST2 at 24 h after trauma could be used as biomarkers for predicting prognosis in patients with trauma.
    References | Related Articles | Metrics
    Value of IP-10 combined with PCT and hs-CRP for the diagnosis of neonatal infection
    LU Shijuan, SHANG Qingyi, JIANG Zhihong, CHEN Tongpai, ZHANG Xiaoqing
    2018, 17 (03):  304-307.  DOI: 10.16150/j.1671-2870.2018.03.014
    Abstract ( 409 )   PDF (419KB) ( 93 )  
    Objective: To investigate the value of interferon-induced protein 10 (IP-10) combined with procalcitonin (PCT) and hypersensitive C-reactive protein (hs-CRP) for the diagnosis of neonatal infection. Methods: IP-10 was detected by enzyme-linked immunosorbent assay, PCT was detected by double antibody sandwich immune chemiluminescence method, and hs-CRP was detected by immunoturbidimetric assay. The serum levels of IP-10, PCT and hs-CRP of newborn infants were determined at admission in 69 cases with infection, including 43 cases of bacterial infection and 26 cases of viral infection, and in 30 cases of control group. The cases in infection group were also detected after 2 days of treatment and after being cured. Results: The levels of IP-10, PCT and hs-CRP in bacterial infection group before treatment were (89.39±23.09) pg/mL, (2.08±0.30) μg/L and (10.49±6.97) mg/L, respectively, significantly higher than that in the control group (42.03±14.80) pg/mL, (0.27±0.30) μg/L and (5.39±4.20) mg/L, respectively (P<0.05). The level of IP-10 in viral infection group before treatment (62.91±6.79 pg/mL) was also higher than that in the control group but lower than that in the bacterial infection group (P<0.05). After 2 days of treatment and after being cured, levels of all the three markers were significantly decreased than that before treatment in both the two infection groups (P<0.05). The ROC curve analysis for the single marker and combined 3 markers indicated that the sensitivity and specificity of IP-10 (92.3% and 94.0%) and PCT (93.1% and 9.2%) were high, while that of hs-CRP were lower (65.5% and 2.6%). The sensitivity and specificity were elevated when the 3 markers were combined (95.6% and 6.8%). Conclusions: IP-10 and PCT has a high value for predicting early bacterial infection, and IP-10 has a value for predicting viral infection. The value of hs-CRP for identifying neonatal infection is lower than that of IP-10 and PCT. The combination of the three markers further elevates the diagnostic value for detecting newborn infection.
    References | Related Articles | Metrics
    The status of Streptococcus agalactiae infection and drug resistance in Shanghai from 2015 to 2017
    CAO Xueping, ZHANG Xianhua, CHEN Huifen, SHEN Pinghua
    2018, 17 (03):  308-310.  DOI: 10.16150/j.1671-2870.2018.03.015
    Abstract ( 447 )   PDF (348KB) ( 192 )  
    Objective: To investigate the status of Streptococcus agalactiaeS.agalactiae)infection and drug resistance in Shanghai from 2015 to 2017. Methods: A retrospective analysis was performed on the positive rate of S.agalactiae in departments of obstetrics, gynecology and andrology at Shanghai First Maternal and Infant Hospital from 2015 to 2017, and antimicrobial susceptibility test was performed on 374 strains of S. agalactiae isolated in 2017. D-test was performed for strains resistant to erythromycin and susceptible to clindamycin. Results: In 2015, the positive rates of S.agalactiae at departments of obstetrics, gynecology and andrology were 2.69%(88/3 267), 3.10%(101/3 255), 5.93%(28/472), respectively; in 2016 were 3.94%(378/9 578), 3.42%(92/2 685), 7.49%(14/187) respectively, and in 2017 were 3.90%(251/ 6 429), 4.10%(110/2 682), 7.22%(13/180), respectively. The positive rates of S.agalactiae in department of obstetrics from 2015 to 2017 was 3.72% (717/19 274). In 2017, the resistance rates of S.agalactiae to erythromycin, clindamycin and levofloxacin were 63.37% (237/374), 53.48% (200/374) and 25.13% (94/374), respectively. None of the strains were found resistant to penicillin, ceftriaxone, linezolid and vancomycin. Conclusions: The infection of S.agalactiae has a trend of increasing from 2015 to 2017, which should be paid attention seriously. It is important to perform screening of S.agalactiae in pregnant women and non-pregnant adult patients. Penicillins are still the first choice for the treatment of S.agalactiae infection.
