Journal of Diagnostics Concepts & Practice >
Correlation between imbalance of T lymphocyte subsets and symptom fluctuation in patients with myasthenia gravis
Received date: 2018-11-05
Online published: 2019-04-25
Objective: To investigate the relationship between changes of T lymphocytes and exacerbation and remission in patients with generalized myasthenia gravis (GMG). Methods: Flow cytometry was used to detect the change of T lymphocyte subsets in peripheral blood of 51 patients with GMG during different periods of exacerbation and remission, and analyzed the relationship between the changes of T lymphocytes and symptom fluctuations. Results: The percentage of CD4+CD25+CD127low/-regulatory T lymphocytes and CD4+CD45RA+naive T lymphocytes was significantly lower in exacerbation period than that in control group (P<0.05), whereas the percentage of CD4+CD45RO+ memory T lymphocytes in exacerbation period was significantly higher than that in remission period and control group(P<0.05). The AUC of CD4+CD25+CD127low/-regulatory T lymphocytes and CD4+CD45RA+naive T lymphocytes were 0.713 and 0.900, respectively. The AUC of CD4+CD45RO+ memory T lymphocytes in exacerbation period was 0.675 and the optimum critical value was 32.06%. Conclusions: Regulatory T lymphocytes and CD4+CD45RA+naive T lymphocytes have diagnostic value for GMG. CD4+CD45RO+ memory T lymphocytes are correlated with symptom aggravation and remission of GMG, and may be used as a marker for measuring the symptom fluctuation of myasthenia gravis.
Key words： Myasthenia gravis; T lymphocyte subsets; Flow cytometry
LAI Xiaoyin, SUN Jialan, HU Rongguo, YANG Xuelian, WU Guolu, LI Longxuan, BU Bitao . Correlation between imbalance of T lymphocyte subsets and symptom fluctuation in patients with myasthenia gravis[J]. Journal of Diagnostics Concepts & Practice, 2019 , 18(2) : 199 -203 . DOI: 10.16150/j.1671-2870.2019.02.015
| [1] | Jeong A, Min JH, Kang YK, et al. Factors associated with quality of life of people with Myasthenia Gravis[J]. PLoS One, 2018, 13(11):e0206754. |
| [2] | Berrih-Aknin S, Le Panse R. Myasthenia gravis: a comprehensive review of immune dysregulation and etiological mechanisms[J]. J Autoimmun, 2014, 52:90-100. |
| [3] | Gradolatto A, Nazzal D, Truffault F, et al. Both Treg cells and Tconv cells are defective in the Myasthenia gravis thymus: roles of IL-17 and TNF-α[J]. J Autoimmun, 2014, 52:53-63. |
| [4] | Walter GJ, Fleskens V, Frederiksen KS, et al. Phenoty-pic, Functional, and Gene Expression Profiling of Peripheral CD45RA+ and CD45RO+ CD4+CD25+CD127(low) Treg Cells in Patients With Chronic Rheumatoid Arthritis[J]. Arthritis Rheumatol, 2016, 68(1):103-116. |
| [5] | Liu C, Wang Q, Qiu Z, et al. Analysis of mortality and related factors in 2195 adult myasthenia gravis patients in a 10-year follow-up study[J]. Neurol India, 2017, 65(3):518-524. |
| [6] | Liu J, Lu CX, Zhang F, et al. Expression of ILT3 predicts poor prognosis and is inversely associated with infiltration of CD45RO+ T cells in patients with colorectal cancer[J]. Pathol Res Pract, 2018, 214(10):1621-1625. |
| [7] | Attia M, Maurer M, Robinet M, et al. Muscle satellite cells are functionally impaired in myasthenia gravis: consequences on muscle regeneration[J]. Acta Neuropathol, 2017, 134(6):869-888. |
| [8] | 荣举, 翁文娟, 林晓瑜, 等. 系统性红斑狼疮患者外周血细胞CD45分子亚型分析[J]. 汕头大学医学院学报, 2016, 29(4):215-217. |
| [9] | Alahgholi-Hajibehzad M, Kasapoglu P, Jafari R, et al. The role of T regulatory cells in immunopathogenesis of myasthenia gravis: implications for therapeutics[J]. Expert Rev Clin Immunol, 2015, 11(7):859-870. |
| [10] | Shin DS, Jordan A, Basu S, et al. Regulatory T cells suppress CD4+ T cells through NFAT-dependent transcriptional mechanisms[J]. EMBO Rep, 2014, 15(9):991-999. |
| [11] | Thiruppathi M, Rowin J, Ganesh B, et al. Impaired regulatory function in circulating CD4(+)CD25(high)CD127(low/-) T cells in patients with myasthenia gravis[J]. Clin Immunol, 2012, 145(3):209-223. |
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