    References | Related Articles | Metrics
    Animal and in vitro study on mechanism of berberine alleviating high cholesterol induced liver injury
    SHAO Wentao, SUN Haidong, WANG Qihan, LIU Qian, JIANG Zhaoyan, GU Aihua
    2018, 17 (03):  311-317.  DOI: 10.16150/j.1671-2870.2018.03.016
    Abstract ( 367 )   PDF (1263KB) ( 92 )  
    Objective: To investigate the protective effect of berberine (BBR) on liver damage under high-cholesterol diet challenge. Methods: Eight-week old male C57BL/6J mice were randomly divided into normal diet group, high cholesterol diet (1.25% cholesterol+0.5% cholic acid) group and high cholesterol diet+BBR (100 mg/kg per day by gavage) group, with 10 mice in each group. Liver and serum plasma samples were collected after 8-week feeding. Hepatic mRNA expressions of genes involved in endoplasmic reticulum stress (ER stress), oxidative stress and inflammatory cytokines were determined by real-time quantitative PCR, and content of malondialdehyde (MDA) in liver tissues was measured. HepG2 cell line was used to investigate the effect of BBR on ER stress under high cholesterol challenge. Results: Compared with normal diet group, hepatic mRNA expressions of genes in ER stress, oxidative stress and inflammatory cytokines increased significantly in high cholesterol diet group (P<0.05), and were reduced closely to levels of normal diet group by BBR treatment (P<0.05). BBR also down-regulated the increased hepatic MDA content induced by high cholesterol diet (P<0.05). Furthermore, BBR decreased the expression of GRP78, a key protein marker concerning ER stress (P<0.05), as well as the fluorescence intensity of DHE probe after high cholesterol treatment in HepG2 cells. Conclusions: BBR could effectively ameliorate hepatic ER stress, oxidative stress and inflammatory response under high cholesterol challenge, which might protect liver from damage when overloaded with cholesterol.
    References | Related Articles | Metrics
    Study on angiogenic properties of endothelial progenitor cells in low birth weight preterm infants
    DING Xiaowei, ZHANG Ruyuan, LI Ranran, TANG Haiting
    2018, 17 (03):  318-321.  DOI: 10.16150/j.1671-2870.2018.03.017
    Abstract ( 312 )   PDF (512KB) ( 161 )  
    Objective: To investigate the angiogenic properties of cord blood endothelial progenitor cells (EPCs) in low birth weight(LBW) preterm infants. Methods: Venous umbilical cord blood was collected from 19 cases of full-term neonates with normal weight and 15 LBW neonates. The relative number of cord blood EPCs (CD34+/CD133+/VEGFR2+) from LBW preterm and full-term neonates was counted by ?ow cytometry. The angiogenic properties of cord blood EPCs were evaluated for the time for clone formation, clone forming ability and capacity to proliferate and formtubes in vitro. Results: There was no difference in the relative number of cord blood EPCs between LBW preterm and full-term neonates. Compared with EPCs of full-term neonates, the time for clone formation of EPCs was delayed in LBW, while clone forming ability and capacity to proliferate and form tubes were decreased. The expressions of VEGFR2 and VE-cadherin of EPCs in LBW were significantly reduced. Conclusions: Angiogenic properties of EPCs are impaired in LBW preterm infants, and this may be due to the decreased expression of VE-cadherin and VEGFR2.
    References | Related Articles | Metrics
    Genotypes of HCV among 276 patients with hepatitis C virus infection in People's Hospital of Dehong Prefecture, Yunnan Province
    LIU Houchang, PAN Yunhua, ZHANG Kai, WANG Shuihua
    2018, 17 (03):  322-327.  DOI: 10.16150/j.1671-2870.2018.03.018
    Abstract ( 408 )   PDF (1090KB) ( 89 )  
    Objective: To explore the distribution of hepatitis C virus (HCV) genotypes in Dehong, Yunnan. Methods A total of 276 serum samples from out-and in-patients with positive HCV RNA in People's Hospital of Dehong Prefecture from January 2014 to March 2017 were collected, and PCR-reverse dot hybridization was used to determine the HCV genotypes, including genotypes of 1a, 1b, 2a, 3a, 3b and type 6. Those samples with uncertain results were further detected by NS5B (nonstructural protein 5B) sequencing typing. Results Of the 276 samples, 120 samples (43.48%) were identified as HCV subtype 6, 80 samples (28.98%), as 3b, 33 samples (11.96%) as 1b, 30 samples (10.87%) as 3a, 9 samples (3.26%) as 1a, and 4 samples (1.45%) as 2a. Among all the patients, 151 patients were local cases, 61 samples (40.40%) were identified as HCV genotype subtype 6, 53 samples (35.10%), as 3b 15 samples (9.93%) as 1b, 14 samples (9.27%) as 3a, 4 samples (2.65%) as 1a, and 4 samples (2.65%) as 2a, and distribution of genotyping was different from those in other places in China (P<0.05), with a higher prevalence of subtype 6 and 3 and lower prevalence of subtype of 1a and 2a. Conclusions A total of 9 subtypes of HCV gene are detected in Dehong, Yunnan province, and the main types are 3b and 6 (including 6n, 6u, 6a,6m, 6x), followed by 1b and 3a. When compared with other provinces of China, distribution of HCV genotype in Dehong has its unique characteristic.
    References | Related Articles | Metrics
    Application of multiplex ligation-dependent probe amplification (MLPA) in analyzing chromosome aneuploid abnormalities of embryo arrest
    QIN Yunrong, NING Sisi, LU Yinghong
    2018, 17 (03):  328-332.  DOI: 10.16150/j.1671-2870.2018.03.019
    Abstract ( 335 )   PDF (738KB) ( 118 )  
    Objective: To explore the application of multiplex ligation-dependent probe amplification (MLPA) for the detection of chromosome aneuploid abnormalities in chorionic villus samples from embryo arrest, and to analyze the correlation of fetal gender and maternal age with chromosome aneuploid abnormalities. Methods A total of 324 chorionic villus samples from embryo arrest were collected and chromosome aneuploid abnormalities were detected with MLPA. Correlation of chromosome aneuploid abnormalities with fetal gender as well as maternal age was analyzed. Results Among the 324 samples, 68 cases (20.99%) were detected as having chromosome aneuploid abnormalities, including 33 cases of autosomal trisomy (12 cases of 13-trisomy, 6 cases of 18-trisomy, 15 cases of 21-trisomy), 3 cases of autosomal deletion (1 cases of 13-monosomy, 2 cases of 21-monosomy, 15 cases of 21-trisomy) and 32 cases of sex chromosome number abnormality(15 cases of 45-XO, 13 cases of 47-XXY, 1 cases of 47-XXX, 3 cases of 47-XYY). There was no significant difference in fetal gender distribution between the abnormal autosomal number group and the normal autosomal number group (P>0.05). The differences in maternal age among the normal autosomal number group and abnormal autosomal number groups and abnormal sex chromosome number group were statistically significant (P<0.05). Conclusions MLPA technique can be used as an accessory approach for detection of chromosome aneuploid abnormalities. The chromosomal number abnormalities is not related to fetal gender, but is related with the maternal age, and the maternal age of those with abnormal autosomal number is higher than those with normal autosomal number group and abnormal sex chromosome number group.
    References | Related Articles | Metrics
    Value of CT in imaging diagnosis of hepatic hydatidosis and analysis of misdiagnosis
    LI Jing, FENG Jun, WANG Ruonan, SHI Haifeng
    2018, 17 (03):  333-336.  DOI: 10.16150/j.1671-2870.2018.03.020
    Abstract ( 473 )   PDF (538KB) ( 257 )  
    Objective: To explore the value of CT for diagnosing hepatic hydatidosis. Methods: A total of 18 cases with pathologically confirmed hepatic hydatidosis were enrolled, and CT features were retrospectively analyzed with the pathological findings served as gold standard. Results: Of the 18 cases, 14 cases were diagnosed as hepatic hydatidosis by CT preoperatively, with an accuracy rate of 77.8%, while 2 cases were misdiagnosed as hepatic cyst, 1 case as liver carcinoma, and 1 case as hepatic hemangioma. For the 17 cases with cystic echinococcosis, 9 cases were of simple cystic type, lesions on CT imaging were presented as single or multiple low density foci, cyst wall arc or eggshell like calcification; 6 cases had echinococcus hydatid sac in cysts with changes of wheeled or honeycomb, and might presented as mixtures of lesions. CT imaging revealed that cystic wall was thicker and cystic density increased when there was infection. The one case of alveolar echinococcosis was misdiagnosed as liver carcinoma, with a wide range of lesions in liver and spread of lesion to bilateral lung on CT imaging. Two cases with single cyst were misdiagnosed as hepatic cyst because CT showed no cyst wall and without obvious contrast enhancement. Conclusions: CT scan can clearly reveal the location, size, number and type of hepatic echinococcosis, which could provide valuable information for diagnosis. Differential diagnosis should be made between hepatic echinococcosis and hepatic cyst when lesions present as simple cysts on CT imaging. CT findings of alveolar echinococcosis are complicated, and should be differentiated from those of liver cancer.
    References | Related Articles | Metrics